Gene description for Usp14
Gene name ubiquitin specific peptidase 14
Gene symbol Usp14
Other names/aliases 2610005K12Rik
2610037B11Rik
AW107924
C78769
ax
nmf375
Species Mus musculus
 Database cross references - Usp14
ExoCarta ExoCarta_59025
Vesiclepedia VP_59025
Entrez Gene 59025
UniProt Q9JMA1  
 Usp14 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Usp14
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IEA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    endopeptidase inhibitor activity GO:0004866 IEA
    endopeptidase inhibitor activity GO:0004866 ISO
    protein binding GO:0005515 IPI
    K63-linked deubiquitinase activity GO:0061578 ISO
    proteasome binding GO:0070628 IBA
    proteasome binding GO:0070628 IEA
    proteasome binding GO:0070628 ISO
    deubiquitinase activity GO:0101005 ISO
Biological Process
    chemical synaptic transmission GO:0007268 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    innate immune response GO:0045087 IEA
    regulation of chemotaxis GO:0050920 IEA
    regulation of chemotaxis GO:0050920 ISO
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    protein K48-linked deubiquitination GO:0071108 IEA
    negative regulation of ERAD pathway GO:1904293 IBA
    negative regulation of ERAD pathway GO:1904293 IEA
    negative regulation of ERAD pathway GO:1904293 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 IEA
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
Subcellular Localization
    proteasome complex GO:0000502 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    cytoplasmic vesicle GO:0031410 IEA
    cytoplasmic vesicle GO:0031410 ISO
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 NAS
    presynaptic cytosol GO:0099523 IDA
    presynaptic cytosol GO:0099523 IEP
    presynaptic cytosol GO:0099523 IMP
    presynaptic cytosol GO:0099523 NAS
 Experiment description of studies that identified Usp14 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Usp14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmc3 19182
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Ube2v1 66589
Co-fractionation Mus musculus
4 Psmd2 21762
Co-fractionation Mus musculus
5 Psmd8 57296
Co-fractionation Mus musculus
6 Psma2 19166
Co-fractionation Mus musculus
7 Psmb6 19175
Co-fractionation Mus musculus
8 Gss  
Co-fractionation Mus musculus
9 Psmd12 66997
Co-fractionation Mus musculus
10 Psmd4  
Co-fractionation Mus musculus
11 Psma3 19167
Co-fractionation Mus musculus
12 Psmc2 19181
Co-fractionation Mus musculus
13 Uchl5 56207
Co-fractionation Mus musculus
14 Psmb1 19170
Co-fractionation Mus musculus
15 Ubxn2b  
Co-fractionation Mus musculus
16 Txnl1 53382
Co-fractionation Mus musculus
17 Rad23a  
Co-fractionation Mus musculus
18 Ufd1l  
Co-fractionation Mus musculus
19 Gabra1  
Two-hybrid Mus musculus
Co-localization Mus musculus
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
20 Psmd14 59029
Co-fractionation Mus musculus
21 Psmc5 19184
Co-fractionation Mus musculus
22 Echs1  
Co-fractionation Mus musculus
23 Psmc1 19179
Co-fractionation Mus musculus
24 Psmd7 17463
Co-fractionation Mus musculus
25 Psmf1  
Co-fractionation Mus musculus
26 Zfand2b  
Co-fractionation Mus musculus
27 Psmc4 23996
Co-fractionation Mus musculus
28 Unk  
Affinity Capture-RNA Mus musculus
29 Psmd3 22123
Co-fractionation Mus musculus
30 Psme1 19186
Co-fractionation Mus musculus
31 Adrm1  
Co-fractionation Mus musculus
32 Rpl30 19946
Co-fractionation Mus musculus
33 Eed  
Affinity Capture-MS Mus musculus
34 Psma4 26441
Co-fractionation Mus musculus
35 Psmd6 66413
Co-fractionation Mus musculus
36 Ubqln4  
Co-fractionation Mus musculus
37 Cndp2 66054
Co-fractionation Mus musculus
38 Ern1  
Affinity Capture-Western Mus musculus
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