Gene description for MIF4GD
Gene name MIF4G domain containing
Gene symbol MIF4GD
Other names/aliases AD023
MIFD
SLIP1
Species Homo sapiens
 Database cross references - MIF4GD
ExoCarta ExoCarta_57409
Vesiclepedia VP_57409
Entrez Gene 57409
HGNC 24030
MIM 612072
UniProt A9UHW6  
 MIF4GD identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MIF4GD
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    translation activator activity GO:0008494 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    cap-dependent translational initiation GO:0002191 IDA
    regulation of translational initiation GO:0006446 IBA
    positive regulation of translation GO:0045727 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 NAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    histone mRNA stem-loop binding complex GO:0062073 IPI
 Experiment description of studies that identified MIF4GD in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MIF4GD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RGL3  
Affinity Capture-MS Homo sapiens
2 ARHGEF5  
Affinity Capture-MS Homo sapiens
3 EXOSC6 118460
Two-hybrid Homo sapiens
4 ENKD1  
Two-hybrid Homo sapiens
5 EIF3G 8666
Two-hybrid Homo sapiens
6 HDAC4  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 HOOK1  
Affinity Capture-MS Homo sapiens
8 UBQLN4 56893
Two-hybrid Homo sapiens
9 SVIL 6840
Two-hybrid Homo sapiens
10 PPIP5K1 9677
Co-fractionation Homo sapiens
11 DDX19B 11269
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 PPARG 5468
Two-hybrid Homo sapiens
13 AQP1 358
Two-hybrid Homo sapiens
14 RICTOR 253260
Affinity Capture-MS Homo sapiens
15 MKRN3  
Two-hybrid Homo sapiens
16 LSM5  
Co-fractionation Homo sapiens
17 DDX19A 55308
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
18 PSMC1 5700
Affinity Capture-MS Homo sapiens
19 ILF2 3608
Co-fractionation Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 HAUS5 23354
Affinity Capture-MS Homo sapiens
22 PDXDC1 23042
Co-fractionation Homo sapiens
23 DHX16 8449
Co-fractionation Homo sapiens
24 EXPH5  
Affinity Capture-MS Homo sapiens
25 CRYBA4  
Two-hybrid Homo sapiens
26 KLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CTIF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 CHCHD2  
Two-hybrid Homo sapiens
29 HPS3 84343
Affinity Capture-MS Homo sapiens
30 ATPAF2 91647
Two-hybrid Homo sapiens
31 FAM110A  
Two-hybrid Homo sapiens
32 PDE4DIP 9659
Two-hybrid Homo sapiens
33 HGS 9146
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 SLBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
35 C7orf60 154743
Affinity Capture-MS Homo sapiens
36 Psmd6 66413
Affinity Capture-MS Mus musculus
37 RTEL1 51750
Affinity Capture-MS Homo sapiens
38 HINFP  
Two-hybrid Homo sapiens
39 C6orf165  
Two-hybrid Homo sapiens
40 DDIAS  
Two-hybrid Homo sapiens
41 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
42 WDYHV1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MIF4GD is involved
No pathways found





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