Gene description for TSSK4
Gene name testis-specific serine kinase 4
Gene symbol TSSK4
Other names/aliases C14orf20
STK22E
TSK-4
TSK4
TSSK5
Species Homo sapiens
 Database cross references - TSSK4
ExoCarta ExoCarta_283629
Vesiclepedia VP_283629
Entrez Gene 283629
HGNC 19825
MIM 610711
UniProt Q6SA08  
 TSSK4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for TSSK4
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 ISS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 ISS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    protein homodimerization activity GO:0042803 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-containing complex binding GO:0044877 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISS
    spermatid development GO:0007286 IEA
    fertilization GO:0009566 IEA
    flagellated sperm motility GO:0030317 IEA
    positive regulation of CREB transcription factor activity GO:0032793 IDA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    sperm flagellum GO:0036126 IEA
 Experiment description of studies that identified TSSK4 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TSSK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACACB 32
Affinity Capture-MS Homo sapiens
2 CREB1  
Two-hybrid Homo sapiens
3 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
4 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
5 WDR3  
Affinity Capture-MS Homo sapiens
6 BRWD3  
Affinity Capture-MS Homo sapiens
7 TAF1  
Affinity Capture-MS Homo sapiens
8 H2AFX 3014
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TSSK4 is involved
No pathways found





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