Gene description for ACSF3
Gene name acyl-CoA synthetase family member 3
Gene symbol ACSF3
Other names/aliases -
Species Homo sapiens
 Database cross references - ACSF3
ExoCarta ExoCarta_197322
Vesiclepedia VP_197322
Entrez Gene 197322
HGNC 27288
MIM 614245
UniProt Q4G176  
 ACSF3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for ACSF3
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    acid-thiol ligase activity GO:0016878 IDA
    very long-chain fatty acid-CoA ligase activity GO:0031957 IBA
    very long-chain fatty acid-CoA ligase activity GO:0031957 TAS
    malonyl-CoA synthetase activity GO:0090409 IDA
Biological Process
    fatty acid metabolic process GO:0006631 IDA
    fatty acid biosynthetic process GO:0006633 IBA
    fatty acid biosynthetic process GO:0006633 IDA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 TAS
    malonate catabolic process GO:0090410 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ACSF3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ACSF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AK4 205
Affinity Capture-MS Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 MRPS7 51081
Affinity Capture-MS Homo sapiens
4 AMACR 23600
Affinity Capture-MS Homo sapiens
5 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 RAMP2  
Affinity Capture-MS Homo sapiens
8 MRPS2 51116
Affinity Capture-MS Homo sapiens
9 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
10 RAB28 9364
Two-hybrid Homo sapiens
11 MTPN 136319
Affinity Capture-MS Homo sapiens
12 ACSM5 54988
Affinity Capture-MS Homo sapiens
13 IMPDH1 3614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 OXLD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DNAJA2 10294
Affinity Capture-MS Homo sapiens
16 LONP1 9361
Affinity Capture-MS Homo sapiens
17 MATN4 8785
Two-hybrid Homo sapiens
18 KCTD14 65987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 HAUS2  
Affinity Capture-MS Homo sapiens
20 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
21 ACAA2 10449
Affinity Capture-MS Homo sapiens
22 PDHA1 5160
Proximity Label-MS Homo sapiens
23 TTBK1  
Affinity Capture-MS Homo sapiens
24 TRMT61B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 FAHD1 81889
Affinity Capture-MS Homo sapiens
27 PARK2  
Affinity Capture-MS Homo sapiens
28 TRIM43  
Affinity Capture-MS Homo sapiens
29 FAM174A 345757
Affinity Capture-MS Homo sapiens
30 METTL20  
Affinity Capture-MS Homo sapiens
31 SSBP1 6742
Proximity Label-MS Homo sapiens
32 AUP1 550
Affinity Capture-MS Homo sapiens
33 ABHD10 55347
Affinity Capture-MS Homo sapiens
34 PFDN5 5204
Affinity Capture-MS Homo sapiens
35 MOV10 4343
Affinity Capture-RNA Homo sapiens
36 RPA2 6118
Proximity Label-MS Homo sapiens
37 SDHB 6390
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TRIM27  
Two-hybrid Homo sapiens
39 FBXL18 80028
Affinity Capture-Western Homo sapiens
40 MYL10 93408
Affinity Capture-MS Homo sapiens
41 YARS2  
Affinity Capture-MS Homo sapiens
42 KRT40  
Two-hybrid Homo sapiens
43 MRPS24 64951
Affinity Capture-MS Homo sapiens
44 QRSL1  
Affinity Capture-MS Homo sapiens
45 TMEM184A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FTSJ2 29960
Affinity Capture-MS Homo sapiens
47 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RASL10B  
Affinity Capture-MS Homo sapiens
49 RPA3 6119
Proximity Label-MS Homo sapiens
50 MALSU1  
Affinity Capture-MS Homo sapiens
51 TMEM70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PDGFRA 5156
Affinity Capture-MS Homo sapiens
53 SLC25A10 1468
Affinity Capture-MS Homo sapiens
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