Gene description for Prickle1
Gene name prickle homolog 1 (Drosophila)
Gene symbol Prickle1
Other names/aliases 1110058P22Rik
AW215793
Prickle
b2b019Clo
mpk1
Species Mus musculus
 Database cross references - Prickle1
ExoCarta ExoCarta_106042
Vesiclepedia VP_106042
Entrez Gene 106042
UniProt Q3U5C7  
 Prickle1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prickle1
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    protein-containing complex binding GO:0044877 IDA
Biological Process
    neural tube closure GO:0001843 IMP
    neural tube closure GO:0001843 ISO
    tissue homeostasis GO:0001894 IMP
    outflow tract morphogenesis GO:0003151 IMP
    protein import into nucleus GO:0006606 IEA
    protein import into nucleus GO:0006606 ISO
    apoptotic process GO:0006915 IMP
    cytoskeleton organization GO:0007010 IMP
    establishment or maintenance of cell polarity GO:0007163 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 IMP
    axonogenesis GO:0007409 IMP
    heart development GO:0007507 IMP
    response to xenobiotic stimulus GO:0009410 IMP
    animal organ morphogenesis GO:0009887 IMP
    tissue development GO:0009888 IMP
    gene expression GO:0010467 IMP
    programmed cell death GO:0012501 IMP
    cellular component organization GO:0016043 IMP
    Wnt signaling pathway GO:0016055 IMP
    vesicle-mediated transport GO:0016192 IMP
    dendrite development GO:0016358 IMP
    cell migration GO:0016477 IGI
    cell migration GO:0016477 IMP
    establishment of cell polarity GO:0030010 IGI
    establishment of cell polarity GO:0030010 IMP
    bone mineralization GO:0030282 IMP
    embryonic limb morphogenesis GO:0030326 IMP
    cytoskeleton-dependent intracellular transport GO:0030705 IMP
    neuron projection development GO:0031175 IMP
    positive regulation of protein ubiquitination GO:0031398 IEA
    positive regulation of protein ubiquitination GO:0031398 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    limb morphogenesis GO:0035108 IMP
    multicellular organism growth GO:0035264 IMP
    embryonic nail plate morphogenesis GO:0035880 IMP
    aorta development GO:0035904 IMP
    post-anal tail morphogenesis GO:0036342 IMP
    embryonic digit morphogenesis GO:0042733 IMP
    cilium organization GO:0044782 IMP
    establishment of protein localization GO:0045184 IMP
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    neuron projection morphogenesis GO:0048812 IMP
    response to electrical stimulus GO:0051602 IMP
    cardiac muscle cell development GO:0055013 IMP
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 IMP
    limb development GO:0060173 IMP
    cilium assembly GO:0060271 IMP
    face morphogenesis GO:0060325 IMP
    mesenchyme development GO:0060485 IMP
    coronary vasculature development GO:0060976 IMP
    eyelid development in camera-type eye GO:0061029 IMP
    establishment of bipolar cell polarity involved in cell morphogenesis GO:0061159 IMP
    cornea development in camera-type eye GO:0061303 IMP
    renal tubule development GO:0061326 IMP
    polarized secretion of basement membrane proteins in epithelium GO:0061865 IMP
    tear secretion GO:0070075 IMP
    basement membrane organization GO:0071711 IMP
    extracellular matrix assembly GO:0085029 IMP
    primitive streak formation GO:0090009 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISO
    mitotic spindle assembly GO:0090307 IMP
    cell-cell adhesion GO:0098609 IMP
    regulation of postsynaptic density assembly GO:0099151 IDA
    regulation of postsynaptic density assembly GO:0099151 IMP
    maintenance of postsynaptic density structure GO:0099562 IDA
    maintenance of postsynaptic density structure GO:0099562 IMP
    focal adhesion disassembly GO:0120181 IMP
    anterior visceral endoderm cell migration GO:1905070 IMP
    neuron projection extension GO:1990138 IMP
    embryonic brain development GO:1990403 IMP
    negative regulation of cardiac muscle cell myoblast differentiation GO:2000691 IEA
    negative regulation of cardiac muscle cell myoblast differentiation GO:2000691 ISO
Subcellular Localization
    proteasome complex GO:0000502 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    postsynaptic density GO:0014069 IDA
    membrane GO:0016020 IMP
    cell trailing edge GO:0031254 IDA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Prickle1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prickle1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ralbp1  
Co-fractionation Mus musculus
2 Eps8  
Co-fractionation Mus musculus
3 Chn2  
Co-fractionation Mus musculus
4 Timm44  
Co-fractionation Mus musculus
5 Srgap1  
Co-fractionation Mus musculus
6 Suv420h1  
Co-fractionation Mus musculus
7 Dab2ip  
Co-fractionation Mus musculus
8 Ivns1abp  
Co-fractionation Mus musculus
9 Gnal  
Co-fractionation Mus musculus
10 Syngap1  
Co-fractionation Mus musculus
11 Klhl7  
Co-fractionation Mus musculus
12 Vav1 22324
Co-fractionation Mus musculus
13 Prickle2  
Co-fractionation Mus musculus
14 Arhgap15  
Co-fractionation Mus musculus
15 Fcho2  
Co-fractionation Mus musculus
16 Syne2  
Co-fractionation Mus musculus
17 Cyp2a12  
Co-fractionation Mus musculus
18 Strip2  
Co-fractionation Mus musculus
19 Lman2l  
Co-fractionation Mus musculus
20 Gli2  
Co-fractionation Mus musculus
21 Exoc8  
Co-fractionation Mus musculus
22 Gnas 14683
Co-fractionation Mus musculus
23 Arhgap42  
Co-fractionation Mus musculus
24 Gli1  
Co-fractionation Mus musculus
25 Lhx5  
Co-fractionation Mus musculus
26 Arhgap26  
Co-fractionation Mus musculus
27 Dock1  
Co-fractionation Mus musculus
28 Spopl  
Co-fractionation Mus musculus
29 Osbpl10  
Co-fractionation Mus musculus
30 Dvl1  
Affinity Capture-Western Mus musculus
Phenotypic Suppression Mus musculus
31 Fgd4  
Co-fractionation Mus musculus
32 Pias3  
Co-fractionation Mus musculus
33 Stard13  
Co-fractionation Mus musculus
34 Arhgap21  
Co-fractionation Mus musculus
35 Arhgef7  
Co-fractionation Mus musculus
36 Lhx3  
Co-fractionation Mus musculus
37 Srgap2 14270
Co-fractionation Mus musculus
38 Arhgef2  
Co-fractionation Mus musculus
39 Arhgap35  
Co-fractionation Mus musculus
40 Pdlim5 56376
Co-fractionation Mus musculus
41 Sorbs1  
Co-fractionation Mus musculus
42 Lpxn  
Co-fractionation Mus musculus
43 Sos1  
Co-fractionation Mus musculus
44 Dmd  
Co-fractionation Mus musculus
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