Gene description for PAPOLA
Gene name poly(A) polymerase alpha
Gene symbol PAPOLA
Other names/aliases PAP
Species Homo sapiens
 Database cross references - PAPOLA
ExoCarta ExoCarta_10914
Vesiclepedia VP_10914
Entrez Gene 10914
HGNC 14981
MIM 605553
UniProt P51003  
 PAPOLA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PAPOLA
Molecular Function
    magnesium ion binding GO:0000287 ISS
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
    manganese ion binding GO:0030145 ISS
    poly(A) RNA polymerase activity GO:1990817 IBA
    poly(A) RNA polymerase activity GO:1990817 IDA
    poly(A) RNA polymerase activity GO:1990817 ISS
    poly(A) RNA polymerase activity GO:1990817 TAS
Biological Process
    mRNA 3'-end processing GO:0031124 IDA
    mRNA 3'-end processing GO:0031124 TAS
    co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway GO:0180010 IDA
    cytosolic mRNA polyadenylation GO:0180011 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified PAPOLA in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PAPOLA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BACE2 25825
Affinity Capture-MS Homo sapiens
2 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 CPSF6 11052
Affinity Capture-Western Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 MRPL42  
Affinity Capture-MS Homo sapiens
7 UFD1L 7353
Co-fractionation Homo sapiens
8 ADRBK1 156
Co-fractionation Homo sapiens
9 PPP1CB 5500
Co-fractionation Homo sapiens
10 CPSF1 29894
Reconstituted Complex Homo sapiens
11 FAM129B 64855
Co-fractionation Homo sapiens
12 SARS 6301
Co-fractionation Homo sapiens
13 YAF2  
Affinity Capture-MS Homo sapiens
14 UGP2 7360
Co-fractionation Homo sapiens
15 TMPO 7112
Co-fractionation Homo sapiens
16 TP53 7157
Affinity Capture-MS Homo sapiens
17 TGIF2  
Affinity Capture-MS Homo sapiens
18 SIRT2 22933
Affinity Capture-Western Homo sapiens
19 BAG2 9532
Affinity Capture-MS Homo sapiens
20 CIRBP 1153
Affinity Capture-MS Homo sapiens
21 ZYX 7791
Co-fractionation Homo sapiens
22 REL 5966
Affinity Capture-MS Homo sapiens
23 AURKB 9212
Affinity Capture-MS Homo sapiens
24 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
25 ACTC1 70
Proximity Label-MS Homo sapiens
26 WDR33 55339
Co-fractionation Homo sapiens
27 PROSC 11212
Co-fractionation Homo sapiens
28 FGB 2244
Affinity Capture-MS Homo sapiens
29 CSTF2 1478
Co-fractionation Homo sapiens
30 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
31 CSTF3 1479
Co-fractionation Homo sapiens
32 CREBBP  
Affinity Capture-Western Homo sapiens
33 HNRNPA1 3178
Co-fractionation Homo sapiens
34 PUF60 22827
Co-fractionation Homo sapiens
35 MTHFD1 4522
Co-fractionation Homo sapiens
36 SUPT5H 6829
Co-fractionation Homo sapiens
37 DDX58 23586
Affinity Capture-RNA Homo sapiens
38 TARS 6897
Co-fractionation Homo sapiens
39 RSRC1  
Affinity Capture-MS Homo sapiens
40 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
41 HDAC1 3065
Affinity Capture-Western Homo sapiens
42 APEX1 328
Affinity Capture-RNA Homo sapiens
43 HDAC10  
Affinity Capture-Western Homo sapiens
44 CPSF2 53981
Co-fractionation Homo sapiens
45 PLOD2 5352
Co-fractionation Homo sapiens
46 HDAC3 8841
Affinity Capture-Western Homo sapiens
47 PABPC1 26986
Co-fractionation Homo sapiens
48 CORO1B 57175
Co-fractionation Homo sapiens
49 YAP1 10413
Co-fractionation Homo sapiens
50 CSTF2T  
Co-fractionation Homo sapiens
51 HSPA2 3306
Affinity Capture-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 BAP1 8314
Negative Genetic Homo sapiens
54 NRAS 4893
Affinity Capture-MS Homo sapiens
55 SMAD2 4087
Two-hybrid Homo sapiens
56 NUDT21 11051
Affinity Capture-Western Homo sapiens
57 KPNB1 3837
Affinity Capture-Western Homo sapiens
58 CCDC53 51019
Affinity Capture-MS Homo sapiens
59 SERBP1 26135
Affinity Capture-MS Homo sapiens
60 NARS 4677
Co-fractionation Homo sapiens
61 SIRT1  
Affinity Capture-Western Homo sapiens
62 MEN1 4221
Affinity Capture-MS Homo sapiens
63 PABPC3 5042
Co-fractionation Homo sapiens
64 NUDC 10726
Co-fractionation Homo sapiens
65 PICALM 8301
Co-fractionation Homo sapiens
66 CORO1C 23603
Co-fractionation Homo sapiens
67 GNA11 2767
Affinity Capture-MS Homo sapiens
68 HUWE1 10075
Affinity Capture-MS Homo sapiens
69 MTHFD1L 25902
Co-fractionation Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
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