Gene description for Tsn
Gene name translin
Gene symbol Tsn
Other names/aliases 2610034C24Rik
AU040286
C3PO
TB-RBP
Species Mus musculus
 Database cross references - Tsn
ExoCarta ExoCarta_22099
Vesiclepedia VP_22099
Entrez Gene 22099
UniProt Q62348  
 Tsn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tsn
Molecular Function
    single-stranded DNA binding GO:0003697 IEA
    single-stranded DNA binding GO:0003697 ISO
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    mRNA binding GO:0003729 IDA
    endonuclease activity GO:0004519 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    sequence-specific DNA binding GO:0043565 IEA
    sequence-specific DNA binding GO:0043565 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    siRNA processing GO:0030422 ISO
    regulatory ncRNA-mediated post-transcriptional gene silencing GO:0035194 NAS
Subcellular Localization
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoribonuclease complex GO:1902555 ISO
 Experiment description of studies that identified Tsn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tsn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Wdr12  
Co-fractionation Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Tbcd  
Co-fractionation Mus musculus
4 Nsun2 28114
Co-fractionation Mus musculus
5 Ppp3ca 19055
Co-fractionation Mus musculus
6 Ube2i  
Two-hybrid Mus musculus
7 Tsnax  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
Co-fractionation Mus musculus
8 Nrd1 230598
Co-fractionation Mus musculus
9 TSNAX 7257
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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