Gene description for DSC1
Gene name desmocollin 1
Gene symbol DSC1
Other names/aliases CDHF1
DG2/DG3
Species Homo sapiens
 Database cross references - DSC1
ExoCarta ExoCarta_1823
Vesiclepedia VP_1823
Entrez Gene 1823
HGNC 3035
MIM 125643
UniProt Q08554  
 DSC1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for DSC1
Molecular Function
    calcium ion binding GO:0005509 IBA
    protein binding GO:0005515 IPI
Biological Process
    desmosome maintenance GO:0002160 ISS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    hair follicle morphogenesis GO:0031069 ISS
    negative regulation of epithelial cell proliferation GO:0050680 ISS
    establishment of skin barrier GO:0061436 ISS
    cell-cell adhesion GO:0098609 IBA
Subcellular Localization
    cornified envelope GO:0001533 TAS
    plasma membrane GO:0005886 TAS
    gap junction GO:0005921 TAS
    membrane GO:0016020 TAS
    desmosome GO:0030057 IBA
    extracellular exosome GO:0070062 HDA
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified DSC1 in sEVs
1
Experiment ID 494
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DSC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CEP57  
Affinity Capture-MS Homo sapiens
3 METTL14  
Affinity Capture-MS Homo sapiens
4 CHMP4C 92421
Affinity Capture-MS Homo sapiens
5 SERPINB12 89777
Affinity Capture-MS Homo sapiens
6 CASP14 23581
Affinity Capture-MS Homo sapiens
7 KIF23 9493
Affinity Capture-MS Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 CDC20 991
Affinity Capture-MS Homo sapiens
10 SLX4  
Affinity Capture-MS Homo sapiens
11 C18orf21  
Affinity Capture-MS Homo sapiens
12 FANCD2  
Affinity Capture-MS Homo sapiens
13 JUP 3728
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
14 NEIL3  
Protein-RNA Homo sapiens
15 TP53 7157
Affinity Capture-MS Homo sapiens
16 TBC1D4  
Affinity Capture-MS Homo sapiens
17 C1orf68 100129271
Affinity Capture-MS Homo sapiens
18 TUBG1 7283
Affinity Capture-MS Homo sapiens
19 PKP2 5318
Two-hybrid Homo sapiens
20 TGM3 7053
Affinity Capture-MS Homo sapiens
21 TARDBP 23435
Affinity Capture-MS Homo sapiens
22 Kifc1  
Affinity Capture-MS Mus musculus
23 CRK 1398
Affinity Capture-MS Homo sapiens
24 DLD 1738
Affinity Capture-MS Homo sapiens
25 USP1 7398
Affinity Capture-MS Homo sapiens
26 CD274 29126
Affinity Capture-MS Homo sapiens
27 LAMB1 3912
Affinity Capture-MS Homo sapiens
28 CDSN 1041
Affinity Capture-MS Homo sapiens
29 DSG1 1828
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
30 MCM5 4174
Affinity Capture-MS Homo sapiens
31 ATG101  
Affinity Capture-MS Homo sapiens
32 SLCO4C1 353189
Affinity Capture-MS Homo sapiens
33 ZC3HC1  
Affinity Capture-MS Homo sapiens
34 GOSR1 9527
Affinity Capture-MS Homo sapiens
35 Ddb1 13194
Affinity Capture-MS Mus musculus
36 ZDHHC19  
Affinity Capture-MS Homo sapiens
37 METTL3  
Affinity Capture-MS Homo sapiens
38 AZGP1 563
Affinity Capture-MS Homo sapiens
39 PKP1 5317
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
40 S100A14 57402
Affinity Capture-MS Homo sapiens
41 CDK2 1017
Affinity Capture-MS Homo sapiens
42 ARG1 383
Affinity Capture-MS Homo sapiens
43 MAPK7  
Affinity Capture-MS Homo sapiens
44 UBASH3B 84959
Affinity Capture-MS Homo sapiens
45 GSDMA 284110
Affinity Capture-MS Homo sapiens
46 MYC  
Affinity Capture-MS Homo sapiens
47 XRCC6 2547
Proximity Label-MS Homo sapiens
48 DDX3X 1654
Affinity Capture-MS Homo sapiens
49 TRIM21 6737
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 MCM2 4171
Affinity Capture-MS Homo sapiens
52 NF2 4771
Affinity Capture-MS Homo sapiens
53 PINK1  
Affinity Capture-MS Homo sapiens
54 FABP5 2171
Affinity Capture-MS Homo sapiens
55 BLMH 642
Affinity Capture-MS Homo sapiens
56 PLK1 5347
Affinity Capture-MS Homo sapiens
57 ZUFSP 221302
Affinity Capture-MS Homo sapiens
58 KPRP 448834
Affinity Capture-MS Homo sapiens
59 C9orf72  
Affinity Capture-MS Homo sapiens
60 SERBP1 26135
Affinity Capture-MS Homo sapiens
61 ANXA2 302
Affinity Capture-MS Homo sapiens
62 CEP76  
Affinity Capture-MS Homo sapiens
63 GRK7  
Affinity Capture-MS Homo sapiens
64 DDX39B 7919
Affinity Capture-MS Homo sapiens
65 PRKAB1 5564
Affinity Capture-MS Homo sapiens
66 DSP 1832
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
67 UBXN1 51035
Affinity Capture-MS Homo sapiens
68 CYLD  
Affinity Capture-MS Homo sapiens
69 CUL4A 8451
Affinity Capture-MS Homo sapiens
70 FLG2 388698
Affinity Capture-MS Homo sapiens
71 DSG2 1829
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
72 PKP3 11187
Two-hybrid Homo sapiens
73 UCHL5 51377
Reconstituted Complex Homo sapiens
74 PMVK 10654
Affinity Capture-MS Homo sapiens
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