Gene description for CHST12
Gene name carbohydrate (chondroitin 4) sulfotransferase 12
Gene symbol CHST12
Other names/aliases C4S-2
C4ST-2
C4ST2
Species Homo sapiens
 Database cross references - CHST12
ExoCarta ExoCarta_55501
Vesiclepedia VP_55501
Entrez Gene 55501
HGNC 17423
MIM 610129
UniProt Q9NRB3  
 CHST12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CHST12
Molecular Function
    sulfotransferase activity GO:0008146 IBA
    chondroitin 4-sulfotransferase activity GO:0047756 IDA
    chondroitin 4-sulfotransferase activity GO:0047756 TAS
    3'-phosphoadenosine 5'-phosphosulfate binding GO:0050656 NAS
Biological Process
    carbohydrate biosynthetic process GO:0016051 IEA
    proteoglycan biosynthetic process GO:0030166 IBA
    chondroitin sulfate biosynthetic process GO:0030206 IDA
    chondroitin sulfate biosynthetic process GO:0030206 TAS
    dermatan sulfate biosynthetic process GO:0030208 IDA
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified CHST12 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CHST12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 B4GAT1 11041
Affinity Capture-MS Homo sapiens
2 GAA 2548
Affinity Capture-MS Homo sapiens
3 Gspt1 14852
Affinity Capture-MS Mus musculus
4 DKK3 27122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HLA-G 3135
Affinity Capture-MS Homo sapiens
6 HSPA5 3309
Affinity Capture-MS Homo sapiens
7 BTNL2  
Affinity Capture-MS Homo sapiens
8 TMEM106A  
Affinity Capture-MS Homo sapiens
9 NAAA  
Affinity Capture-MS Homo sapiens
10 KLK15  
Affinity Capture-MS Homo sapiens
11 CEACAM8 1088
Affinity Capture-MS Homo sapiens
12 LGI1  
Affinity Capture-MS Homo sapiens
13 CLGN 1047
Affinity Capture-MS Homo sapiens
14 TMEM59 9528
Affinity Capture-MS Homo sapiens
15 MAD2L2 10459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CANX 821
Affinity Capture-MS Homo sapiens
17 LCN6  
Affinity Capture-MS Homo sapiens
18 UGGT1 56886
Affinity Capture-MS Homo sapiens
19 C1orf54  
Affinity Capture-MS Homo sapiens
20 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C7orf34  
Affinity Capture-MS Homo sapiens
22 ALPPL2 251
Affinity Capture-MS Homo sapiens
23 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ITM2C 81618
Affinity Capture-MS Homo sapiens
25 DPEP2  
Affinity Capture-MS Homo sapiens
26 PDGFRA 5156
Affinity Capture-MS Homo sapiens
27 PRG2  
Affinity Capture-MS Homo sapiens
28 MFAP4 4239
Affinity Capture-MS Homo sapiens
29 ECEL1  
Affinity Capture-MS Homo sapiens
30 LDLRAD1  
Affinity Capture-MS Homo sapiens
31 RLN1  
Affinity Capture-MS Homo sapiens
32 CBLN4  
Affinity Capture-MS Homo sapiens
33 CANT1 124583
Affinity Capture-MS Homo sapiens
34 RMND1 55005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 XRCC6BP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 HLA-DPA1  
Affinity Capture-MS Homo sapiens
38 SFTPC  
Affinity Capture-MS Homo sapiens
39 CLEC12B  
Affinity Capture-MS Homo sapiens
40 CHRNB2  
Affinity Capture-MS Homo sapiens
41 C1QTNF7  
Affinity Capture-MS Homo sapiens
42 SDF2L1 23753
Affinity Capture-MS Homo sapiens
43 C16orf58 64755
Affinity Capture-MS Homo sapiens
44 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SCGB2A2  
Affinity Capture-MS Homo sapiens
46 CBX8 57332
Affinity Capture-MS Homo sapiens
47 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DNAJB9 4189
Affinity Capture-MS Homo sapiens
50 GXYLT1 283464
Affinity Capture-MS Homo sapiens
51 PTPRK 5796
Affinity Capture-MS Homo sapiens
52 SEL1L 6400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 B3GAT3 26229
Affinity Capture-MS Homo sapiens
54 Mzt2  
Affinity Capture-MS Mus musculus
55 IFI30  
Affinity Capture-MS Homo sapiens
56 ST8SIA5  
Affinity Capture-MS Homo sapiens
57 ISLR 3671
Affinity Capture-MS Homo sapiens
58 TNFSF13B  
Affinity Capture-MS Homo sapiens
59 SSRP1 6749
Affinity Capture-MS Homo sapiens
60 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SDF4 51150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CHRNA4  
Affinity Capture-MS Homo sapiens
63 MPPE1 65258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 EDDM3B  
Affinity Capture-MS Homo sapiens
65 FIBIN  
Affinity Capture-MS Homo sapiens
66 LIPG 9388
Affinity Capture-MS Homo sapiens
67 ITM2B 9445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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