Gene description for TTC28
Gene name tetratricopeptide repeat domain 28
Gene symbol TTC28
Other names/aliases TPRBK
Species Homo sapiens
 Database cross references - TTC28
ExoCarta ExoCarta_23331
Vesiclepedia VP_23331
Entrez Gene 23331
HGNC 29179
MIM 615098
UniProt Q96AY4  
 TTC28 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TTC28
Molecular Function
    kinase binding GO:0019900 IPI
Biological Process
    regulation of mitotic cell cycle GO:0007346 IMP
    cell division GO:0051301 IEA
Subcellular Localization
    spindle pole GO:0000922 IEA
    cytoplasm GO:0005737 IEA
    centrosome GO:0005813 IEA
    midbody GO:0030496 IDA
 Experiment description of studies that identified TTC28 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TTC28
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAB 7529
Affinity Capture-MS Homo sapiens
2 YWHAH 7533
Affinity Capture-MS Homo sapiens
3 MAGEA8  
Affinity Capture-MS Homo sapiens
4 CD3D 915
Affinity Capture-MS Homo sapiens
5 MCOLN2  
Affinity Capture-MS Homo sapiens
6 YWHAG 7532
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PCDHGB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RNF7  
Affinity Capture-MS Homo sapiens
10 RAMP2  
Affinity Capture-MS Homo sapiens
11 JTB 10899
Affinity Capture-MS Homo sapiens
12 NCK1 4690
Affinity Capture-MS Homo sapiens
13 VWA8 23078
Proximity Label-MS Homo sapiens
14 IFNLR1  
Affinity Capture-MS Homo sapiens
15 ATG5 9474
Proximity Label-MS Homo sapiens
16 TP53 7157
Synthetic Growth Defect Homo sapiens
17 ICAM1 3383
Affinity Capture-MS Homo sapiens
18 SSR2  
Affinity Capture-MS Homo sapiens
19 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NINL  
Proximity Label-MS Homo sapiens
21 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
22 LRRC25  
Affinity Capture-MS Homo sapiens
23 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 YWHAE 7531
Affinity Capture-MS Homo sapiens
25 P4HA2 8974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RNF43  
Proximity Label-MS Homo sapiens
27 SEMA4C 54910
Affinity Capture-MS Homo sapiens
28 P4HA1 5033
Affinity Capture-MS Homo sapiens
29 SSPO 23145
Two-hybrid Homo sapiens
30 HRAS 3265
Reconstituted Complex Homo sapiens
31 TSPYL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SQSTM1 8878
Proximity Label-MS Homo sapiens
33 SNCAIP  
Affinity Capture-MS Homo sapiens
34 UBC 7316
Reconstituted Complex Homo sapiens
35 MAMDC4  
Affinity Capture-MS Homo sapiens
36 HCST  
Affinity Capture-MS Homo sapiens
37 CD79A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 BRE 9577
Affinity Capture-MS Homo sapiens
39 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RPA4  
Proximity Label-MS Homo sapiens
41 MAPRE3  
Proximity Label-MS Homo sapiens
42 NCKAP1 10787
Affinity Capture-MS Homo sapiens
43 PXN 5829
Proximity Label-MS Homo sapiens
44 NIN 51199
Proximity Label-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 BICD1 636
Proximity Label-MS Homo sapiens
47 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GOLGA1  
Proximity Label-MS Homo sapiens
49 ORAI1 84876
Affinity Capture-MS Homo sapiens
50 HSPA1A 3303
Affinity Capture-MS Homo sapiens
51 LAMP3  
Affinity Capture-MS Homo sapiens
52 IMPDH2 3615
Proximity Label-MS Homo sapiens
53 PRMT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 S100B 6285
Affinity Capture-MS Homo sapiens
55 BRPF3  
Affinity Capture-MS Homo sapiens
56 GRIN2A  
Affinity Capture-MS Homo sapiens
57 KHDC1  
Affinity Capture-MS Homo sapiens
58 TRMT2A 27037
Affinity Capture-MS Homo sapiens
59 NR3C1 2908
Proximity Label-MS Homo sapiens
60 SDF4 51150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MYCBPAP 84073
Affinity Capture-MS Homo sapiens
62 NPTN 27020
Affinity Capture-MS Homo sapiens
63 AGPS 8540
Proximity Label-MS Homo sapiens
64 HTRA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CPE 1363
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TTC28 is involved
No pathways found





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