Gene description for GPSM1
Gene name G-protein signaling modulator 1
Gene symbol GPSM1
Other names/aliases AGS3
Species Homo sapiens
 Database cross references - GPSM1
ExoCarta ExoCarta_26086
Vesiclepedia VP_26086
Entrez Gene 26086
HGNC 17858
MIM 609491
UniProt Q86YR5  
 GPSM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GPSM1
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IBA
    GDP-dissociation inhibitor activity GO:0005092 IBA
    GDP-dissociation inhibitor activity GO:0005092 IDA
    protein binding GO:0005515 IPI
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    nervous system development GO:0007399 IEA
    positive regulation of macroautophagy GO:0016239 IDA
    cell differentiation GO:0030154 IEA
    negative regulation of GTPase activity GO:0034260 IDA
    negative regulation of guanyl-nucleotide exchange factor activity GO:1905098 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IDA
    cell cortex GO:0005938 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified GPSM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GPSM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIP13 9319
Two-hybrid Homo sapiens
2 KLHL18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NUMA1 4926
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PPP6R3 55291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 SEPT1 1731
Two-hybrid Homo sapiens
7 NPAS1  
Affinity Capture-MS Homo sapiens
8 UGP2 7360
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 XPO1 7514
Affinity Capture-MS Homo sapiens
10 FAM208B 54906
Two-hybrid Homo sapiens
11 DACH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 OSTM1 28962
Reconstituted Complex Homo sapiens
13 TEKT4  
Two-hybrid Homo sapiens
14 GPSM1 26086
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 FAM161B  
Two-hybrid Homo sapiens
16 VEZF1  
Two-hybrid Homo sapiens
17 CUL3 8452
Affinity Capture-MS Homo sapiens
18 USP9X 8239
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 CCDC158 339965
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 BHLHE40  
Two-hybrid Homo sapiens
21 DCAF11  
Two-hybrid Homo sapiens
22 PARK2  
Affinity Capture-MS Homo sapiens
23 FAM90A1  
Two-hybrid Homo sapiens
24 GLIS2  
Two-hybrid Homo sapiens
25 GNAI1 2770
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SAPCD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CHAMP1  
Affinity Capture-MS Homo sapiens
28 DAZAP2  
Two-hybrid Homo sapiens
29 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
30 POGZ 23126
Affinity Capture-MS Homo sapiens
31 NTRK1 4914
Affinity Capture-MS Homo sapiens
32 FAM83A  
Two-hybrid Homo sapiens
33 RIBC1  
Two-hybrid Homo sapiens
34 KANK2 25959
Two-hybrid Homo sapiens
35 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 USHBP1  
Two-hybrid Homo sapiens
37 GCM2  
Two-hybrid Homo sapiens
38 INSC  
Affinity Capture-MS Homo sapiens
39 BRD4 23476
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HMG20A  
Two-hybrid Homo sapiens
41 METTL21B  
Affinity Capture-MS Homo sapiens
42 GNAI3 2773
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
43 ZBED1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GPSM1 is involved
PathwayEvidenceSource
G alpha (i) signalling events TAS Reactome
GPCR downstream signalling TAS Reactome
Signal Transduction TAS Reactome
Signaling by GPCR TAS Reactome





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