Gene description for ARHGAP22
Gene name Rho GTPase activating protein 22
Gene symbol ARHGAP22
Other names/aliases RhoGAP2
RhoGap22
Species Homo sapiens
 Database cross references - ARHGAP22
ExoCarta ExoCarta_58504
Vesiclepedia VP_58504
Entrez Gene 58504
HGNC 30320
MIM 610585
UniProt Q7Z5H3  
 ARHGAP22 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGAP22
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
Biological Process
    angiogenesis GO:0001525 IEA
    signal transduction GO:0007165 IEA
    cell differentiation GO:0030154 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    negative regulation of small GTPase mediated signal transduction GO:0051058 IBA
    regulation of postsynapse organization GO:0099175 IBA
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 HDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified ARHGAP22 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGAP22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MECP2 4204
Affinity Capture-MS Homo sapiens
3 ARRB2 409
Affinity Capture-MS Homo sapiens
4 CCNDBP1 23582
Two-hybrid Homo sapiens
5 PPP3R1 5534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 RCN1 5954
Affinity Capture-MS Homo sapiens
8 RPS29 6235
Affinity Capture-MS Homo sapiens
9 IBA57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 JUND 3727
Affinity Capture-MS Homo sapiens
11 FGD3  
Affinity Capture-MS Homo sapiens
12 CCDC86  
Affinity Capture-MS Homo sapiens
13 PDCD2L 84306
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ARMC8 25852
Affinity Capture-MS Homo sapiens
15 CALU 813
Affinity Capture-MS Homo sapiens
16 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TIMM50 92609
Affinity Capture-MS Homo sapiens
18 NUP62 23636
Affinity Capture-MS Homo sapiens
19 DPY30 84661
Affinity Capture-MS Homo sapiens
20 PLCD3 113026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CACNA1A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
23 CSTF2 1478
Affinity Capture-MS Homo sapiens
24 KDM6A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SSBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 KPNA2 3838
Affinity Capture-MS Homo sapiens
27 RBM6 10180
Affinity Capture-MS Homo sapiens
28 CREB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 DDX58 23586
Affinity Capture-RNA Homo sapiens
30 ATF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PLD1 5337
Affinity Capture-MS Homo sapiens
32 AGAP3 116988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TFCP2 7024
Affinity Capture-MS Homo sapiens
34 CDC42 998
FRET Homo sapiens
35 ATF2  
Affinity Capture-MS Homo sapiens
36 JUN 3725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TRIM37  
Proximity Label-MS Homo sapiens
39 CSNK1D 1453
Affinity Capture-MS Homo sapiens
40 GAR1 54433
Affinity Capture-MS Homo sapiens
41 SRBD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CIRBP 1153
Affinity Capture-MS Homo sapiens
43 SEC16A 9919
Affinity Capture-MS Homo sapiens
44 TRIM27  
Affinity Capture-MS Homo sapiens
45 COL10A1  
Affinity Capture-MS Homo sapiens
46 MAEA 10296
Affinity Capture-MS Homo sapiens
47 PPP6C 5537
Affinity Capture-MS Homo sapiens
48 CSNK1E 1454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ANK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RAC1 5879
FRET Homo sapiens
52 PSPC1 55269
Affinity Capture-MS Homo sapiens
53 DOCK11 139818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RHOA 387
FRET Homo sapiens
55 ERI3 79033
Affinity Capture-MS Homo sapiens
56 PABPN1 8106
Affinity Capture-MS Homo sapiens
57 RAE1 8480
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DAXX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PPP3CC 5533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here