Gene description for RFX3
Gene name regulatory factor X, 3 (influences HLA class II expression)
Gene symbol RFX3
Other names/aliases -
Species Homo sapiens
 Database cross references - RFX3
ExoCarta ExoCarta_5991
Vesiclepedia VP_5991
Entrez Gene 5991
HGNC 9984
MIM 601337
UniProt P48380  
 RFX3 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RFX3
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA binding GO:0003677 TAS
    DNA-binding transcription factor activity GO:0003700 ISS
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    DNA-templated transcription GO:0006351 IEA
    regulation of DNA-templated transcription GO:0006355 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    endocrine pancreas development GO:0031018 ISS
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    cell maturation GO:0048469 ISS
    regulation of insulin secretion GO:0050796 ISS
    cilium assembly GO:0060271 ISS
    cilium-dependent cell motility GO:0060285 ISS
    epithelial cilium movement involved in determination of left/right asymmetry GO:0060287 ISS
    type B pancreatic cell maturation GO:0072560 IEA
    positive regulation of type B pancreatic cell development GO:2000078 ISS
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISA
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 IC
    transcription regulator complex GO:0005667 ISS
 Experiment description of studies that identified RFX3 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RFX3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Affinity Capture-MS Homo sapiens
2 TMED4 222068
Affinity Capture-MS Homo sapiens
3 TRIP6 7205
Two-hybrid Homo sapiens
4 SEC63 11231
Affinity Capture-MS Homo sapiens
5 PKNOX1  
Two-hybrid Homo sapiens
6 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 MTA2 9219
Affinity Capture-MS Homo sapiens
8 RFX4  
Affinity Capture-Western Homo sapiens
9 RFX6  
Two-hybrid Homo sapiens
10 FOXJ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MTCH2 23788
Affinity Capture-MS Homo sapiens
12 FHL3 2275
Two-hybrid Homo sapiens
13 VPS45 11311
Affinity Capture-MS Homo sapiens
14 RFX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HDAC1 3065
Affinity Capture-MS Homo sapiens
16 CREB1  
Two-hybrid Homo sapiens
17 MKI67  
Affinity Capture-MS Homo sapiens
18 SOX2  
Affinity Capture-MS Homo sapiens
19 PTOV1  
Affinity Capture-MS Homo sapiens
20 FOXJ2  
Affinity Capture-MS Homo sapiens
21 INPP5K 51763
Affinity Capture-MS Homo sapiens
22 TBC1D32 221322
Affinity Capture-MS Homo sapiens
23 ACAD9 28976
Affinity Capture-MS Homo sapiens
24 RFX2  
Affinity Capture-MS Homo sapiens
25 RTCB 51493
Affinity Capture-MS Homo sapiens
26 PABPC5 140886
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 IGSF8 93185
Affinity Capture-MS Homo sapiens
28 FOXN3 1112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PLK1 5347
Affinity Capture-MS Homo sapiens
30 HDAC2 3066
Affinity Capture-MS Homo sapiens
31 CDK20  
Affinity Capture-MS Homo sapiens
32 MBD2 8932
Affinity Capture-MS Homo sapiens
33 DDX1 1653
Affinity Capture-MS Homo sapiens
34 FAM188A 80013
Affinity Capture-MS Homo sapiens
35 EWSR1 2130
Two-hybrid Homo sapiens
36 IRF6 3664
Two-hybrid Homo sapiens
37 XYLT2 64132
Affinity Capture-MS Homo sapiens
38 FHL2 2274
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
39 BIN1 274
Two-hybrid Homo sapiens
40 TAF6L 10629
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RFX3 is involved
No pathways found





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