Experiment description of studies that identified NANOS1 in sEVs

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for NANOS1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CNOT7 29883 Affinity Capture-MS Homo sapiens 2 TNKS1BP1 85456 Affinity Capture-MS Homo sapiens 3 CNOT6L 246175 Affinity Capture-MS Homo sapiens 4 CNOT1 23019 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Co-crystal Structure Homo sapiens Affinity Capture-MS Homo sapiens 5 PHYH 5264 Affinity Capture-MS Homo sapiens 6 CNOT2   Affinity Capture-MS Homo sapiens 7 CNOT10 25904 Affinity Capture-MS Homo sapiens 8 CNOT11 55571 Affinity Capture-MS Homo sapiens 9 PUM2 23369 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 10 CNOT6   Affinity Capture-MS Homo sapiens 11 FHL2 2274 Affinity Capture-MS Homo sapiens 12 KLHDC8B 200942 Affinity Capture-MS Homo sapiens 13 CNOT3 4849 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 14 CNOT8   Affinity Capture-MS Homo sapiens 15 PAK1 5058 Affinity Capture-MS Homo sapiens 16 C17orf70   Affinity Capture-MS Homo sapiens View the network

Pathways in which NANOS1 is involved

No pathways found