Gene description for TP53RK
Gene name TP53 regulating kinase
Gene symbol TP53RK
Other names/aliases BUD32
C20orf64
Nori-2
Nori-2p
PRPK
dJ101A2
Species Homo sapiens
 Database cross references - TP53RK
ExoCarta ExoCarta_112858
Vesiclepedia VP_112858
Entrez Gene 112858
HGNC 16197
MIM 608679
UniProt Q96S44  
 TP53RK identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for TP53RK
Molecular Function
    p53 binding GO:0002039 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    tRNA processing GO:0008033 IEA
    tRNA threonylcarbamoyladenosine metabolic process GO:0070525 IBA
    regulation of signal transduction by p53 class mediator GO:1901796 TAS
Subcellular Localization
    EKC/KEOPS complex GO:0000408 IBA
    EKC/KEOPS complex GO:0000408 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
 Experiment description of studies that identified TP53RK in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TP53RK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COMMD10 51397
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MEOX1  
Affinity Capture-MS Homo sapiens
3 INADL 10207
Affinity Capture-MS Homo sapiens
4 ACTR2 10097
Co-fractionation Homo sapiens
5 CPSF6 11052
Affinity Capture-MS Homo sapiens
6 POP7  
Affinity Capture-MS Homo sapiens
7 STX19  
Affinity Capture-MS Homo sapiens
8 MTMR10 54893
Affinity Capture-MS Homo sapiens
9 DPP9 91039
Co-fractionation Homo sapiens
10 WDR4 10785
Co-fractionation Homo sapiens
11 COMMD4 54939
Affinity Capture-MS Homo sapiens
12 NIF3L1 60491
Co-fractionation Homo sapiens
13 Osgep  
Affinity Capture-MS Mus musculus
14 TP53 7157
Reconstituted Complex Homo sapiens
15 UBA5 79876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
17 TPD52L2 7165
Co-fractionation Homo sapiens
18 RIT1 6016
Negative Genetic Homo sapiens
19 SH3GL1 6455
Co-fractionation Homo sapiens
20 CALU 813
Co-fractionation Homo sapiens
21 GORASP2 26003
Two-hybrid Homo sapiens
22 RANGAP1 5905
Co-fractionation Homo sapiens
23 C14orf142  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NSUN2 54888
Co-fractionation Homo sapiens
25 PANK4 55229
Co-fractionation Homo sapiens
26 EFTUD1 79631
Co-fractionation Homo sapiens
27 CYTH4  
Affinity Capture-MS Homo sapiens
28 EGFR 1956
Negative Genetic Homo sapiens
29 OSGEP 55644
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NUP43 348995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SAE1 10055
Co-fractionation Homo sapiens
32 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
33 AARSD1 80755
Co-fractionation Homo sapiens
34 NELL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
36 TOM1L1 10040
Co-fractionation Homo sapiens
37 PRAME  
Affinity Capture-Western Homo sapiens
38 MBIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KIF1BP 26128
Co-fractionation Homo sapiens
40 LAGE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 OGT 8473
Co-fractionation Homo sapiens
43 VARS 7407
Co-fractionation Homo sapiens
44 ARPP19 10776
Co-fractionation Homo sapiens
45 ACTR3 10096
Co-fractionation Homo sapiens
46 UFM1 51569
Co-fractionation Homo sapiens
47 RPRD1B 58490
Co-fractionation Homo sapiens
48 PRKACB 5567
Co-fractionation Homo sapiens
49 GLRX3 10539
Co-fractionation Homo sapiens
50 THG1L 54974
Co-fractionation Homo sapiens
51 TPRKB 51002
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SMS 6611
Affinity Capture-MS Homo sapiens
53 KRAS 3845
Negative Genetic Homo sapiens
54 CALCOCO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 LPPR2  
Affinity Capture-MS Homo sapiens
56 CTH 1491
Co-fractionation Homo sapiens
57 PRPS1 5631
Co-fractionation Homo sapiens
58 MTUS2 23281
Two-hybrid Homo sapiens
59 PAGR1  
Affinity Capture-MS Homo sapiens
60 EEF2K  
Co-fractionation Homo sapiens
61 LURAP1L  
Affinity Capture-MS Homo sapiens
62 ANO4 121601
Affinity Capture-MS Homo sapiens
63 MDM2  
Biochemical Activity Homo sapiens
64 METTL1 4234
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here