Gene description for Gnai3
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting 3
Gene symbol Gnai3
Other names/aliases AI158965
AW537698
Galphai3
Gnai-3
Species Mus musculus
 Database cross references - Gnai3
ExoCarta ExoCarta_14679
Vesiclepedia VP_14679
Entrez Gene 14679
UniProt Q9DC51  
 Gnai3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gnai3
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    protein domain specific binding GO:0019904 ISO
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    G protein-coupled serotonin receptor binding GO:0031821 ISO
    GTPase activating protein binding GO:0032794 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    vesicle fusion GO:0006906 ISO
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 ISO
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 ISS
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IBA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 ISO
    regulation of heart contraction GO:0008016 IMP
    positive regulation of macroautophagy GO:0016239 IEA
    positive regulation of macroautophagy GO:0016239 ISO
    positive regulation of superoxide anion generation GO:0032930 ISO
    GTP metabolic process GO:0046039 ISO
    GTP metabolic process GO:0046039 ISS
    cell division GO:0051301 ISO
    cell division GO:0051301 ISS
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    Golgi apparatus GO:0005794 IDA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    zymogen granule GO:0042588 ISO
 Experiment description of studies that identified Gnai3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gnai3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ralbp1  
Co-fractionation Mus musculus
2 Prickle2  
Co-fractionation Mus musculus
3 Syne2  
Co-fractionation Mus musculus
4 Rgs18  
Affinity Capture-Western Mus musculus
5 Rgs12  
Co-fractionation Mus musculus
6 Chn2  
Co-fractionation Mus musculus
7 Cyp2a12  
Co-fractionation Mus musculus
8 Strip2  
Co-fractionation Mus musculus
9 Ncf2  
Co-fractionation Mus musculus
10 Srgap1  
Co-fractionation Mus musculus
11 Arhgap35  
Co-fractionation Mus musculus
12 Atg16l1  
Affinity Capture-MS Mus musculus
13 Rgs14  
Co-fractionation Mus musculus
14 Srgap2 14270
Co-fractionation Mus musculus
15 Fancd2  
Affinity Capture-MS Mus musculus
16 Gnas 14683
Co-fractionation Mus musculus
17 Pdlim5 56376
Co-fractionation Mus musculus
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