Gene description for EXOSC6
Gene name exosome component 6
Gene symbol EXOSC6
Other names/aliases EAP4
MTR3
Mtr3p
hMtr3p
p11
Species Homo sapiens
 Database cross references - EXOSC6
ExoCarta ExoCarta_118460
Vesiclepedia VP_118460
Entrez Gene 118460
HGNC 19055
MIM 606490
UniProt Q5RKV6  
 EXOSC6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for EXOSC6
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA exonuclease activity GO:0004532 IDA
Biological Process
    rRNA processing GO:0006364 IEA
    RNA processing GO:0006396 IDA
    RNA catabolic process GO:0006401 IDA
    rRNA catabolic process GO:0016075 IBA
    U4 snRNA 3'-end processing GO:0034475 IBA
    DNA deamination GO:0045006 IDA
    isotype switching GO:0045190 ISS
    positive regulation of isotype switching GO:0045830 IEA
    nuclear mRNA surveillance GO:0071028 IBA
    poly(A)-dependent snoRNA 3'-end processing GO:0071051 IBA
Subcellular Localization
    nuclear exosome (RNase complex) GO:0000176 IBA
    nuclear exosome (RNase complex) GO:0000176 NAS
    cytoplasmic exosome (RNase complex) GO:0000177 IBA
    cytoplasmic exosome (RNase complex) GO:0000177 NAS
    exosome (RNase complex) GO:0000178 IDA
    exosome (RNase complex) GO:0000178 NAS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nucleolar exosome (RNase complex) GO:0101019 NAS
 Experiment description of studies that identified EXOSC6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EXOSC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PSMA6 5687
Co-fractionation Homo sapiens
3 HECTD1 25831
Affinity Capture-MS Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 EXOSC10 5394
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 EXOSC6 118460
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 NUDT21 11051
Two-hybrid Homo sapiens
8 CHPF 79586
Two-hybrid Homo sapiens
9 EXOSC7 23016
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
11 ARIH2 10425
Affinity Capture-MS Homo sapiens
12 MPHOSPH6 10200
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PSMA3 5684
Co-fractionation Homo sapiens
14 LSM8 51691
Two-hybrid Homo sapiens
15 MIF4GD 57409
Two-hybrid Homo sapiens
16 HDGF 3068
Affinity Capture-MS Homo sapiens
17 LSM1 27257
Two-hybrid Homo sapiens
18 DCP1B  
Two-hybrid Homo sapiens
19 MAN2C1 4123
Two-hybrid Homo sapiens
20 ACOT7 11332
Affinity Capture-MS Homo sapiens
21 NDUFA13 51079
Two-hybrid Homo sapiens
22 EXOSC9 5393
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FAS 355
Proximity Label-MS Homo sapiens
24 MCF2L2 23101
Affinity Capture-MS Homo sapiens
25 RIT1 6016
Negative Genetic Homo sapiens
26 MNDA 4332
Affinity Capture-MS Homo sapiens
27 C1QBP 708
Two-hybrid Homo sapiens
28 TUFM 7284
Two-hybrid Homo sapiens
29 AURKA 6790
Affinity Capture-MS Homo sapiens
30 DIS3L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PHF10  
Affinity Capture-MS Homo sapiens
32 MEX3A  
Affinity Capture-RNA Homo sapiens
33 SOX2  
Affinity Capture-MS Homo sapiens
34 THOP1 7064
Affinity Capture-MS Homo sapiens
35 EFNA1  
Affinity Capture-MS Homo sapiens
36 CUL3 8452
Affinity Capture-MS Homo sapiens
37 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
38 PSMA2 5683
Co-fractionation Homo sapiens
39 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
40 EXOSC1 51013
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
41 ZFP36  
Two-hybrid Homo sapiens
42 SRSF3 6428
Two-hybrid Homo sapiens
43 XRN1 54464
Two-hybrid Homo sapiens
44 RBM7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PSMA1 5682
Co-fractionation Homo sapiens
46 DUSP13  
Affinity Capture-MS Homo sapiens
47 USP36  
Affinity Capture-MS Homo sapiens
48 GIGYF2 26058
Affinity Capture-MS Homo sapiens
49 CEP250 11190
Affinity Capture-MS Homo sapiens
50 SKIV2L2 23517
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
51 LSM7  
Two-hybrid Homo sapiens
52 FBXW7  
Affinity Capture-MS Homo sapiens
53 MRPS18B 28973
Two-hybrid Homo sapiens
54 MRPL4 51073
Two-hybrid Homo sapiens
55 EXOSC2 23404
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 BICD2 23299
Proximity Label-MS Homo sapiens
57 GSE1 23199
Two-hybrid Homo sapiens
58 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
59 ESRRA  
Two-hybrid Homo sapiens
60 EXOSC3 51010
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 EIF3I 8668
Two-hybrid Homo sapiens
63 EXOSC5 56915
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SOD1 6647
Affinity Capture-MS Homo sapiens
65 C1D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
67 DDRGK1 65992
Affinity Capture-MS Homo sapiens
68 RAB3GAP2 25782
Two-hybrid Homo sapiens
69 Rpl35 66489
Affinity Capture-MS Mus musculus
70 PSMA5 5686
Co-fractionation Homo sapiens
71 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 BCL6  
Affinity Capture-MS Homo sapiens
73 DNAJC30  
Two-hybrid Homo sapiens
74 PRMT1 3276
Affinity Capture-MS Homo sapiens
75 UPF2 26019
Two-hybrid Homo sapiens
76 EIF4E2  
Affinity Capture-MS Homo sapiens
77 PSMB1 5689
Two-hybrid Homo sapiens
78 Ksr1  
Affinity Capture-MS Mus musculus
79 MDM2  
Affinity Capture-RNA Homo sapiens
80 Exosc1  
Affinity Capture-MS Mus musculus
81 EXOSC4 54512
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 EXOSC8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
83 MOCS3 27304
Two-hybrid Homo sapiens
84 HBS1L 10767
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
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