Gene description for Uba1
Gene name ubiquitin-like modifier activating enzyme 1
Gene symbol Uba1
Other names/aliases A1S9
Sbx
Ube-1
Ube1x
Species Mus musculus
 Database cross references - Uba1
ExoCarta ExoCarta_22201
Vesiclepedia VP_22201
Entrez Gene 22201
UniProt Q02053  
 Uba1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uba1
Molecular Function
    ubiquitin activating enzyme activity GO:0004839 IBA
    ubiquitin activating enzyme activity GO:0004839 IDA
    ubiquitin activating enzyme activity GO:0004839 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    DNA damage response GO:0006974 IBA
    DNA damage response GO:0006974 IEA
    DNA damage response GO:0006974 ISO
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    heterochromatin GO:0000792 IEA
    heterochromatin GO:0000792 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    endosome membrane GO:0010008 IEA
    endosome membrane GO:0010008 ISO
    desmosome GO:0030057 IEA
    desmosome GO:0030057 ISO
    rough endoplasmic reticulum membrane GO:0030867 IEA
    rough endoplasmic reticulum membrane GO:0030867 ISO
 Experiment description of studies that identified Uba1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uba1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf7  
Co-fractionation Mus musculus
2 PCM1 5108
Two-hybrid Homo sapiens
3 Ubc  
Reconstituted Complex Mus musculus
4 Cdk1 12534
Biochemical Activity Mus musculus
5 Pde4dip  
Affinity Capture-MS Mus musculus
6 Zfand5  
Co-fractionation Mus musculus
7 Stam2  
Co-fractionation Mus musculus
8 Ube2e3  
Co-fractionation Mus musculus
9 PIP 5304
Affinity Capture-MS Homo sapiens
10 Myl2  
Cross-Linking-MS (XL-MS) Mus musculus
11 Rnf114  
Co-fractionation Mus musculus
12 Uchl3 50933
Co-fractionation Mus musculus
13 Atxn1  
Affinity Capture-MS Mus musculus
14 UBA1 7317
Affinity Capture-MS Homo sapiens
15 Kcna3  
Affinity Capture-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
17 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
19 Tfe3  
Affinity Capture-MS Mus musculus
20 MTNR1A  
Reconstituted Complex Homo sapiens
21 Psmd14 59029
Co-fractionation Mus musculus
22 Ube2n 93765
Co-fractionation Mus musculus
23 Uchl1 22223
Co-fractionation Mus musculus
24 Ube2h  
Co-fractionation Mus musculus
25 Mettl21e  
Affinity Capture-MS Mus musculus
26 Impact  
Co-fractionation Mus musculus
27 Uba6 231380
Co-fractionation Mus musculus
28 Ube2k 53323
Co-fractionation Mus musculus
29 Tom1l2  
Co-fractionation Mus musculus
30 Ubxn6  
Co-fractionation Mus musculus
31 Unk  
Affinity Capture-RNA Mus musculus
32 Ube2r2  
Co-fractionation Mus musculus
33 SYCP1  
Affinity Capture-MS Homo sapiens
34 HSPH1 10808
Two-hybrid Homo sapiens
35 Eed  
Affinity Capture-MS Mus musculus
36 Tmcc2  
Co-fractionation Mus musculus
37 Mapt  
Affinity Capture-MS Mus musculus
38 Atg16l1  
Affinity Capture-MS Mus musculus
39 Ube2a  
Co-fractionation Mus musculus
40 Fancd2  
Affinity Capture-MS Mus musculus
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