Gene description for SLC9A9
Gene name solute carrier family 9, subfamily A (NHE9, cation proton antiporter 9), member 9
Gene symbol SLC9A9
Other names/aliases AUTS16
NHE9
Species Homo sapiens
 Database cross references - SLC9A9
ExoCarta ExoCarta_285195
Vesiclepedia VP_285195
Entrez Gene 285195
HGNC 20653
MIM 608396
UniProt Q8IVB4  
 SLC9A9 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SLC9A9
Molecular Function
    protein binding GO:0005515 IPI
    sodium:proton antiporter activity GO:0015385 IBA
    sodium:proton antiporter activity GO:0015385 TAS
    potassium:proton antiporter activity GO:0015386 IBA
    potassium:proton antiporter activity GO:0015386 ISS
Biological Process
    monoatomic ion transport GO:0006811 TAS
    defense response to bacterium GO:0042742 IEA
    regulation of intracellular pH GO:0051453 IBA
    regulation of intracellular pH GO:0051453 IDA
    potassium ion transmembrane transport GO:0071805 IBA
    potassium ion transmembrane transport GO:0071805 ISS
    phagosome maturation GO:0090382 IEA
    sodium ion import across plasma membrane GO:0098719 IBA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    early endosome GO:0005769 IDA
    plasma membrane GO:0005886 IBA
    phagocytic vesicle membrane GO:0030670 IEA
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 TAS
    early phagosome GO:0032009 IEA
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IDA
 Experiment description of studies that identified SLC9A9 in sEVs
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC9A9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COX5A 9377
Affinity Capture-MS Homo sapiens
2 MAP2K2 5605
Affinity Capture-Western Homo sapiens
3 ARL1 400
Affinity Capture-MS Homo sapiens
4 NDUFA6  
Affinity Capture-MS Homo sapiens
5 STT3A 3703
Affinity Capture-MS Homo sapiens
6 TFRC 7037
Affinity Capture-Western Homo sapiens
7 PTMA 5757
Affinity Capture-MS Homo sapiens
8 VIM 7431
Affinity Capture-MS Homo sapiens
9 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 PDCD6 10016
Affinity Capture-MS Homo sapiens
11 PLOD1 5351
Affinity Capture-MS Homo sapiens
12 UQCR10 29796
Affinity Capture-MS Homo sapiens
13 ARF6 382
Affinity Capture-MS Homo sapiens
14 GNB1 2782
Affinity Capture-MS Homo sapiens
15 ATP5G1  
Affinity Capture-MS Homo sapiens
16 OPRM1 4988
Two-hybrid Homo sapiens
17 RAB5C 5878
Affinity Capture-MS Homo sapiens
18 SLC9A9 285195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 VDAC1 7416
Affinity Capture-MS Homo sapiens
20 HSPA9 3313
Affinity Capture-MS Homo sapiens
21 VDAC2 7417
Affinity Capture-MS Homo sapiens
22 FLOT1 10211
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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