Gene description for PPP3CC
Gene name protein phosphatase 3, catalytic subunit, gamma isozyme
Gene symbol PPP3CC
Other names/aliases CALNA3
CNA3
PP2Bgamma
Species Homo sapiens
 Database cross references - PPP3CC
ExoCarta ExoCarta_5533
Vesiclepedia VP_5533
Entrez Gene 5533
HGNC 9316
MIM 114107
UniProt P48454  
 PPP3CC identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPP3CC
Molecular Function
    calcium-dependent protein serine/threonine phosphatase activity GO:0004723 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    myosin phosphatase activity GO:0017018 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IBA
    calmodulin-dependent protein phosphatase activity GO:0033192 IDA
    metal ion binding GO:0046872 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 IDA
    calcineurin-NFAT signaling cascade GO:0033173 IDA
    positive regulation of calcineurin-NFAT signaling cascade GO:0070886 NAS
    calcineurin-mediated signaling GO:0097720 IBA
    positive regulation of synaptic vesicle endocytosis GO:1900244 IEA
    positive regulation of calcium ion import across plasma membrane GO:1905665 NAS
    negative regulation of calcium ion import across plasma membrane GO:1905949 NAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    calcineurin complex GO:0005955 IBA
    calcineurin complex GO:0005955 NAS
    protein serine/threonine phosphatase complex GO:0008287 NAS
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified PPP3CC in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPP3CC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDC2 6383
Two-hybrid Homo sapiens
2 C16orf74  
Affinity Capture-MS Homo sapiens
3 TNS4 84951
Affinity Capture-MS Homo sapiens
4 STARD8  
Affinity Capture-MS Homo sapiens
5 PI4KA 5297
Affinity Capture-MS Homo sapiens
6 HAX1  
Two-hybrid Homo sapiens
7 CRTC2 200186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PPP3R1 5534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 DGCR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ARMT1 79624
Co-fractionation Homo sapiens
12 CSNK2B 1460
Two-hybrid Homo sapiens
13 PARP1 142
Proximity Label-MS Homo sapiens
14 MAP7 9053
Affinity Capture-MS Homo sapiens
15 SIK3 23387
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GUSBP5  
Affinity Capture-MS Homo sapiens
19 ASIP  
Affinity Capture-MS Homo sapiens
20 CSN2 1447
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 GSK3A 2931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 KCNK18  
Affinity Capture-MS Homo sapiens
23 CALM2 805
Affinity Capture-MS Homo sapiens
24 PMEPA1 56937
Affinity Capture-MS Homo sapiens
25 NFATC1 4772
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TMCC3  
Affinity Capture-MS Homo sapiens
27 FAM163B  
Affinity Capture-MS Homo sapiens
28 PRKAR2A  
Biochemical Activity Bos taurus
29 AMMECR1  
Affinity Capture-MS Homo sapiens
30 ITPKC  
Two-hybrid Homo sapiens
31 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MLH1 4292
Affinity Capture-MS Homo sapiens
33 RITA1  
Affinity Capture-MS Homo sapiens
34 PARD3B 117583
Affinity Capture-MS Homo sapiens
35 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ARID3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ARG1 383
Affinity Capture-MS Homo sapiens
38 CALM1 801
Affinity Capture-MS Homo sapiens
39 APP 351
Reconstituted Complex Homo sapiens
40 TMEM200A 114801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 THAP7  
Two-hybrid Homo sapiens
42 AMPH 273
Two-hybrid Homo sapiens
43 PPP3R2  
Affinity Capture-MS Homo sapiens
44 HOXC5  
Affinity Capture-MS Homo sapiens
45 PRR3  
Affinity Capture-MS Homo sapiens
46 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 LBH  
Affinity Capture-MS Homo sapiens
48 ARHGAP44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 BCL2  
Reconstituted Complex Homo sapiens
50 SRBD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SPATA33  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
52 KSR2  
Affinity Capture-MS Homo sapiens
53 TIMMDC1  
Affinity Capture-MS Homo sapiens
54 PPP3CB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CDR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 UNC119 9094
Two-hybrid Homo sapiens
57 RCAN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 CALM3 808
Affinity Capture-MS Homo sapiens
59 LRRC41 10489
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 IGFBP6 3489
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 HGFAC 3083
Affinity Capture-MS Homo sapiens
62 RCAN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RCAN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 NFATC4 4776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 HYPM  
Affinity Capture-MS Homo sapiens
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