Gene description for GNPDA2
Gene name glucosamine-6-phosphate deaminase 2
Gene symbol GNPDA2
Other names/aliases GNP2
SB52
Species Homo sapiens
 Database cross references - GNPDA2
ExoCarta ExoCarta_132789
Vesiclepedia VP_132789
Entrez Gene 132789
HGNC 21526
MIM 613222
UniProt Q8TDQ7  
 GNPDA2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Keratinocytes 19530224    
Keratinocytes 19530224    
 Gene ontology annotations for GNPDA2
Molecular Function
    glucosamine-6-phosphate deaminase activity GO:0004342 IBA
    glucosamine-6-phosphate deaminase activity GO:0004342 IDA
    protein binding GO:0005515 IPI
    isomerase activity GO:0016853 IEA
    identical protein binding GO:0042802 IBA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    glucosamine catabolic process GO:0006043 IBA
    N-acetylglucosamine catabolic process GO:0006046 IBA
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 IEA
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 IMP
    N-acetylneuraminate catabolic process GO:0019262 IBA
Subcellular Localization
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GNPDA2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNPDA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DSN1 79980
Negative Genetic Homo sapiens
2 RBMXL1  
Affinity Capture-MS Homo sapiens
3 MAP7 9053
Affinity Capture-MS Homo sapiens
4 SPRY2 10253
Affinity Capture-MS Homo sapiens
5 SOD1 6647
Co-fractionation Homo sapiens
6 C10orf107  
Affinity Capture-MS Homo sapiens
7 C11orf54 28970
Co-fractionation Homo sapiens
8 IGHD  
Affinity Capture-MS Homo sapiens
9 LTBR 4055
Affinity Capture-MS Homo sapiens
10 AMDHD2 51005
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 UBE2L6 9246
Affinity Capture-MS Homo sapiens
12 MTAP 4507
Co-fractionation Homo sapiens
13 DCAF13 25879
Affinity Capture-MS Homo sapiens
14 ADI1 55256
Co-fractionation Homo sapiens
15 PIR 8544
Co-fractionation Homo sapiens
16 PEBP1 5037
Co-fractionation Homo sapiens
17 SHMT1 6470
Co-fractionation Homo sapiens
18 PCBP3 54039
Affinity Capture-MS Homo sapiens
19 LRTM1  
Affinity Capture-MS Homo sapiens
20 PRR11  
Affinity Capture-MS Homo sapiens
21 WDYHV1  
Two-hybrid Homo sapiens
22 SET 6418
Affinity Capture-MS Homo sapiens
23 MAP9 79884
Affinity Capture-MS Homo sapiens
24 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 IFT74 80173
Affinity Capture-MS Homo sapiens
26 MAGOHB 55110
Two-hybrid Homo sapiens
27 UMPS 7372
Co-fractionation Homo sapiens
28 FH 2271
Co-fractionation Homo sapiens
29 SNX12 29934
Co-fractionation Homo sapiens
30 AKR1A1 10327
Co-fractionation Homo sapiens
31 MYH11 4629
Affinity Capture-MS Homo sapiens
32 MARCH5  
Affinity Capture-MS Homo sapiens
33 PTRF 284119
Affinity Capture-MS Homo sapiens
34 NOL3 8996
Co-fractionation Homo sapiens
35 GNPDA1 10007
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ERRFI1 54206
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GNPDA2 is involved
PathwayEvidenceSource
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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