Experiment description of studies that identified AKAP11 in sEVs

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for AKAP11

Protein Interactor ExoCarta ID Identification method PubMed Species 1 TRMT1 55621 Affinity Capture-MS Homo sapiens 2 ROPN1   Affinity Capture-MS Homo sapiens 3 SFRP4 6424 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 ERMAP 114625 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 KLHL28   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 FGD5   Affinity Capture-MS Homo sapiens 7 PICK1   Affinity Capture-MS Homo sapiens 8 PRKAR1B   Affinity Capture-MS Homo sapiens 9 FGF11   Affinity Capture-MS Homo sapiens 10 NPAS1   Affinity Capture-MS Homo sapiens 11 TFDP3   Affinity Capture-MS Homo sapiens 12 KIF14 9928 Affinity Capture-MS Homo sapiens 13 VAPB 9217 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 IQCF2   Affinity Capture-MS Homo sapiens 15 VPS28 51160 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 GSK3B 2932 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 17 VAPA 9218 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 DYRK1B   Affinity Capture-MS Homo sapiens 19 UBE2H 7328 Affinity Capture-MS Homo sapiens 20 SEC61B 10952 Proximity Label-MS Homo sapiens 21 MCM5 4174 Affinity Capture-MS Homo sapiens 22 KRT8 3856 Proximity Label-MS Homo sapiens 23 DNA2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 PPP1CA 5499 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 25 PRKAR2A 5576 Protein-peptide Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 26 CDKN2AIP   Affinity Capture-MS Homo sapiens 27 PRKAR1A 5573 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 28 FAM43A   Affinity Capture-MS Homo sapiens 29 PRKACG   Affinity Capture-MS Homo sapiens 30 CUL3 8452 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 ETFB 2109 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 MAP10   Affinity Capture-MS Homo sapiens 33 DCLRE1C   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 TSPYL1 7259 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 TIMP3 7078 Affinity Capture-MS Homo sapiens 36 RABGGTB 5876 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 DUSP16   Affinity Capture-MS Homo sapiens 38 PRKAR2B 5577 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Far Western Homo sapiens Affinity Capture-Western Homo sapiens 39 AP2M1 1173 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 GSK3A 2931 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 41 PPP1CC 5501 Affinity Capture-Western Homo sapiens 42 MLF1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 PRKACA 5566 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 GOLGA6L2   Affinity Capture-MS Homo sapiens 45 CCDC96   Affinity Capture-MS Homo sapiens 46 FGF14   Affinity Capture-MS Homo sapiens 47 MYO6 4646 Proximity Label-MS Homo sapiens 48 SMYD2 56950 Affinity Capture-MS Homo sapiens 49 SEC62 7095 Proximity Label-MS Homo sapiens 50 DYRK1A 1859 Affinity Capture-MS Homo sapiens 51 CREB3L3   Affinity Capture-MS Homo sapiens 52 LECT2   Affinity Capture-MS Homo sapiens 53 HSPB8 26353 Affinity Capture-MS Homo sapiens 54 CRAMP1L 57585 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 RPL28 6158 Affinity Capture-MS Homo sapiens 56 RAB7A 7879 Proximity Label-MS Homo sapiens 57 DNAJC1 64215 Proximity Label-MS Homo sapiens 58 NAPG 8774 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 CHMP4B 128866 Affinity Capture-MS Homo sapiens 60 PPP1CB 5500 Affinity Capture-Western Homo sapiens 61 HAX1   Proximity Label-MS Homo sapiens 62 LRRC59 55379 Proximity Label-MS Homo sapiens 63 SRP14 6727 Affinity Capture-MS Homo sapiens 64 RABL2A 11159 Affinity Capture-MS Homo sapiens 65 PRKACB 5567 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 Prkacb 18749 Affinity Capture-MS Mus musculus 67 PLD1 5337 Affinity Capture-MS Homo sapiens 68 DERL1 79139 Proximity Label-MS Homo sapiens 69 PIGH   Affinity Capture-MS Homo sapiens 70 SULF2 55959 Affinity Capture-MS Homo sapiens View the network

Pathways in which AKAP11 is involved

No pathways found