Experiment description of studies that identified ERRFI1 in sEVs

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for ERRFI1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PLEKHA3   Two-hybrid Homo sapiens 2 AXL 558 Affinity Capture-Western Homo sapiens 3 NPC2 10577 Two-hybrid Homo sapiens 4 ADAM5   Two-hybrid Homo sapiens 5 GNPDA2 132789 Two-hybrid Homo sapiens 6 CCT3 7203 Affinity Capture-MS Homo sapiens 7 SHC1 6464 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 DCTN6 10671 Two-hybrid Homo sapiens 9 PHYHIPL   Two-hybrid Homo sapiens 10 YWHAE 7531 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 ACTC1 70 Affinity Capture-MS Homo sapiens 12 GFAP 2670 Affinity Capture-MS Homo sapiens 13 CRHBP   Two-hybrid Homo sapiens 14 MFSD5   Two-hybrid Homo sapiens 15 ATM 472 Affinity Capture-Western Homo sapiens 16 PPIA 5478 Two-hybrid Homo sapiens 17 GTF2H2   Two-hybrid Homo sapiens 18 POTEF 728378 Affinity Capture-MS Homo sapiens 19 ZMAT2   Affinity Capture-MS Homo sapiens 20 POTEE 445582 Affinity Capture-MS Homo sapiens 21 TM2D3   Two-hybrid Homo sapiens 22 COPS5 10987 Two-hybrid Homo sapiens 23 STX8 9482 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 24 ACTG2 72 Affinity Capture-MS Homo sapiens 25 SRC 6714 Biochemical Activity Homo sapiens 26 PSMB6 5694 Two-hybrid Homo sapiens 27 YWHAG 7532 Affinity Capture-MS Homo sapiens 28 PLCG1 5335 Affinity Capture-MS Homo sapiens 29 EFEMP1 2202 Two-hybrid Homo sapiens 30 NEU1 4758 Two-hybrid Homo sapiens 31 YWHAH 7533 Affinity Capture-MS Homo sapiens 32 SFN 2810 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 33 TUBA1A 7846 Affinity Capture-MS Homo sapiens 34 POTEKP 440915 Affinity Capture-MS Homo sapiens 35 NEDD4 4734 Reconstituted Complex Homo sapiens Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens 36 TMEM59 9528 Two-hybrid Homo sapiens 37 HSPA8 3312 Affinity Capture-MS Homo sapiens 38 YWHAB 7529 Two-hybrid Homo sapiens 39 ACTA2 59 Affinity Capture-MS Homo sapiens 40 PDGFRB 5159 Affinity Capture-Western Homo sapiens 41 TMEM165 55858 Two-hybrid Homo sapiens 42 FAM96B 51647 Two-hybrid Homo sapiens 43 WDR61 80349 Two-hybrid Homo sapiens 44 UBASH3B 84959 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 ESRRB   Affinity Capture-MS Homo sapiens 46 TUBA1B 10376 Affinity Capture-MS Homo sapiens 47 PCCA 5095 Two-hybrid Homo sapiens 48 ALK 238 Affinity Capture-Western Homo sapiens 49 YWHAQ 10971 Affinity Capture-MS Homo sapiens 50 ACTB 60 Affinity Capture-MS Homo sapiens 51 TUBA1C 84790 Affinity Capture-MS Homo sapiens 52 HYDIN 54768 Two-hybrid Homo sapiens 53 FYN 2534 Affinity Capture-MS Homo sapiens 54 ACTG1 71 Affinity Capture-MS Homo sapiens 55 ACBD3 64746 Two-hybrid Homo sapiens 56 ERRFI1 54206 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 TUBB 203068 Affinity Capture-MS Homo sapiens 58 LINC00998   Two-hybrid Homo sapiens 59 MASP1 5648 Affinity Capture-MS Homo sapiens 60 XPO1 7514 Affinity Capture-MS Homo sapiens 61 SH3YL1   Two-hybrid Homo sapiens 62 AOX1 316 Two-hybrid Homo sapiens 63 MMP26   Two-hybrid Homo sapiens 64 ACTBL2 345651 Affinity Capture-MS Homo sapiens 65 YWHAZ 7534 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 66 EGFR 1956 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Co-crystal Structure Homo sapiens Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 67 WEE1 7465 Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 68 GAS1 2619 Two-hybrid Homo sapiens 69 CDC42 998 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 70 TC2N 123036 Two-hybrid Homo sapiens 71 HSPA1A 3303 Affinity Capture-MS Homo sapiens 72 ERBB2 2064 Affinity Capture-MS Homo sapiens 73 H2AFX 3014 Affinity Capture-Western Homo sapiens 74 AGPAT4-IT1   Two-hybrid Homo sapiens 75 RAB18 22931 Two-hybrid Homo sapiens 76 RPS27A 6233 Affinity Capture-MS Homo sapiens 77 PUS7 54517 Affinity Capture-MS Homo sapiens 78 GRB2 2885 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 79 ARRDC5   Two-hybrid Homo sapiens 80 KIAA0226L   Two-hybrid Homo sapiens 81 SLC7A13   Two-hybrid Homo sapiens 82 IL2RG   Two-hybrid Homo sapiens 83 ABL1 25 Affinity Capture-Western Homo sapiens 84 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 85 PDCD2 5134 Two-hybrid Homo sapiens 86 PCNA 5111 Two-hybrid Homo sapiens 87 LTK 4058 Affinity Capture-Western Homo sapiens 88 ACTA1 58 Affinity Capture-MS Homo sapiens 89 ZPBP   Two-hybrid Homo sapiens 90 TIMP4   Two-hybrid Homo sapiens 91 PSMA7 5688 Two-hybrid Homo sapiens 92 PAPSS1 9061 Two-hybrid Homo sapiens 93 OSTF1 26578 Two-hybrid Homo sapiens 94 ANXA2 302 Affinity Capture-MS Homo sapiens 95 SHC2 25759 Affinity Capture-MS Homo sapiens View the network

Pathways in which ERRFI1 is involved

No pathways found