Gene description for NFXL1
Gene name nuclear transcription factor, X-box binding-like 1
Gene symbol NFXL1
Other names/aliases CDZFP
HOZFP
OZFP
URCC5
Species Homo sapiens
 Database cross references - NFXL1
ExoCarta ExoCarta_152518
Vesiclepedia VP_152518
Entrez Gene 152518
HGNC 18726
UniProt Q6ZNB6  
 NFXL1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NFXL1
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    zinc ion binding GO:0008270 IEA
Biological Process
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified NFXL1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NFXL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA4A 7277
Affinity Capture-MS Homo sapiens
2 TUBB 203068
Affinity Capture-MS Homo sapiens
3 TUBB2A 7280
Affinity Capture-MS Homo sapiens
4 RAMP3  
Affinity Capture-MS Homo sapiens
5 FBXW7  
Affinity Capture-MS Homo sapiens
6 SEC63 11231
Proximity Label-MS Homo sapiens
7 PRTN3 5657
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 NAAA  
Affinity Capture-MS Homo sapiens
10 CLINT1 9685
Co-fractionation Homo sapiens
11 WDFY2  
Affinity Capture-MS Homo sapiens
12 AIFM1 9131
Affinity Capture-MS Homo sapiens
13 DHDH  
Affinity Capture-MS Homo sapiens
14 FBXL6  
Affinity Capture-MS Homo sapiens
15 TP53 7157
Negative Genetic Homo sapiens
16 LRRC59 55379
Proximity Label-MS Homo sapiens
17 NUBP2 10101
Affinity Capture-MS Homo sapiens
18 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
19 MFSD4  
Affinity Capture-MS Homo sapiens
20 LRRC25  
Affinity Capture-MS Homo sapiens
21 BSG 682
Affinity Capture-MS Homo sapiens
22 AURKA 6790
Reconstituted Complex Homo sapiens
23 ARSA 410
Affinity Capture-MS Homo sapiens
24 SLC25A22  
Affinity Capture-MS Homo sapiens
25 ASIC4  
Affinity Capture-MS Homo sapiens
26 MGARP  
Affinity Capture-MS Homo sapiens
27 HSD3B7 80270
Proximity Label-MS Homo sapiens
28 CUL3 8452
Affinity Capture-MS Homo sapiens
29 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
30 TUBB4B 10383
Affinity Capture-MS Homo sapiens
31 EMD 2010
Proximity Label-MS Homo sapiens
32 KLRD1  
Affinity Capture-MS Homo sapiens
33 RPN2 6185
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 FOXL1  
Affinity Capture-MS Homo sapiens
36 TUBB3 10381
Affinity Capture-MS Homo sapiens
37 STX4 6810
Proximity Label-MS Homo sapiens
38 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
39 RIPK4  
Affinity Capture-MS Homo sapiens
40 COL20A1  
Cross-Linking-MS (XL-MS) Homo sapiens
41 ALPI  
Affinity Capture-MS Homo sapiens
42 CELA3A  
Affinity Capture-MS Homo sapiens
43 HYAL1 3373
Affinity Capture-MS Homo sapiens
44 SARM1 23098
Affinity Capture-MS Homo sapiens
45 RCCD1  
Affinity Capture-MS Homo sapiens
46 TUBA1C 84790
Affinity Capture-MS Homo sapiens
47 SEC62 7095
Proximity Label-MS Homo sapiens
48 FZD7 8324
Affinity Capture-MS Homo sapiens
49 GPR45  
Affinity Capture-MS Homo sapiens
50 ALDH3B2  
Affinity Capture-MS Homo sapiens
51 CARKD 55739
Affinity Capture-MS Homo sapiens
52 GPR182  
Affinity Capture-MS Homo sapiens
53 NCEH1 57552
Affinity Capture-MS Homo sapiens
54 LPIN3 64900
Affinity Capture-MS Homo sapiens
55 CDK20  
Affinity Capture-MS Homo sapiens
56 RPN1 6184
Affinity Capture-MS Homo sapiens
57 GLMP 112770
Affinity Capture-MS Homo sapiens
58 DDRGK1 65992
Affinity Capture-MS Homo sapiens
59 RNF2  
Affinity Capture-MS Homo sapiens
60 MAGEA9  
Affinity Capture-MS Homo sapiens
61 NAGA 4668
Affinity Capture-MS Homo sapiens
62 CKAP4 10970
Proximity Label-MS Homo sapiens
63 HSPA12A 259217
Affinity Capture-MS Homo sapiens
64 DDOST 1650
Affinity Capture-MS Homo sapiens
65 CANX 821
Affinity Capture-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 FFAR1  
Affinity Capture-MS Homo sapiens
68 C9orf72  
Affinity Capture-MS Homo sapiens
69 SEC61B 10952
Proximity Label-MS Homo sapiens
70 ILF3 3609
Co-fractionation Homo sapiens
71 OST4  
Affinity Capture-MS Homo sapiens
72 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NFXL1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here