Gene description for Srgap2
Gene name SLIT-ROBO Rho GTPase activating protein 2
Gene symbol Srgap2
Other names/aliases 9930124L22Rik
AI448945
FBP2
Fnbp2
srGAP3
Species Mus musculus
 Database cross references - Srgap2
ExoCarta ExoCarta_14270
Vesiclepedia VP_14270
Entrez Gene 14270
UniProt Q91Z67  
 Srgap2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srgap2
Molecular Function
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 ISO
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IDA
    small GTPase binding GO:0031267 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 ISO
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    signal transduction GO:0007165 IEA
    extension of a leading process involved in cell motility in cerebral cortex radial glia guided migration GO:0021816 IMP
    regulation of cell migration GO:0030334 IDA
    negative regulation of cell migration GO:0030336 IBA
    positive regulation of GTPase activity GO:0043547 IDA
    filopodium assembly GO:0046847 IDA
    neuron projection morphogenesis GO:0048812 IMP
    actin filament severing GO:0051014 ISS
    regulation of synapse assembly GO:0051963 IDA
    regulation of synapse assembly GO:0051963 IMP
    dendritic spine development GO:0060996 IMP
    excitatory synapse assembly GO:1904861 IMP
    inhibitory synapse assembly GO:1904862 IMP
    negative regulation of neuron migration GO:2001223 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrial inner membrane GO:0005743 HDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 IDA
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    dendritic spine GO:0043197 IDA
    dendritic spine head GO:0044327 ISO
    dendritic spine head GO:0044327 ISS
    postsynaptic membrane GO:0045211 IDA
    phagocytic vesicle GO:0045335 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Srgap2 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srgap2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ralbp1  
Co-fractionation Mus musculus
2 Chn2  
Co-fractionation Mus musculus
3 Tomm70a  
Co-fractionation Mus musculus
4 Timm44  
Co-fractionation Mus musculus
5 Srgap1  
Co-fractionation Mus musculus
6 Rapgef4  
Co-fractionation Mus musculus
7 Ivns1abp  
Co-fractionation Mus musculus
8 Gnal  
Co-fractionation Mus musculus
9 CDC42 998
FRET Homo sapiens
10 Vav1 22324
Co-fractionation Mus musculus
11 Lnx2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
12 Arhgap15  
Co-fractionation Mus musculus
13 Prickle2  
Co-fractionation Mus musculus
14 Sorbs2  
Co-fractionation Mus musculus
15 Syngap1  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
17 Prickle1 106042
Co-fractionation Mus musculus
18 Gli2  
Co-fractionation Mus musculus
19 Arhgap21  
Co-fractionation Mus musculus
20 Arhgap19  
Co-fractionation Mus musculus
21 Gli1  
Co-fractionation Mus musculus
22 Slc26a7  
Co-fractionation Mus musculus
23 Spopl  
Co-fractionation Mus musculus
24 Ywhae 22627
Affinity Capture-MS Mus musculus
25 Dnmbp  
Co-fractionation Mus musculus
26 Lhx5  
Co-fractionation Mus musculus
27 Slmap  
Co-fractionation Mus musculus
28 Herc2  
Affinity Capture-MS Mus musculus
29 Dock1  
Co-fractionation Mus musculus
30 Arhgap42  
Co-fractionation Mus musculus
31 Cyp2a12  
Co-fractionation Mus musculus
32 Gnai3 14679
Co-fractionation Mus musculus
33 Fgd4  
Co-fractionation Mus musculus
34 Syne2  
Co-fractionation Mus musculus
35 Rasgef1b  
Co-fractionation Mus musculus
36 Slc7a2  
Co-fractionation Mus musculus
37 Stard13  
Co-fractionation Mus musculus
38 RAC1 5879
FRET Homo sapiens
39 Gnas 14683
Co-fractionation Mus musculus
40 Arhgap26  
Co-fractionation Mus musculus
41 Lnx1  
Affinity Capture-Western Mus musculus
42 SCOC 60592
Affinity Capture-MS Homo sapiens
43 Arhgap35  
Co-fractionation Mus musculus
44 Arhgap40  
Co-fractionation Mus musculus
45 Pdlim5 56376
Co-fractionation Mus musculus
46 Sorbs1  
Co-fractionation Mus musculus
47 Lpxn  
Co-fractionation Mus musculus
48 KPRP 448834
Affinity Capture-MS Homo sapiens
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