Gene description for UTP20
Gene name UTP20, small subunit (SSU) processome component, homolog (yeast)
Gene symbol UTP20
Other names/aliases DRIM
Species Homo sapiens
 Database cross references - UTP20
ExoCarta ExoCarta_27340
Vesiclepedia VP_27340
Entrez Gene 27340
HGNC 17897
MIM 612822
UniProt O75691  
 UTP20 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for UTP20
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000447 ISS
    endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000472 ISS
    endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000480 ISS
    rRNA processing GO:0006364 IMP
    negative regulation of cell population proliferation GO:0008285 TAS
    ribosomal small subunit biogenesis GO:0042274 IDA
Subcellular Localization
    nucleoplasm GO:0005654 ISS
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IDA
    90S preribosome GO:0030686 IBA
    90S preribosome GO:0030686 ISS
    preribosome, small subunit precursor GO:0030688 ISS
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 IDA
    small-subunit processome GO:0032040 ISS
 Experiment description of studies that identified UTP20 in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UTP20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 VPS41 27072
Co-fractionation Homo sapiens
3 NOP58 51602
Co-fractionation Homo sapiens
4 COIL  
Proximity Label-MS Homo sapiens
5 KPNA1 3836
Affinity Capture-MS Homo sapiens
6 NOP56 10528
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
7 PRPF3  
Co-fractionation Homo sapiens
8 CENPA  
Proximity Label-MS Homo sapiens
9 USP36  
Affinity Capture-MS Homo sapiens
10 DDX23 9416
Proximity Label-MS Homo sapiens
11 Ercc6l  
Affinity Capture-MS Mus musculus
12 PRC1 9055
Affinity Capture-MS Homo sapiens
13 FBL 2091
Proximity Label-MS Homo sapiens
14 POLR1E  
Proximity Label-MS Homo sapiens
15 OBSL1 23363
Affinity Capture-MS Homo sapiens
16 JTB 10899
Affinity Capture-MS Homo sapiens
17 DGCR2 9993
Affinity Capture-MS Homo sapiens
18 DDX18 8886
Co-fractionation Homo sapiens
19 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GPR17 2840
Affinity Capture-MS Homo sapiens
21 KIF14 9928
Affinity Capture-MS Homo sapiens
22 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 EFNA5  
Affinity Capture-MS Homo sapiens
26 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
27 CLSTN1 22883
Affinity Capture-MS Homo sapiens
28 PBXIP1 57326
Affinity Capture-MS Homo sapiens
29 RPL31 6160
Proximity Label-MS Homo sapiens
30 NPTN 27020
Affinity Capture-MS Homo sapiens
31 IFI16 3428
Affinity Capture-MS Homo sapiens
32 GPR182  
Affinity Capture-MS Homo sapiens
33 CLSTN2 64084
Affinity Capture-MS Homo sapiens
34 ZFP91 80829
Co-fractionation Homo sapiens
35 PML 5371
Affinity Capture-MS Homo sapiens
36 HECTD1 25831
Affinity Capture-MS Homo sapiens
37 ITFG3 83986
Affinity Capture-MS Homo sapiens
38 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 VPS18 57617
Co-fractionation Homo sapiens
40 CD3EAP  
Proximity Label-MS Homo sapiens
41 PPP1R26  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 ANLN 54443
Affinity Capture-MS Homo sapiens
43 CYB5B 80777
Affinity Capture-MS Homo sapiens
44 EFNA4  
Affinity Capture-MS Homo sapiens
45 LMNB1 4001
Affinity Capture-MS Homo sapiens
46 ECT2 1894
Affinity Capture-MS Homo sapiens
47 SIRT7  
Affinity Capture-MS Homo sapiens
48 NAT10 55226
Co-fractionation Homo sapiens
49 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RPS6 6194
Proximity Label-MS Homo sapiens
52 DCAF13 25879
Co-fractionation Homo sapiens
53 EGFR 1956
Negative Genetic Homo sapiens
54 FOXL1  
Affinity Capture-MS Homo sapiens
55 TMEM74  
Affinity Capture-MS Homo sapiens
56 OCIAD1 54940
Affinity Capture-MS Homo sapiens
57 C3orf18  
Affinity Capture-MS Homo sapiens
58 MYC  
Affinity Capture-MS Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 ATP2A1 487
Proximity Label-MS Homo sapiens
61 TOP1 7150
Co-fractionation Homo sapiens
62 CD80 941
Affinity Capture-MS Homo sapiens
63 EED  
Affinity Capture-MS Homo sapiens
64 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 UPF3B 65109
Co-fractionation Homo sapiens
66 TNFRSF1B  
Affinity Capture-MS Homo sapiens
67 BMI1  
Affinity Capture-MS Homo sapiens
68 NOL6 65083
Co-fractionation Homo sapiens
69 AKAP1 8165
Proximity Label-MS Homo sapiens
70 DDRGK1 65992
Affinity Capture-MS Homo sapiens
71 CCDC8  
Affinity Capture-MS Homo sapiens
72 GYPA  
Affinity Capture-MS Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 PARP1 142
Proximity Label-MS Homo sapiens
75 RAB3GAP2 25782
Co-fractionation Homo sapiens
76 OPALIN  
Affinity Capture-MS Homo sapiens
77 MNDA 4332
Affinity Capture-MS Homo sapiens
78 MKI67  
Affinity Capture-MS Homo sapiens
79 NIFK 84365
Proximity Label-MS Homo sapiens
80 DHX40  
Proximity Label-MS Homo sapiens
81 PWP1 11137
Co-fractionation Homo sapiens
82 RPS24 6229
Proximity Label-MS Homo sapiens
83 RPS7 6201
Co-fractionation Homo sapiens
84 KRAS 3845
Negative Genetic Homo sapiens
85 CLMP 79827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 ZBTB7A 51341
Co-fractionation Homo sapiens
87 ARSF 416
Affinity Capture-MS Homo sapiens
88 CXCL16 58191
Affinity Capture-MS Homo sapiens
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