Gene description for Psmd2
Gene name proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Gene symbol Psmd2
Other names/aliases 9430095H01Rik
AA407121
TEG-190
Tex190
Species Mus musculus
 Database cross references - Psmd2
ExoCarta ExoCarta_21762
Vesiclepedia VP_21762
Entrez Gene 21762
UniProt Q8VDM4  
 Psmd2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Psmd2
Molecular Function
    protein binding GO:0005515 IPI
    enzyme regulator activity GO:0030234 IEA
Biological Process
    regulation of protein catabolic process GO:0042176 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    proteasome complex GO:0000502 IDA
    proteasome complex GO:0000502 ISO
    nucleus GO:0005634 IBA
    proteasome regulatory particle GO:0005838 IDA
    proteasome regulatory particle, base subcomplex GO:0008540 IBA
    proteasome accessory complex GO:0022624 IDA
    proteasome storage granule GO:0034515 IBA
 Experiment description of studies that identified Psmd2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Psmd2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Farsa  
Co-fractionation Mus musculus
2 Psmb7 19177
Co-fractionation Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
5 Psme2 19188
Co-fractionation Mus musculus
6 Ubqln2  
Co-fractionation Mus musculus
7 Ap3d1  
Co-fractionation Mus musculus
8 Psmc3 19182
Co-fractionation Mus musculus
9 Psma2 19166
Co-fractionation Mus musculus
10 Psmb6 19175
Co-fractionation Mus musculus
11 Psmb4 19172
Co-fractionation Mus musculus
12 ABCG1  
Affinity Capture-MS Homo sapiens
13 Psmb5 19173
Co-fractionation Mus musculus
14 Psmc2 19181
Co-fractionation Mus musculus
15 Tcp1 21454
Co-fractionation Mus musculus
16 Psmd5 66998
Co-fractionation Mus musculus
17 Psmd4  
Co-fractionation Mus musculus
18 Psma3 19167
Co-fractionation Mus musculus
19 Psmd13 23997
Co-fractionation Mus musculus
20 Tssc1  
Co-fractionation Mus musculus
21 Psmd8 57296
Co-fractionation Mus musculus
22 Psmb1 19170
Co-fractionation Mus musculus
23 Atxn1  
Affinity Capture-MS Mus musculus
24 Ubqln1  
Co-fractionation Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 Txnl1 53382
Co-fractionation Mus musculus
27 Psmc6 67089
Co-fractionation Mus musculus
28 Vcp 269523
Co-fractionation Mus musculus
29 Psmd14 59029
Co-fractionation Mus musculus
30 Psmb8 16913
Co-fractionation Mus musculus
31 Psmc5 19184
Co-fractionation Mus musculus
32 Psmd9  
Co-fractionation Mus musculus
33 Cct2 12461
Co-fractionation Mus musculus
34 Psmc1 19179
Co-fractionation Mus musculus
35 Mettl21e  
Affinity Capture-MS Mus musculus
36 Psmd1 70247
Co-fractionation Mus musculus
37 Usp14 59025
Co-fractionation Mus musculus
38 Psmd7 17463
Co-fractionation Mus musculus
39 Ublcp1  
Co-fractionation Mus musculus
40 Zfand2a  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
41 Ubxn7  
Co-fractionation Mus musculus
42 Psmc4 23996
Co-fractionation Mus musculus
43 Nanog  
Affinity Capture-MS Mus musculus
44 Psmd3 22123
Co-fractionation Mus musculus
45 Psme1 19186
Co-fractionation Mus musculus
46 Uba5  
Co-fractionation Mus musculus
47 Etf1 225363
Co-fractionation Mus musculus
48 Itpr1  
Co-fractionation Mus musculus
49 Psmd6 66413
Co-fractionation Mus musculus
50 Psmd11 69077
Co-fractionation Mus musculus
51 Ubqln4  
Co-fractionation Mus musculus
52 Atg12  
Negative Genetic Mus musculus
53 Fancd2  
Affinity Capture-MS Mus musculus
54 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Psmd2 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport IEA Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Activation of NF-kappaB in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing-Cross presentation IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Assembly of the pre-replicative complex IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Autodegradation of the E3 ubiquitin ligase COP1 IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular response to hypoxia IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Cyclin A:Cdk2-associated events at S phase entry IEA Reactome
Cyclin E associated events during G1/S transition IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
Degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Deubiquitination IEA Reactome
DNA Replication IEA Reactome
DNA Replication Pre-Initiation IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream TCR signaling IEA Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G1/S DNA Damage Checkpoints IEA Reactome
G1/S Transition IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 IEA Reactome
Hedgehog 'off' state IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
KEAP1-NFE2L2 pathway IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of polyamines IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Neddylation IEA Reactome
Neutrophil degranulation IEA Reactome
NIK-->noncanonical NF-kB signaling IEA Reactome
Nuclear events mediated by NFE2L2 IEA Reactome
Orc1 removal from chromatin IEA Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha IEA Reactome
p53-Dependent G1 DNA Damage Response IEA Reactome
p53-Dependent G1/S DNA damage checkpoint IEA Reactome
p53-Independent DNA Damage Response IEA Reactome
p53-Independent G1/S DNA damage checkpoint IEA Reactome
PCP/CE pathway IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome
PTEN Regulation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of RAS by GAPs IEA Reactome
Regulation of RUNX2 expression and activity IEA Reactome
Regulation of RUNX3 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs IEA Reactome
S Phase IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by WNT IEA Reactome
Stabilization of p53 IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
TCR signaling IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
TNFR2 non-canonical NF-kB pathway IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transcriptional regulation by RUNX3 IEA Reactome
Transport of small molecules IEA Reactome
Ub-specific processing proteases IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D IEA Reactome
UCH proteinases IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here