Gene description for PLXNA2
Gene name plexin A2
Gene symbol PLXNA2
Other names/aliases OCT
PLXN2
Species Homo sapiens
 Database cross references - PLXNA2
ExoCarta ExoCarta_5362
Vesiclepedia VP_5362
Entrez Gene 5362
HGNC 9100
MIM 601054
UniProt O75051  
 PLXNA2 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for PLXNA2
Molecular Function
    protein binding GO:0005515 IPI
    semaphorin receptor activity GO:0017154 IBA
    semaphorin receptor activity GO:0017154 ISS
    identical protein binding GO:0042802 IEA
Biological Process
    somitogenesis GO:0001756 IEA
    synapse assembly GO:0007416 IBA
    neural tube development GO:0021915 IEA
    cerebellar granule cell precursor tangential migration GO:0021935 IEA
    regulation of cell migration GO:0030334 IBA
    regulation of cell migration GO:0030334 ISS
    centrosome localization GO:0051642 IEA
    pharyngeal system development GO:0060037 IEA
    limb bud formation GO:0060174 IEA
    semaphorin-plexin signaling pathway GO:0071526 IBA
    semaphorin-plexin signaling pathway GO:0071526 ISS
Subcellular Localization
    semaphorin receptor complex GO:0002116 IBA
    semaphorin receptor complex GO:0002116 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified PLXNA2 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for PLXNA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRLF2 64109
Affinity Capture-MS Homo sapiens
2 RYK 6259
Affinity Capture-MS Homo sapiens
3 ADAM30 11085
Affinity Capture-MS Homo sapiens
4 GPM6A 2823
Affinity Capture-MS Homo sapiens
5 LYZL6  
Affinity Capture-MS Homo sapiens
6 GPIHBP1  
Affinity Capture-MS Homo sapiens
7 FNBP4  
Two-hybrid Homo sapiens
8 PSCA 8000
Affinity Capture-MS Homo sapiens
9 CCL22 6367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CA6 765
Affinity Capture-MS Homo sapiens
11 WFDC11  
Affinity Capture-MS Homo sapiens
12 RETNLB  
Affinity Capture-MS Homo sapiens
13 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MFAP4 4239
Affinity Capture-MS Homo sapiens
15 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NDP  
Affinity Capture-MS Homo sapiens
17 PCDHB1  
Affinity Capture-MS Homo sapiens
18 TNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 C7orf34  
Affinity Capture-MS Homo sapiens
20 HNRNPCL3 649330
Cross-Linking-MS (XL-MS) Homo sapiens
21 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 GALNT6 11226
Affinity Capture-MS Homo sapiens
23 GALNT10 55568
Affinity Capture-MS Homo sapiens
24 FAM19A4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TMEM30B 161291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ADAM32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 LGALS1 3956
Affinity Capture-MS Homo sapiens
28 RLN1  
Affinity Capture-MS Homo sapiens
29 CLEC12B  
Affinity Capture-MS Homo sapiens
30 CLEC4A  
Affinity Capture-MS Homo sapiens
31 ITFG3 83986
Affinity Capture-MS Homo sapiens
32 HNRNPCL1 343069
Cross-Linking-MS (XL-MS) Homo sapiens
33 XAB2 56949
Co-fractionation Homo sapiens
34 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SLAMF1 6504
Affinity Capture-MS Homo sapiens
37 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 IFT80 57560
Two-hybrid Homo sapiens
39 HNRNPCL2 440563
Cross-Linking-MS (XL-MS) Homo sapiens
40 UBAP2L 9898
Two-hybrid Homo sapiens
41 KIR3DL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 EDDM3A  
Affinity Capture-MS Homo sapiens
43 DEFB136  
Affinity Capture-MS Homo sapiens
44 IGHD  
Affinity Capture-MS Homo sapiens
45 ADAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
47 LACRT 90070
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SUSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 C1QA 712
Affinity Capture-MS Homo sapiens
50 FBXO2 26232
Affinity Capture-MS Homo sapiens
51 PATE1  
Affinity Capture-MS Homo sapiens
52 CD1E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 C1orf54  
Affinity Capture-MS Homo sapiens
54 DEFA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 DEFB135  
Affinity Capture-MS Homo sapiens
56 HFE  
Affinity Capture-MS Homo sapiens
57 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 BRICD5  
Affinity Capture-MS Homo sapiens
59 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 DEFB109P1B  
Affinity Capture-MS Homo sapiens
61 HYOU1 10525
Affinity Capture-MS Homo sapiens
62 KRAS 3845
Proximity Label-MS Homo sapiens
63 MPPE1 65258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CEMIP 57214
Two-hybrid Homo sapiens
65 KLRC1  
Affinity Capture-MS Homo sapiens
66 GPHA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ST14 6768
Affinity Capture-MS Homo sapiens
68 BTNL3  
Affinity Capture-MS Homo sapiens
69 SPINK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CANX 821
Affinity Capture-MS Homo sapiens
71 LYPD2  
Affinity Capture-MS Homo sapiens
72 CST9L  
Affinity Capture-MS Homo sapiens
73 EDDM3B  
Affinity Capture-MS Homo sapiens
74 IGFL3  
Affinity Capture-MS Homo sapiens
75 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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