Gene description for CPLX1
Gene name complexin 1
Gene symbol CPLX1
Other names/aliases CPX-I
CPX1
Species Homo sapiens
 Database cross references - CPLX1
ExoCarta ExoCarta_10815
Vesiclepedia VP_10815
Entrez Gene 10815
HGNC 2309
MIM 605032
UniProt O14810  
 CPLX1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for CPLX1
Molecular Function
    SNARE binding GO:0000149 IBA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IEA
Biological Process
    exocytosis GO:0006887 TAS
    chemical synaptic transmission GO:0007268 TAS
    synaptic vesicle exocytosis GO:0016079 IBA
    regulation of exocytosis GO:0017157 TAS
    insulin secretion GO:0030073 IEA
    regulation of synaptic vesicle fusion to presynaptic active zone membrane GO:0031630 IBA
    modulation of chemical synaptic transmission GO:0050804 IBA
    regulation of exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0099145 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    SNARE complex GO:0031201 IBA
    terminal bouton GO:0043195 IBA
    perikaryon GO:0043204 IEA
    calyx of Held GO:0044305 IEA
    synaptobrevin 2-SNAP-25-syntaxin-3-complexin complex GO:0070554 TAS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified CPLX1 in sEVs
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CPLX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIP6 7205
Co-fractionation Homo sapiens
2 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KHSRP 8570
Co-fractionation Homo sapiens
5 HBS1L 10767
Co-fractionation Homo sapiens
6 CMTM5  
Two-hybrid Homo sapiens
7 PRNP 5621
Affinity Capture-MS Homo sapiens
8 NUBP2 10101
Co-fractionation Homo sapiens
9 HSPA8 3312
Affinity Capture-MS Homo sapiens
10 HN1 51155
Co-fractionation Homo sapiens
11 IQCB1  
Affinity Capture-MS Homo sapiens
12 DPY30 84661
Co-fractionation Homo sapiens
13 SNRPA 6626
Co-fractionation Homo sapiens
14 POMP  
Co-fractionation Homo sapiens
15 NENF 29937
Co-fractionation Homo sapiens
16 PPIA 5478
Co-fractionation Homo sapiens
17 VAMP2 6844
Co-crystal Structure Homo sapiens
18 SNAP25 6616
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
19 NDRG3 57446
Co-fractionation Homo sapiens
20 S100A9 6280
Co-fractionation Homo sapiens
21 GINS3  
Co-fractionation Homo sapiens
22 AGTRAP 57085
Two-hybrid Homo sapiens
23 GLO1 2739
Co-fractionation Homo sapiens
24 PFDN5 5204
Co-fractionation Homo sapiens
25 CPLX2 10814
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TAGLN2 8407
Co-fractionation Homo sapiens
27 STX1A 6804
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
28 UBE2B 7320
Co-fractionation Homo sapiens
29 FLNA 2316
Co-fractionation Homo sapiens
30 OPRM1 4988
Two-hybrid Homo sapiens
31 SEC24C 9632
Co-fractionation Homo sapiens
32 GGA2 23062
Co-fractionation Homo sapiens
33 PSMD9 5715
Co-fractionation Homo sapiens
34 SGTA 6449
Co-fractionation Homo sapiens
35 RNF114 55905
Co-fractionation Homo sapiens
36 DBN1 1627
Cross-Linking-MS (XL-MS) Homo sapiens
37 PSMD2 5708
Co-fractionation Homo sapiens
38 STAM 8027
Co-fractionation Homo sapiens
39 UBE2V2 7336
Co-fractionation Homo sapiens
40 NEDD8 4738
Co-fractionation Homo sapiens
41 TACC3  
Co-fractionation Homo sapiens
View the network image/svg+xml



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