Gene description for Rars
Gene name arginyl-tRNA synthetase
Gene symbol Rars
Other names/aliases 2610011N19Rik
2610037E21Rik
AL033339
AW985894
Species Mus musculus
 Database cross references - Rars
ExoCarta ExoCarta_104458
Vesiclepedia VP_104458
Entrez Gene 104458
UniProt Q9D0I9  
 Rars identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rars
Molecular Function
    tRNA binding GO:0000049 IEA
    tRNA binding GO:0000049 ISO
    arginine-tRNA ligase activity GO:0004814 IBA
    arginine-tRNA ligase activity GO:0004814 IMP
    arginine-tRNA ligase activity GO:0004814 ISO
    arginine-tRNA ligase activity GO:0004814 ISS
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    arginine binding GO:0034618 IEA
    arginine binding GO:0034618 ISO
Biological Process
    arginyl-tRNA aminoacylation GO:0006420 IBA
    arginyl-tRNA aminoacylation GO:0006420 IDA
    arginyl-tRNA aminoacylation GO:0006420 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IDA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 ISO
 Experiment description of studies that identified Rars in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rars
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcna3  
Affinity Capture-MS Mus musculus
2 Dars 226414
Co-fractionation Mus musculus
3 Rpl27 19942
Co-fractionation Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Lars 107045
Co-fractionation Mus musculus
6 Eprs 107508
Co-fractionation Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 Eef1e1  
Co-fractionation Mus musculus
9 Sdcbp 53378
Co-fractionation Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Kars 85305
Co-fractionation Mus musculus
12 Wdr61  
Co-fractionation Mus musculus
13 Nars2 244141
Co-fractionation Mus musculus
14 Aimp2 231872
Co-fractionation Mus musculus
15 Fancd2  
Affinity Capture-MS Mus musculus
16 Aimp1 13722
Co-fractionation Mus musculus
17 VASP 7408
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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