Experiment description of studies that identified PWP1 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for PWP1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 RIT1 6016 Negative Genetic Homo sapiens 3 ELF1 1997 Proximity Label-MS Homo sapiens 4 KLF9 687 Proximity Label-MS Homo sapiens 5 LHX2   Proximity Label-MS Homo sapiens 6 SOX17   Proximity Label-MS Homo sapiens 7 EN1   Proximity Label-MS Homo sapiens 8 NOP56 10528 Affinity Capture-MS Homo sapiens 9 RPL15 6138 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 CAND1 55832 Affinity Capture-MS Homo sapiens 11 SOX2   Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 APP 351 Reconstituted Complex Homo sapiens 13 USP36   Affinity Capture-MS Homo sapiens 14 DDA1   Affinity Capture-MS Homo sapiens 15 PRR11   Affinity Capture-MS Homo sapiens 16 KLF8   Proximity Label-MS Homo sapiens 17 DDX24 57062 Co-fractionation Homo sapiens 18 COPS6 10980 Affinity Capture-MS Homo sapiens 19 COPS5 10987 Affinity Capture-MS Homo sapiens 20 KLF6   Proximity Label-MS Homo sapiens 21 NPM1 4869 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 KLF15   Proximity Label-MS Homo sapiens 23 OBSL1 23363 Affinity Capture-MS Homo sapiens 24 DDX18 8886 Co-fractionation Homo sapiens 25 UTP20 27340 Co-fractionation Homo sapiens 26 FBL 2091 Co-fractionation Homo sapiens 27 LHX4   Proximity Label-MS Homo sapiens 28 MECP2 4204 Affinity Capture-MS Homo sapiens 29 CUL1 8454 Affinity Capture-MS Homo sapiens 30 ARIH2 10425 Affinity Capture-MS Homo sapiens 31 WDR5 11091 Affinity Capture-MS Homo sapiens 32 NEDD8 4738 Affinity Capture-MS Homo sapiens 33 RBM8A 9939 Affinity Capture-MS Homo sapiens 34 KIF14 9928 Affinity Capture-MS Homo sapiens 35 PES1 23481 Co-fractionation Homo sapiens 36 FAS 355 Proximity Label-MS Homo sapiens 37 LIN28A   Affinity Capture-MS Homo sapiens 38 KLF16   Proximity Label-MS Homo sapiens 39 UFL1 23376 Affinity Capture-MS Homo sapiens 40 KIAA0020 9933 Co-fractionation Homo sapiens 41 TLX3   Proximity Label-MS Homo sapiens 42 CUL4B 8450 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 43 PARK2   Affinity Capture-MS Homo sapiens 44 DRG1 4733 Affinity Capture-MS Homo sapiens 45 IRF9 10379 Proximity Label-MS Homo sapiens 46 BLM 641 Affinity Capture-MS Homo sapiens 47 ELK3   Proximity Label-MS Homo sapiens 48 HOXA3   Two-hybrid Homo sapiens 49 RPL5 6125 Co-fractionation Homo sapiens 50 LHX1   Proximity Label-MS Homo sapiens 51 Srp72   Affinity Capture-MS Mus musculus 52 LGALS13   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 OGT 8473 Reconstituted Complex Homo sapiens 54 IFI16 3428 Affinity Capture-MS Homo sapiens 55 DDX10   Co-fractionation Homo sapiens 56 TRIM11   Affinity Capture-MS Homo sapiens 57 HECTD1 25831 Affinity Capture-MS Homo sapiens 58 KLF12   Proximity Label-MS Homo sapiens 59 PSG2   Affinity Capture-MS Homo sapiens 60 GTPBP4 23560 Co-fractionation Homo sapiens 61 NFATC4 4776 Proximity Label-MS Homo sapiens 62 ATG16L1 55054 Affinity Capture-MS Homo sapiens 63 TLX2   Proximity Label-MS Homo sapiens 64 HSPA5 3309 Affinity Capture-MS Homo sapiens 65 ZBTB2 57621 Affinity Capture-MS Homo sapiens 66 IPO5 3843 Affinity Capture-MS Homo sapiens 67 RC3H2   Affinity Capture-MS Homo sapiens 68 ECT2 1894 Affinity Capture-MS Homo sapiens 69 SIRT7   Affinity Capture-MS Homo sapiens 70 TSPYL5   Affinity Capture-MS Homo sapiens 71 CUL3 8452 Affinity Capture-MS Homo sapiens 72 NR3C1 2908 Proximity Label-MS Homo sapiens 73 EGFR 1956 Negative Genetic Homo sapiens 74 YY1 7528 Proximity Label-MS Homo sapiens 75 SOX15   Affinity Capture-MS Homo sapiens 76 MYCN   Affinity Capture-MS Homo sapiens 77 MYC   Affinity Capture-MS Homo sapiens 78 TOP1 7150 Co-fractionation Homo sapiens 79 ETV3   Proximity Label-MS Homo sapiens 80 DDB1 1642 Affinity Capture-Western Homo sapiens 81 DDRGK1 65992 Affinity Capture-MS Homo sapiens 82 RNF2   Affinity Capture-MS Homo sapiens 83 ANK3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 84 NFYA 4800 Two-hybrid Homo sapiens 85 FTSJ3 117246 Co-fractionation Homo sapiens 86 HUWE1 10075 Affinity Capture-MS Homo sapiens 87 STAT4   Proximity Label-MS Homo sapiens 88 RBM34   Co-fractionation Homo sapiens 89 SIRT6   Affinity Capture-MS Homo sapiens 90 FBXL6   Affinity Capture-MS Homo sapiens 91 TP53 7157 Affinity Capture-MS Homo sapiens 92 MNDA 4332 Affinity Capture-MS Homo sapiens 93 SRP19 6728 Affinity Capture-MS Homo sapiens 94 SOX4   Proximity Label-MS Homo sapiens 95 SSBP1 6742 Affinity Capture-MS Homo sapiens 96 KLF5   Proximity Label-MS Homo sapiens 97 NCL 4691 Affinity Capture-MS Homo sapiens 98 ASH2L 9070 Affinity Capture-MS Homo sapiens 99 RPL7A 6130 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 100 TCAF1 9747 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 101 CIT 11113 Affinity Capture-MS Homo sapiens 102 KRAS 3845 Negative Genetic Homo sapiens 103 RC3H1 149041 Affinity Capture-MS Homo sapiens 104 SP7   Proximity Label-MS Homo sapiens 105 CUL4A 8451 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 106 EP300 2033 Affinity Capture-MS Homo sapiens View the network

Pathways in which PWP1 is involved

No pathways found