Gene description for Ube2k
Gene name ubiquitin-conjugating enzyme E2K
Gene symbol Ube2k
Other names/aliases AW492011
D5Ertd601e
E2-25k
Hip2
Hypg
Lig
Species Mus musculus
 Database cross references - Ube2k
ExoCarta ExoCarta_53323
Vesiclepedia VP_53323
Entrez Gene 53323
UniProt P61087  
 Ube2k identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ube2k
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin protein ligase binding GO:0031625 ISS
    ubiquitin-ubiquitin ligase activity GO:0034450 IEA
    ubiquitin-ubiquitin ligase activity GO:0034450 ISO
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 ISO
    ubiquitin conjugating enzyme activity GO:0061631 TAS
Biological Process
    protein polyubiquitination GO:0000209 IBA
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 ISO
    free ubiquitin chain polymerization GO:0010994 IEA
    free ubiquitin chain polymerization GO:0010994 ISO
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IDA
    cellular response to interferon-beta GO:0035458 IEA
    cellular response to interferon-beta GO:0035458 ISO
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 IEA
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 ISO
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    protein K48-linked ubiquitination GO:0070936 ISS
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 IEA
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    filopodium tip GO:0032433 IDA
 Experiment description of studies that identified Ube2k in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ube2k
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
Biochemical Activity Mus musculus
3 RNF126  
Reconstituted Complex Homo sapiens
4 RNF151  
Reconstituted Complex Homo sapiens
5 UBL4A 8266
Affinity Capture-MS Homo sapiens
6 QARS 5859
Affinity Capture-MS Homo sapiens
7 NSMCE1  
Reconstituted Complex Homo sapiens
8 Trim2 80890
Co-fractionation Mus musculus
9 KLF16  
Affinity Capture-MS Homo sapiens
10 SDCCAG8  
Affinity Capture-MS Homo sapiens
11 UBA1 7317
Affinity Capture-MS Homo sapiens
12 NCOA2 10499
Affinity Capture-MS Homo sapiens
13 PUF60 22827
Affinity Capture-MS Homo sapiens
14 PPP1R10  
Affinity Capture-MS Homo sapiens
15 PHF20  
Affinity Capture-MS Homo sapiens
16 Rnf114  
Co-fractionation Mus musculus
17 RNF213 57674
Affinity Capture-MS Homo sapiens
18 PDS5A 23244
Affinity Capture-MS Homo sapiens
19 CYHR1  
Affinity Capture-MS Homo sapiens
20 Ube2d3  
Co-fractionation Mus musculus
21 Uba1 22201
Co-fractionation Mus musculus
22 ANXA7 310
Affinity Capture-MS Homo sapiens
23 Trim3  
Co-fractionation Mus musculus
View the network image/svg+xml



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