Gene description for Ttll12
Gene name tubulin tyrosine ligase-like family, member 12
Gene symbol Ttll12
Other names/aliases BC055368
D430005B17
Species Mus musculus
 Database cross references - Ttll12
ExoCarta ExoCarta_223723
Vesiclepedia VP_223723
Entrez Gene 223723
UniProt Q3UDE2  
 Ttll12 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ttll12
Molecular Function
    ATP binding GO:0005524 IEA
    tubulin binding GO:0015631 IEA
    tubulin binding GO:0015631 ISO
    acid-amino acid ligase activity GO:0016881 IEA
    catalytic activity, acting on a protein GO:0140096 IEA
    H4K20me3 modified histone binding GO:1990889 IEA
    H4K20me3 modified histone binding GO:1990889 ISO
Biological Process
    regulation of mitotic cell cycle GO:0007346 IEA
    regulation of mitotic cell cycle GO:0007346 ISO
    protein modification process GO:0036211 IEA
    innate immune response GO:0045087 IEA
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IEA
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    spindle GO:0005819 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    midbody GO:0030496 IEA
 Experiment description of studies that identified Ttll12 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ttll12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPS21  
Affinity Capture-MS Homo sapiens
2 ZMYM1 79830
Affinity Capture-MS Homo sapiens
3 PGAM5 192111
Affinity Capture-MS Homo sapiens
4 Rprd1b  
Co-fractionation Mus musculus
5 TYRO3 7301
Affinity Capture-MS Homo sapiens
6 Nsun2 28114
Co-fractionation Mus musculus
7 HSPB8 26353
Affinity Capture-MS Homo sapiens
8 Eed  
Affinity Capture-MS Mus musculus
9 TPM1 7168
Affinity Capture-MS Homo sapiens
10 CBX3 11335
Affinity Capture-MS Homo sapiens
11 DNAJB1 3337
Affinity Capture-MS Homo sapiens
12 MRPL50 54534
Affinity Capture-MS Homo sapiens
13 EIF4B 1975
Affinity Capture-MS Homo sapiens
14 PSRC1  
Affinity Capture-MS Homo sapiens
15 DNAJB4 11080
Affinity Capture-MS Homo sapiens
16 BBS9 27241
Affinity Capture-MS Homo sapiens
17 OTUD6B 51633
Affinity Capture-MS Homo sapiens
18 KLHL7  
Affinity Capture-MS Homo sapiens
19 TTLL12 23170
Affinity Capture-MS Homo sapiens
20 Vbp1  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here