Gene description for WSB1
Gene name WD repeat and SOCS box containing 1
Gene symbol WSB1
Other names/aliases SWIP1
WSB-1
Species Homo sapiens
 Database cross references - WSB1
ExoCarta ExoCarta_26118
Vesiclepedia VP_26118
Entrez Gene 26118
HGNC 19221
MIM 610091
UniProt Q9Y6I7  
 WSB1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for WSB1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IEA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    intracellular signal transduction GO:0035556 IEA
    protein K27-linked ubiquitination GO:0044314 IEA
    protein aggregate center assembly GO:0140454 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified WSB1 in sEVs
1
Experiment ID 236
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for WSB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BTRC 8945
Affinity Capture-Western Homo sapiens
2 ARIH2 10425
Affinity Capture-MS Homo sapiens
3 TCEB1 6921
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 GSK3B 2932
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 CSNK1A1 1452
Affinity Capture-Western Homo sapiens
6 SREBF1 6720
Negative Genetic Homo sapiens
7 CUL5 8065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 VHL  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
9 AXIN1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 RNF7  
Affinity Capture-MS Homo sapiens
11 ARHGDIB 397
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 UBE2D2 7322
Reconstituted Complex Homo sapiens
13 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
14 CSF3R  
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
15 LRRK2 120892
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
16 DDB1 1642
Affinity Capture-Western Homo sapiens
17 PPP2CA 5515
Affinity Capture-Western Homo sapiens
18 TCEB2 6923
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 HIPK2 28996
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
20 UBE2D3 7323
Reconstituted Complex Homo sapiens
21 DIO2  
FRET Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
22 CUL4A 8451
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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