Gene description for Vav1
Gene name vav 1 oncogene
Gene symbol Vav1
Other names/aliases Vav
vav-T
Species Mus musculus
 Database cross references - Vav1
ExoCarta ExoCarta_22324
Vesiclepedia VP_22324
Entrez Gene 22324
UniProt P27870  
 Vav1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vav1
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 EXP
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IGI
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    phosphorylation-dependent protein binding GO:0140031 IPI
Biological Process
    immune response-regulating cell surface receptor signaling pathway GO:0002768 IBA
    phagocytosis GO:0006909 IGI
    immune response GO:0006955 IMP
    G protein-coupled receptor signaling pathway GO:0007186 IGI
    integrin-mediated signaling pathway GO:0007229 IGI
    small GTPase-mediated signal transduction GO:0007264 IBA
    regulation of cell size GO:0008361 IEA
    regulation of cell size GO:0008361 ISO
    cell migration GO:0016477 IBA
    natural killer cell activation GO:0030101 IEA
    T cell differentiation GO:0030217 IGI
    T cell differentiation GO:0030217 TAS
    neutrophil chemotaxis GO:0030593 IGI
    T cell costimulation GO:0031295 IEA
    intracellular signal transduction GO:0035556 IMP
    T cell activation GO:0042110 IMP
    natural killer cell mediated cytotoxicity GO:0042267 IEA
    natural killer cell mediated cytotoxicity GO:0042267 ISO
    regulation of GTPase activity GO:0043087 IGI
    regulation of GTPase activity GO:0043087 ISO
    positive regulation of GTPase activity GO:0043547 IGI
    positive regulation of cell adhesion GO:0045785 IGI
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 IGI
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IBA
    reactive oxygen species metabolic process GO:0072593 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    cell-cell junction GO:0005911 IDA
 Experiment description of studies that identified Vav1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vav1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Chn2  
Co-fractionation Mus musculus
2 Srgap1  
Co-fractionation Mus musculus
3 Itk  
Synthetic Growth Defect Mus musculus
4 Arhgap15  
Co-fractionation Mus musculus
5 Prickle2  
Co-fractionation Mus musculus
6 Sorbs2  
Co-fractionation Mus musculus
7 Foxp3  
Affinity Capture-MS Mus musculus
8 Prickle1 106042
Co-fractionation Mus musculus
9 Kit  
Two-hybrid Mus musculus
10 Gli2  
Co-fractionation Mus musculus
11 Gli1  
Co-fractionation Mus musculus
12 Nck2 17974
Co-fractionation Mus musculus
13 Fgd4  
Co-fractionation Mus musculus
14 Runx2  
Affinity Capture-MS Mus musculus
15 Arhgap21  
Co-fractionation Mus musculus
16 Arhgap26  
Co-fractionation Mus musculus
17 Fancd2  
Affinity Capture-MS Mus musculus
18 Srgap2 14270
Co-fractionation Mus musculus
19 Arhgef7  
Co-fractionation Mus musculus
20 Zap70 22637
Affinity Capture-Western Mus musculus
21 Atg16l1  
Affinity Capture-MS Mus musculus
22 Sorbs1  
Co-fractionation Mus musculus
23 Sorbs3  
Co-fractionation Mus musculus
24 Socs1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Vav1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers IEA Reactome
Azathioprine ADME IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
Cell death signalling via NRAGE, NRIF and NADE IEA Reactome
Costimulation by the CD28 family IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Death Receptor Signaling IEA Reactome
Drug ADME IEA Reactome
Erythropoietin activates RAS IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated Ca+2 mobilization IEA Reactome
FCERI mediated MAPK activation IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
G alpha (12/13) signalling events IEA Reactome
GPCR downstream signalling IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
NRAGE signals death through JNK IEA Reactome
p75 NTR receptor-mediated signalling IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
RAC1 GTPase cycle IEA Reactome
RAC2 GTPase cycle IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Regulation of signaling by CBL IEA Reactome
RHO GTPase cycle IEA Reactome
RHOA GTPase cycle IEA Reactome
RHOG GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by Erythropoietin IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by VEGF IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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