Gene description for RNF25
Gene name ring finger protein 25
Gene symbol RNF25
Other names/aliases AO7
Species Homo sapiens
 Database cross references - RNF25
ExoCarta ExoCarta_64320
Vesiclepedia VP_64320
Entrez Gene 64320
HGNC 14662
MIM 616014
UniProt Q96BH1  
 RNF25 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RNF25
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    NF-kappaB binding GO:0051059 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein ubiquitination GO:0016567 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    rescue of stalled ribosome GO:0072344 IBA
    rescue of stalled ribosome GO:0072344 IDA
    protein K6-linked ubiquitination GO:0085020 IDA
    protein-RNA covalent cross-linking repair GO:0160127 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IDA
    cytosolic ribosome GO:0022626 IDA
 Experiment description of studies that identified RNF25 in sEVs
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RNF25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRP19 6728
Affinity Capture-MS Homo sapiens
2 RELA 5970
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 SERPINB4 6318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 IVL 3713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 Ube2e3  
Reconstituted Complex Mus musculus
7 NKD2 85409
Affinity Capture-Western Homo sapiens
8 UBE2E3 10477
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
9 RNF25 64320
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
10 UBE2L6 9246
Two-hybrid Homo sapiens
11 IGKC 3514
Affinity Capture-MS Homo sapiens
12 SCFD1 23256
Affinity Capture-MS Homo sapiens
13 UCN3  
Affinity Capture-MS Homo sapiens
14 S100A7 6278
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBC 7316
Affinity Capture-MS Homo sapiens
16 UBE2E1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RBFOX1 54715
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 UBE2D4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MAPK1 5594
Co-fractionation Homo sapiens
20 SLC2A7  
Affinity Capture-MS Homo sapiens
21 UBE2D2 7322
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
22 CMTR1 23070
Affinity Capture-MS Homo sapiens
23 UBE2E2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HTATSF1 27336
Affinity Capture-MS Homo sapiens
25 TRIM27  
Two-hybrid Homo sapiens
26 HSPA1A 3303
Affinity Capture-MS Homo sapiens
27 MEOX2  
Two-hybrid Homo sapiens
28 KCTD21 283219
Two-hybrid Homo sapiens
29 UBE2D3 7323
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 DFFA 1676
Co-fractionation Homo sapiens
31 STX5 6811
Affinity Capture-MS Homo sapiens
32 FAM188A 80013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 STX18 53407
Affinity Capture-MS Homo sapiens
34 UBE2U  
Two-hybrid Homo sapiens
35 TULP3 7289
Affinity Capture-MS Homo sapiens
36 LCN2 3934
Affinity Capture-MS Homo sapiens
37 UBE2D1 7321
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
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