Gene description for SMG1
Gene name SMG1 phosphatidylinositol 3-kinase-related kinase
Gene symbol SMG1
Other names/aliases 61E3.4
ATX
LIP
Species Homo sapiens
 Database cross references - SMG1
ExoCarta ExoCarta_23049
Vesiclepedia VP_23049
Entrez Gene 23049
HGNC 30045
MIM 607032
UniProt Q96Q15  
 SMG1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMG1
Molecular Function
    RNA binding GO:0003723 HDA
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 NAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 EXP
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    telomeric DNA binding GO:0042162 IDA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IBA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IDA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 NAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    DNA repair GO:0006281 IEA
    chromatin remodeling GO:0006338 IEA
    mRNA export from nucleus GO:0006406 TAS
    DNA damage response GO:0006974 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 TAS
    regulation of telomere maintenance GO:0032204 IMP
    protein autophosphorylation GO:0046777 IDA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    chromatoid body GO:0033391 ISS
 Experiment description of studies that identified SMG1 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNBP 7555
Affinity Capture-MS Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 TTI1 9675
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 SIRT6  
Affinity Capture-MS Homo sapiens
5 NKX2-5  
Affinity Capture-MS Homo sapiens
6 RBM8A 9939
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 SMG8 55181
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
8 RIT1 6016
Negative Genetic Homo sapiens
9 KRT8 3856
Proximity Label-MS Homo sapiens
10 NPTN 27020
Affinity Capture-MS Homo sapiens
11 HAUS2  
Affinity Capture-MS Homo sapiens
12 SMG7  
Affinity Capture-Western Homo sapiens
13 NR3C1 2908
Proximity Label-MS Homo sapiens
14 EGFR 1956
Affinity Capture-MS Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 EIF4A3 9775
Co-fractionation Homo sapiens
17 UPF3A  
Affinity Capture-Western Homo sapiens
18 MKI67  
Affinity Capture-MS Homo sapiens
19 HTR6  
Affinity Capture-MS Homo sapiens
20 PRKCI 5584
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 RPA3 6119
Proximity Label-MS Homo sapiens
22 NECAP2 55707
Affinity Capture-MS Homo sapiens
23 SMG9 56006
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
24 C3orf18  
Affinity Capture-MS Homo sapiens
25 BTF3 689
Affinity Capture-MS Homo sapiens
26 UPF1 5976
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
27 RPS7 6201
Cross-Linking-MS (XL-MS) Homo sapiens
28 SMG1 23049
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
29 TELO2 9894
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 UPF3B 65109
Affinity Capture-Western Homo sapiens
31 KRAS 3845
Negative Genetic Homo sapiens
32 SIK2  
Affinity Capture-MS Homo sapiens
33 GSPT1 2935
Co-fractionation Homo sapiens
34 UPF2 26019
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 HSP90B1 7184
Affinity Capture-MS Homo sapiens
36 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TRIM71  
Affinity Capture-Western Homo sapiens
38 SMUG1  
Negative Genetic Homo sapiens
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