Gene description for PRKCQ
Gene name protein kinase C, theta
Gene symbol PRKCQ
Other names/aliases PRKCT
nPKC-theta
Species Homo sapiens
 Database cross references - PRKCQ
ExoCarta ExoCarta_5588
Vesiclepedia VP_5588
Entrez Gene 5588
HGNC 9410
MIM 600448
UniProt Q04759  
 PRKCQ identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PRKCQ
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 NAS
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 EXP
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    regulation of cell growth GO:0001558 NAS
    regulation of DNA-templated transcription GO:0006355 ISS
    protein phosphorylation GO:0006468 IDA
    membrane protein ectodomain proteolysis GO:0006509 ISS
    inflammatory response GO:0006954 IEA
    axon guidance GO:0007411 TAS
    positive regulation of telomere maintenance GO:0032206 IMP
    positive regulation of interleukin-17 production GO:0032740 ISS
    positive regulation of interleukin-2 production GO:0032743 IEA
    positive regulation of interleukin-4 production GO:0032753 ISS
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 NAS
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    positive regulation of T cell activation GO:0050870 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    cell chemotaxis GO:0060326 IMP
    negative regulation of T cell apoptotic process GO:0070233 IMP
    regulation of platelet aggregation GO:0090330 ISS
    positive regulation of T-helper 17 type immune response GO:2000318 ISS
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 IEA
    positive regulation of T-helper 2 cell activation GO:2000570 ISS
Subcellular Localization
    immunological synapse GO:0001772 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    aggresome GO:0016235 IDA
    centriolar satellite GO:0034451 IDA
 Experiment description of studies that identified PRKCQ in sEVs
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKCQ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C2CD2L 9854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 LCK 3932
Two-hybrid Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 ZNF24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MTCH1 23787
Proximity Label-MS Homo sapiens
6 RASGRP3  
Biochemical Activity Homo sapiens
7 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PRKCD 5580
Affinity Capture-MS Homo sapiens
9 JUND 3727
Affinity Capture-Western Homo sapiens
10 LAMP3  
Proximity Label-MS Homo sapiens
11 AKT1 207
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 TAL1  
Two-hybrid Homo sapiens
13 IKBKG 8517
Affinity Capture-Western Homo sapiens
14 C19orf66  
Affinity Capture-MS Homo sapiens
15 VAV1 7409
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
17 CARD11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
18 LAMP2 3920
Proximity Label-MS Homo sapiens
19 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
20 PDPK1 5170
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 FYB 2533
Affinity Capture-Western Homo sapiens
23 CBL 867
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
24 PRKCQ 5588
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
25 RAB2A 5862
Proximity Label-MS Homo sapiens
26 FYN 2534
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 PELI1 57162
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
29 BCL10  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 YWHAQ 10971
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
31 CCNA2 890
Affinity Capture-MS Homo sapiens
32 GLRX3 10539
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
33 MALT1 10892
Affinity Capture-Western Homo sapiens
34 YWHAG 7532
Affinity Capture-Western Homo sapiens
35 FKBP5 2289
Affinity Capture-MS Homo sapiens
36 SELL 6402
Reconstituted Complex Homo sapiens
37 IKBKB 3551
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 CHUK 1147
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 AKAP1 8165
Proximity Label-MS Homo sapiens
40 KCNA5  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which PRKCQ is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Apoptosis TAS Reactome
Apoptotic cleavage of cellular proteins TAS Reactome
Apoptotic execution phase TAS Reactome
Axon guidance TAS Reactome
Developmental Biology TAS Reactome
Downstream TCR signaling TAS Reactome
Effects of PIP2 hydrolysis TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation TAS Reactome
FCERI mediated NF-kB activation IEA Reactome
G alpha (q) signalling events TAS Reactome
G alpha (z) signalling events IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
GPCR downstream signalling IEA Reactome
GPCR downstream signalling TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Inactivation, recovery and regulation of the phototransduction cascade IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Nervous system development TAS Reactome
Netrin-1 signaling TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Programmed Cell Death TAS Reactome
RAF/MAP kinase cascade TAS Reactome
RAS processing TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function TAS Reactome
Sensory Perception IEA Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by GPCR TAS Reactome
TCR signaling TAS Reactome
The phototransduction cascade IEA Reactome
Transcriptional regulation by RUNX1 TAS Reactome
Visual phototransduction IEA Reactome





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