Experiment description of studies that identified Nsun2 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 1117 MISEV standards ✘ Biophysical techniques ✔ Cd81|Icam1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F1R2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 1118 MISEV standards ✘ Biophysical techniques ✔ Cd81|Icam1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F1R2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Nsun2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Polr1a   Co-fractionation Mus musculus 2 D2Wsu81e   Co-fractionation Mus musculus 3 Ctps 51797 Co-fractionation Mus musculus 4 Cebpz   Co-fractionation Mus musculus 5 Trmt1   Co-fractionation Mus musculus 6 Arpc2 76709 Co-fractionation Mus musculus 7 Rbm28   Co-fractionation Mus musculus 8 Noc3l   Co-fractionation Mus musculus 9 Ube3a   Two-hybrid Mus musculus 10 Dis3   Co-fractionation Mus musculus 11 Thg1l   Co-fractionation Mus musculus 12 Pdcd11   Co-fractionation Mus musculus 13 Polr3b   Co-fractionation Mus musculus 14 Pwp2   Co-fractionation Mus musculus 15 Nop14   Co-fractionation Mus musculus 16 Rcl1   Co-fractionation Mus musculus 17 Ttll12 223723 Co-fractionation Mus musculus 18 Ddx27   Co-fractionation Mus musculus 19 Wdr6   Co-fractionation Mus musculus 20 Gar1   Co-fractionation Mus musculus 21 Uckl1   Co-fractionation Mus musculus 22 Ttc4   Co-fractionation Mus musculus 23 Imp3   Co-fractionation Mus musculus 24 Tsn 22099 Co-fractionation Mus musculus 25 Calu   Co-fractionation Mus musculus 26 Gnl2   Co-fractionation Mus musculus 27 Dcaf13 223499 Co-fractionation Mus musculus 28 Uggt1 320011 Co-fractionation Mus musculus 29 Pinx1   Co-fractionation Mus musculus 30 Gnl3   Co-fractionation Mus musculus 31 Rangap1   Co-fractionation Mus musculus 32 Utp11l   Co-fractionation Mus musculus 33 Ranbp6   Co-fractionation Mus musculus 34 Wdr43   Co-fractionation Mus musculus 35 Abcf2 27407 Co-fractionation Mus musculus 36 Lsg1   Co-fractionation Mus musculus 37 Gtpbp4   Co-fractionation Mus musculus 38 Nanog   Affinity Capture-MS Mus musculus 39 Sdad1   Co-fractionation Mus musculus 40 Dnajc21   Co-fractionation Mus musculus 41 Fancd2   Affinity Capture-MS Mus musculus 42 Lmo2   Two-hybrid Mus musculus Affinity Capture-Western Mus musculus 43 Eed   Affinity Capture-MS Mus musculus 44 Surf6   Co-fractionation Mus musculus 45 Hgh1 59053 Co-fractionation Mus musculus 46 Impdh1   Co-fractionation Mus musculus 47 Abce1 24015 Co-fractionation Mus musculus 48 Aatf   Co-fractionation Mus musculus View the network

Pathways in which Nsun2 is involved

No pathways found