Gene description for Map2k1
Gene name mitogen-activated protein kinase kinase 1
Gene symbol Map2k1
Other names/aliases MAPKK1
MEKK1
Mek1
Prkmk1
Species Mus musculus
 Database cross references - Map2k1
ExoCarta ExoCarta_26395
Vesiclepedia VP_26395
Entrez Gene 26395
UniProt P31938  
 Map2k1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Map2k1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    MAP kinase kinase activity GO:0004708 IBA
    MAP kinase kinase activity GO:0004708 IDA
    MAP kinase kinase activity GO:0004708 IMP
    MAP kinase kinase activity GO:0004708 ISO
    protein serine/threonine/tyrosine kinase activity GO:0004712 TAS
    MAP-kinase scaffold activity GO:0005078 IEA
    MAP-kinase scaffold activity GO:0005078 ISO
    protein binding GO:0005515 IPI
    protein binding GO:0005515 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    JUN kinase kinase activity GO:0008545 IEA
    protein kinase binding GO:0019901 ISO
    small GTPase binding GO:0031267 ISO
    mitogen-activated protein kinase kinase kinase binding GO:0031435 ISO
    histone H3Y41 kinase activity GO:0035401 IEA
    protein serine/threonine kinase activator activity GO:0043539 IEA
    protein serine/threonine kinase activator activity GO:0043539 ISO
    protein-containing complex binding GO:0044877 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    MAPK cascade GO:0000165 IGI
    MAPK cascade GO:0000165 IMP
    regulation of vascular associated smooth muscle contraction GO:0003056 ISO
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IGI
    protein phosphorylation GO:0006468 IMP
    response to oxidative stress GO:0006979 ISO
    JNK cascade GO:0007254 IEA
    heart development GO:0007507 IGI
    negative regulation of cell population proliferation GO:0008285 IEA
    negative regulation of cell population proliferation GO:0008285 ISO
    insulin receptor signaling pathway GO:0008286 ISO
    positive regulation of autophagy GO:0010508 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 ISO
    Schwann cell development GO:0014044 IMP
    cerebellar cortex formation GO:0021697 IGI
    neuron differentiation GO:0030182 IBA
    neuron differentiation GO:0030182 IMP
    neuron differentiation GO:0030182 ISO
    keratinocyte differentiation GO:0030216 IMP
    positive regulation of cell migration GO:0030335 ISO
    thyroid gland development GO:0030878 IGI
    melanosome transport GO:0032402 ISO
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 ISO
    negative regulation of homotypic cell-cell adhesion GO:0034111 ISO
    endodermal cell differentiation GO:0035987 IDA
    ERBB2-ERBB3 signaling pathway GO:0038133 IDA
    myelination GO:0042552 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    positive regulation of MAPK cascade GO:0043410 ISO
    type B pancreatic cell proliferation GO:0044342 IMP
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of muscle contraction GO:0045933 ISO
    positive regulation of Ras protein signal transduction GO:0046579 ISO
    vesicle transport along microtubule GO:0047496 ISO
    insulin-like growth factor receptor signaling pathway GO:0048009 IMP
    Golgi inheritance GO:0048313 ISO
    thymus development GO:0048538 IGI
    response to axon injury GO:0048678 ISO
    regulation of axon regeneration GO:0048679 IGI
    neuron projection morphogenesis GO:0048812 ISO
    cell motility GO:0048870 IMP
    positive regulation of axonogenesis GO:0050772 IGI
    response to glucocorticoid GO:0051384 ISO
    Bergmann glial cell differentiation GO:0060020 IGI
    face development GO:0060324 IGI
    lung morphogenesis GO:0060425 IGI
    trachea formation GO:0060440 IGI
    epithelial cell proliferation involved in lung morphogenesis GO:0060502 IGI
    placenta blood vessel development GO:0060674 IMP
    labyrinthine layer development GO:0060711 IMP
    triglyceride homeostasis GO:0070328 ISO
    ERK1 and ERK2 cascade GO:0070371 IGI
    regulation of ERK1 and ERK2 cascade GO:0070372 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    regulation of Golgi inheritance GO:0090170 TAS
    cellular senescence GO:0090398 IEA
    cellular senescence GO:0090398 ISO
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 ISO
    positive regulation of endodermal cell differentiation GO:1903226 IDA
    negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway GO:1903298 ISO
    regulation of early endosome to late endosome transport GO:2000641 TAS
    positive regulation of ATP biosynthetic process GO:2001171 ISO
Subcellular Localization
    nucleus GO:0005634 TAS
    mitochondrion GO:0005739 TAS
    early endosome GO:0005769 TAS
    late endosome GO:0005770 TAS
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 TAS
    centrosome GO:0005813 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    microtubule GO:0005874 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 TAS
    cell cortex GO:0005938 ISO
    postsynaptic density GO:0014069 ISO
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    dendrite cytoplasm GO:0032839 ISO
    perikaryon GO:0043204 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Map2k1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Map2k1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pebp1 23980
Affinity Capture-Western Mus musculus
2 Syngap1  
Affinity Capture-MS Mus musculus
3 Nek10 674895
Affinity Capture-Western Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Axin1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 Foxp3  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Lamtor2  
Co-fractionation Mus musculus
9 CPNE1 8904
Reconstituted Complex Homo sapiens
10 Raf1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
11 Tmem173  
Proximity Label-MS Mus musculus
12 Brap  
Affinity Capture-Western Mus musculus
13 Grin2b  
Affinity Capture-Western Mus musculus
14 Map3k1  
Affinity Capture-Western Mus musculus
15 Braf 109880
Two-hybrid Mus musculus
16 Agap2  
Affinity Capture-MS Mus musculus
17 Mapk3 26417
Co-fractionation Mus musculus
18 Grin1  
Affinity Capture-Western Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Mapk1 26413
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
21 Ywhae 22627
Co-fractionation Mus musculus
22 Mapt  
Affinity Capture-MS Mus musculus
23 Tia1  
Affinity Capture-MS Mus musculus
24 Ksr1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
25 Cflar  
Affinity Capture-Western Mus musculus
26 Pxn  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
27 CPNE4 131034
Reconstituted Complex Homo sapiens
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