Gene description for NLE1
Gene name notchless homolog 1 (Drosophila)
Gene symbol NLE1
Other names/aliases Nle
Species Homo sapiens
 Database cross references - NLE1
ExoCarta ExoCarta_54475
Vesiclepedia VP_54475
Entrez Gene 54475
HGNC 19889
UniProt Q9NVX2  
 NLE1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NLE1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitotic cell cycle GO:0000278 IEA
    somitogenesis GO:0001756 IEA
    kidney development GO:0001822 IEA
    inner cell mass cell differentiation GO:0001826 IEA
    Notch signaling pathway GO:0007219 IEA
    regulation of Notch signaling pathway GO:0008593 IBA
    ribosomal large subunit biogenesis GO:0042273 IEA
    negative regulation of mitotic cell cycle GO:0045930 IEA
    skeletal system morphogenesis GO:0048705 IEA
    hematopoietic stem cell homeostasis GO:0061484 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified NLE1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NLE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 CNBP 7555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 PRM2  
Affinity Capture-MS Homo sapiens
5 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 WDR5 11091
Affinity Capture-Western Homo sapiens
8 TMPO 7112
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
9 DNAJB6 10049
Proximity Label-MS Homo sapiens
10 RPL30 6156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NOP9 161424
Affinity Capture-MS Homo sapiens
12 SIRT7  
Affinity Capture-MS Homo sapiens
13 SRPK3  
Affinity Capture-MS Homo sapiens
14 CCT6A 908
Affinity Capture-MS Homo sapiens
15 RPA2 6118
Proximity Label-MS Homo sapiens
16 RPL13A 23521
Affinity Capture-MS Homo sapiens
17 NLE1 54475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NPM3 10360
Affinity Capture-MS Homo sapiens
19 USP36  
Affinity Capture-MS Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 RPL15 6138
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SRSF5 6430
Affinity Capture-MS Homo sapiens
23 RPL14 9045
Affinity Capture-MS Homo sapiens
24 CCT4 10575
Affinity Capture-MS Homo sapiens
25 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PLEKHO1 51177
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NSA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 H2AFB3  
Affinity Capture-MS Homo sapiens
29 MTHFD1 4522
Co-fractionation Homo sapiens
30 DDX58 23586
Affinity Capture-RNA Homo sapiens
31 SRSF3 6428
Affinity Capture-MS Homo sapiens
32 NIFK 84365
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CCT7 10574
Affinity Capture-MS Homo sapiens
34 CCT5 22948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 THAP7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 PURG  
Affinity Capture-MS Homo sapiens
37 Spire2  
Affinity Capture-MS Mus musculus
38 RBM4 5936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 GPATCH4 54865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SPAG7  
Co-fractionation Homo sapiens
41 ADARB1 104
Affinity Capture-MS Homo sapiens
42 CHMP4B 128866
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 IGHMBP2 3508
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 LLPH  
Affinity Capture-MS Homo sapiens
46 HECTD1 25831
Affinity Capture-MS Homo sapiens
47 DNAJC9 23234
Proximity Label-MS Homo sapiens
48 NOL12 79159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CHRM5  
Two-hybrid Homo sapiens
50 PARK2  
Affinity Capture-MS Homo sapiens
51 RPL28 6158
Affinity Capture-MS Homo sapiens
52 GBE1 2632
Co-fractionation Homo sapiens
53 SKIV2L2 23517
Co-fractionation Homo sapiens
54 RBM4B  
Affinity Capture-MS Homo sapiens
55 RPL18 6141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 LYAR 55646
Affinity Capture-MS Homo sapiens
57 CYCS 54205
Co-fractionation Homo sapiens
58 NPM1 4869
Affinity Capture-MS Homo sapiens
59 H2AFY2 55506
Affinity Capture-MS Homo sapiens
60 H2AFB2  
Affinity Capture-MS Homo sapiens
61 OBSL1 23363
Affinity Capture-MS Homo sapiens
62 EWSR1 2130
Two-hybrid Homo sapiens
63 CCND1 595
Affinity Capture-MS Homo sapiens
64 MAGEB2 4113
Affinity Capture-MS Homo sapiens
65 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
66 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
67 KRAS 3845
Negative Genetic Homo sapiens
68 PRKRA 8575
Affinity Capture-MS Homo sapiens
69 GNL3 26354
Affinity Capture-MS Homo sapiens
70 PNMAL1  
Affinity Capture-MS Homo sapiens
71 HNRNPH3 3189
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NLE1 is involved
No pathways found





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