Gene description for Lars
Gene name leucyl-tRNA synthetase
Gene symbol Lars
Other names/aliases 2310045K21Rik
3110009L02Rik
AW536573
mKIAA1352
Species Mus musculus
 Database cross references - Lars
ExoCarta ExoCarta_107045
Vesiclepedia VP_107045
Entrez Gene 107045
UniProt Q8BMJ2  
 Lars identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lars
Molecular Function
    aminoacyl-tRNA editing activity GO:0002161 ISO
    aminoacyl-tRNA editing activity GO:0002161 ISS
    glutamine-tRNA ligase activity GO:0004819 IEA
    glutamine-tRNA ligase activity GO:0004819 ISO
    leucine-tRNA ligase activity GO:0004823 IBA
    leucine-tRNA ligase activity GO:0004823 IMP
    leucine-tRNA ligase activity GO:0004823 ISO
    leucine-tRNA ligase activity GO:0004823 ISS
    GTPase activator activity GO:0005096 IEA
    GTPase activator activity GO:0005096 ISO
    ATP binding GO:0005524 IEA
Biological Process
    glutaminyl-tRNA aminoacylation GO:0006425 IEA
    glutaminyl-tRNA aminoacylation GO:0006425 ISO
    leucyl-tRNA aminoacylation GO:0006429 IBA
    leucyl-tRNA aminoacylation GO:0006429 IDA
    leucyl-tRNA aminoacylation GO:0006429 ISO
    regulation of cell size GO:0008361 IEA
    regulation of cell size GO:0008361 ISO
    positive regulation of TOR signaling GO:0032008 ISO
    cellular response to amino acid starvation GO:0034198 ISO
    positive regulation of GTPase activity GO:0043547 ISO
    cellular response to amino acid stimulus GO:0071230 ISO
    cellular response to L-leucine GO:0071233 IEA
    cellular response to L-leucine GO:0071233 ISO
    aminoacyl-tRNA metabolism involved in translational fidelity GO:0106074 IEA
    positive regulation of TORC1 signaling GO:1904263 IEA
    positive regulation of TORC1 signaling GO:1904263 ISO
    cellular response to leucine starvation GO:1990253 IEA
    cellular response to leucine starvation GO:1990253 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    endomembrane system GO:0012505 ISO
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IDA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 ISO
 Experiment description of studies that identified Lars in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lars
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dhx29  
Co-fractionation Mus musculus
2 Rpl13a 22121
Co-fractionation Mus musculus
3 Ubr4 69116
Co-fractionation Mus musculus
4 Hars2  
Co-fractionation Mus musculus
5 Rps2 16898
Co-fractionation Mus musculus
6 Thnsl2  
Co-fractionation Mus musculus
7 Rpl7a 27176
Co-fractionation Mus musculus
8 Atxn1  
Affinity Capture-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Tmem165  
Co-fractionation Mus musculus
11 Arg1  
Co-fractionation Mus musculus
12 Ywhae 22627
Affinity Capture-MS Mus musculus
13 Rpl22 19934
Co-fractionation Mus musculus
14 Dars 226414
Co-fractionation Mus musculus
15 AI314180 230249
Co-fractionation Mus musculus
16 Atp9a  
Co-fractionation Mus musculus
17 Lars2  
Co-fractionation Mus musculus
18 Chtf18  
Co-fractionation Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Top1 21969
Co-fractionation Mus musculus
21 Atg16l1  
Affinity Capture-MS Mus musculus
22 Abce1 24015
Co-fractionation Mus musculus
23 Rars 104458
Co-fractionation Mus musculus
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