Gene description for ST6GAL1
Gene name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Gene symbol ST6GAL1
Other names/aliases SIAT1
ST6GalI
ST6N
Species Homo sapiens
 Database cross references - ST6GAL1
ExoCarta ExoCarta_6480
Vesiclepedia VP_6480
Entrez Gene 6480
HGNC 10860
MIM 109675
UniProt P15907  
 ST6GAL1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ST6GAL1
Molecular Function
    beta-galactoside alpha-2,6-sialyltransferase activity GO:0003835 IBA
    beta-galactoside alpha-2,6-sialyltransferase activity GO:0003835 IDA
    beta-galactoside alpha-2,6-sialyltransferase activity GO:0003835 TAS
    protein binding GO:0005515 IPI
    sialyltransferase activity GO:0008373 IDA
    sialyltransferase activity GO:0008373 TAS
    protein homodimerization activity GO:0042803 IDA
Biological Process
    N-acetylneuraminate metabolic process GO:0006054 IDA
    humoral immune response GO:0006959 TAS
    O-glycan processing GO:0016266 TAS
    protein N-linked glycosylation via asparagine GO:0018279 IBA
    protein N-linked glycosylation via asparagine GO:0018279 IDA
    viral protein processing GO:0019082 TAS
    positive regulation of mononuclear cell proliferation GO:0032946 IEA
    response to ethanol GO:0045471 IEA
    negative regulation of chemotaxis GO:0050922 IEA
    sialylation GO:0097503 IBA
    sialylation GO:0097503 IDA
    regulation of substrate adhesion-dependent cell spreading GO:1900024 IEA
    negative regulation of macrophage apoptotic process GO:2000110 IEA
Subcellular Localization
    Golgi trans cisterna GO:0000138 IEA
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi medial cisterna GO:0005797 IEA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified ST6GAL1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ST6GAL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLAA 9373
Affinity Capture-MS Homo sapiens
2 KIAA1244  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GLMN 11146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TNPO3 23534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ATRIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PRMT9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 KIAA1524 57650
Affinity Capture-MS Homo sapiens
8 ERGIC2 51290
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 WAPAL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SAAL1 113174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 HEATR3 55027
Affinity Capture-MS Homo sapiens
13 ST6GAL1 6480
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 HLA-B 3106
Affinity Capture-MS Homo sapiens
15 HOOK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 INTS3 65123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 INTS12  
Affinity Capture-MS Homo sapiens
18 DDX58 23586
Affinity Capture-RNA Homo sapiens
19 ZYG11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 USP28 57646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HLA-C 3107
Affinity Capture-MS Homo sapiens
22 ATP2A1 487
Affinity Capture-MS Homo sapiens
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