Gene description for Psmc3
Gene name proteasome (prosome, macropain) 26S subunit, ATPase 3
Gene symbol Psmc3
Other names/aliases TBP-1
Species Mus musculus
 Database cross references - Psmc3
ExoCarta ExoCarta_19182
Vesiclepedia VP_19182
Entrez Gene 19182
UniProt O88685  
 Psmc3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Psmc3
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    proteasome-activating activity GO:0036402 IBA
    identical protein binding GO:0042802 ISO
Biological Process
    blastocyst development GO:0001824 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    proteasome complex GO:0000502 ISO
    P-body GO:0000932 IDA
    nucleus GO:0005634 IEA
    proteasome regulatory particle, base subcomplex GO:0008540 IBA
    proteasome accessory complex GO:0022624 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Psmc3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Psmc3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Usp14 59025
Co-fractionation Mus musculus
2 Psmd8 57296
Co-fractionation Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
4 Psme2 19188
Co-fractionation Mus musculus
5 Psmd2 21762
Co-fractionation Mus musculus
6 Psmb5 19173
Co-fractionation Mus musculus
7 Psmd13 23997
Co-fractionation Mus musculus
8 Uchl5 56207
Co-fractionation Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Txnl1 53382
Co-fractionation Mus musculus
12 Vcp 269523
Co-fractionation Mus musculus
13 Psmc5 19184
Co-fractionation Mus musculus
14 2700029M09Rik  
Co-fractionation Mus musculus
15 Smad5  
Affinity Capture-MS Mus musculus
16 Psmd9  
Co-fractionation Mus musculus
17 Mettl21e  
Affinity Capture-MS Mus musculus
18 Psmd5 66998
Co-fractionation Mus musculus
19 Tmem173  
Proximity Label-MS Mus musculus
20 Psmd7 17463
Co-fractionation Mus musculus
21 3110002H16Rik  
Co-fractionation Mus musculus
22 Smad9  
Affinity Capture-MS Mus musculus
23 Twist1  
Proximity Label-MS Mus musculus
24 Psmd3 22123
Co-fractionation Mus musculus
25 Ino80d  
Co-fractionation Mus musculus
26 Mxi1  
Co-fractionation Mus musculus
27 Psmd6 66413
Co-fractionation Mus musculus
28 Smad1  
Affinity Capture-MS Mus musculus
29 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Psmc3 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport IEA Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Activation of NF-kappaB in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing-Cross presentation IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Assembly of the pre-replicative complex IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Autodegradation of the E3 ubiquitin ligase COP1 IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular response to hypoxia IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Cyclin A:Cdk2-associated events at S phase entry IEA Reactome
Cyclin E associated events during G1/S transition IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
Degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Deubiquitination IEA Reactome
DNA Replication IEA Reactome
DNA Replication Pre-Initiation IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream TCR signaling IEA Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G1/S DNA Damage Checkpoints IEA Reactome
G1/S Transition IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 IEA Reactome
Hedgehog 'off' state IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
KEAP1-NFE2L2 pathway IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of polyamines IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Neddylation IEA Reactome
Neutrophil degranulation IEA Reactome
NIK-->noncanonical NF-kB signaling IEA Reactome
Nuclear events mediated by NFE2L2 IEA Reactome
Orc1 removal from chromatin IEA Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha IEA Reactome
p53-Dependent G1 DNA Damage Response IEA Reactome
p53-Dependent G1/S DNA damage checkpoint IEA Reactome
p53-Independent DNA Damage Response IEA Reactome
p53-Independent G1/S DNA damage checkpoint IEA Reactome
PCP/CE pathway IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome
PTEN Regulation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of RAS by GAPs IEA Reactome
Regulation of RUNX2 expression and activity IEA Reactome
Regulation of RUNX3 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs IEA Reactome
S Phase IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by WNT IEA Reactome
Stabilization of p53 IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
TCR signaling IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
TNFR2 non-canonical NF-kB pathway IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transcriptional regulation by RUNX3 IEA Reactome
Transport of small molecules IEA Reactome
Ub-specific processing proteases IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D IEA Reactome
UCH proteinases IEA Reactome





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