Gene description for MTHFD1L
Gene name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Gene symbol MTHFD1L
Other names/aliases FTHFSDC1
MTC1THFS
dJ292B18.2
Species Homo sapiens
 Database cross references - MTHFD1L
ExoCarta ExoCarta_25902
Vesiclepedia VP_25902
Entrez Gene 25902
HGNC 21055
MIM 611427
UniProt Q6UB35  
 MTHFD1L identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MTHFD1L
Molecular Function
    formate-tetrahydrofolate ligase activity GO:0004329 IBA
    formate-tetrahydrofolate ligase activity GO:0004329 IDA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IEA
    ATP binding GO:0005524 IDA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    neural tube closure GO:0001843 ISS
    folic acid-containing compound metabolic process GO:0006760 IDA
    10-formyltetrahydrofolate biosynthetic process GO:0009257 IBA
    10-formyltetrahydrofolate biosynthetic process GO:0009257 IDA
    formate metabolic process GO:0015942 IDA
    tetrahydrofolate interconversion GO:0035999 IEA
    embryonic neurocranium morphogenesis GO:0048702 ISS
    embryonic viscerocranium morphogenesis GO:0048703 ISS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified MTHFD1L in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MTHFD1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C9orf78 51759
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 CASP4  
Affinity Capture-MS Homo sapiens
4 PDHA1 5160
Proximity Label-MS Homo sapiens
5 ANLN 54443
Affinity Capture-MS Homo sapiens
6 CLPP 8192
Proximity Label-MS Homo sapiens
7 DNAJC28  
Proximity Label-MS Homo sapiens
8 CS 1431
Proximity Label-MS Homo sapiens
9 BPHL 670
Co-fractionation Homo sapiens
10 CNN3 1266
Co-fractionation Homo sapiens
11 HOOK1  
Proximity Label-MS Homo sapiens
12 TSFM 10102
Proximity Label-MS Homo sapiens
13 USP50  
Affinity Capture-MS Homo sapiens
14 MTIF2 4528
Proximity Label-MS Homo sapiens
15 MRRF  
Proximity Label-MS Homo sapiens
16 AIFM1 9131
Proximity Label-MS Homo sapiens
17 RPS24 6229
Cross-Linking-MS (XL-MS) Homo sapiens
18 KIF14 9928
Affinity Capture-MS Homo sapiens
19 ATG5 9474
Proximity Label-MS Homo sapiens
20 AKAP1 8165
Affinity Capture-MS Homo sapiens
21 CNN1 1264
Co-fractionation Homo sapiens
22 MARC2 54996
Affinity Capture-MS Homo sapiens
23 MDH2 4191
Proximity Label-MS Homo sapiens
24 PRMT1 3276
Affinity Capture-MS Homo sapiens
25 SIRT7  
Affinity Capture-MS Homo sapiens
26 APOO 79135
Proximity Label-MS Homo sapiens
27 RPA2 6118
Proximity Label-MS Homo sapiens
28 NFATC1 4772
Affinity Capture-MS Homo sapiens
29 ADRB2  
Affinity Capture-MS Homo sapiens
30 CMPK1 51727
Co-fractionation Homo sapiens
31 KIAA1429 25962
Affinity Capture-MS Homo sapiens
32 C6orf203  
Proximity Label-MS Homo sapiens
33 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ACSM5 54988
Affinity Capture-MS Homo sapiens
35 PAPOLB  
Co-fractionation Homo sapiens
36 NDUFS7 374291
Affinity Capture-MS Homo sapiens
37 FAHD1 81889
Affinity Capture-MS Homo sapiens
38 COX8A  
Proximity Label-MS Homo sapiens
39 HOOK3 84376
Proximity Label-MS Homo sapiens
40 PARK2  
Affinity Capture-MS Homo sapiens
41 Ncaph  
Affinity Capture-MS Mus musculus
42 C21orf33  
Proximity Label-MS Homo sapiens
43 RIPK4  
Affinity Capture-MS Homo sapiens
44 AMACR 23600
Affinity Capture-MS Homo sapiens
45 WRAP73 49856
Affinity Capture-MS Homo sapiens
46 LOC100132735  
Protein-RNA Homo sapiens
47 LRPPRC 10128
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
48 ASB12  
Affinity Capture-MS Homo sapiens
49 CAPZB 832
Affinity Capture-MS Homo sapiens
50 RPA3 6119
Proximity Label-MS Homo sapiens
51 HSCB 150274
Proximity Label-MS Homo sapiens
52 PAPOLA 10914
Co-fractionation Homo sapiens
53 MAGED1 9500
Affinity Capture-MS Homo sapiens
54 TBRG4 9238
Proximity Label-MS Homo sapiens
55 TAGLN2 8407
Co-fractionation Homo sapiens
56 AARS2  
Proximity Label-MS Homo sapiens
57 MTMR7  
Affinity Capture-MS Homo sapiens
58 MYH9 4627
Affinity Capture-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 SPRTN  
Affinity Capture-MS Homo sapiens
61 COPS5 10987
Affinity Capture-MS Homo sapiens
62 C1QBP 708
Proximity Label-MS Homo sapiens
63 YARS2  
Affinity Capture-MS Homo sapiens
64 HINT2 84681
Proximity Label-MS Homo sapiens
65 HGS 9146
Reconstituted Complex Homo sapiens
66 SNX27 81609
Affinity Capture-MS Homo sapiens
67 NPM1 4869
Affinity Capture-MS Homo sapiens
68 UFL1 23376
Affinity Capture-MS Homo sapiens
69 ISOC1 51015
Co-fractionation Homo sapiens
70 QRSL1  
Affinity Capture-MS Homo sapiens
71 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
72 METTL17  
Proximity Label-MS Homo sapiens
73 MARS2 92935
Affinity Capture-MS Homo sapiens
74 ANGEL1  
Affinity Capture-MS Homo sapiens
75 HECW2  
Affinity Capture-MS Homo sapiens
76 MALSU1  
Affinity Capture-MS Homo sapiens
77 MKI67  
Affinity Capture-MS Homo sapiens
78 NDUFS6  
Affinity Capture-MS Homo sapiens
79 C9orf72  
Affinity Capture-MS Homo sapiens
80 CYLD  
Affinity Capture-MS Homo sapiens
81 EXD2  
Proximity Label-MS Homo sapiens
82 XPNPEP1 7511
Co-fractionation Homo sapiens
83 RPS16 6217
Affinity Capture-MS Homo sapiens
84 TRAPPC6B 122553
Affinity Capture-MS Homo sapiens
85 COX4I1 1327
Proximity Label-MS Homo sapiens
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