Gene description for RNF113A
Gene name ring finger protein 113A
Gene symbol RNF113A
Other names/aliases Cwc24
RNF113
ZNF183
Species Homo sapiens
 Database cross references - RNF113A
ExoCarta ExoCarta_7737
Vesiclepedia VP_7737
Entrez Gene 7737
HGNC 12974
MIM 300951
UniProt O15541  
 RNF113A identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RNF113A
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IMP
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IDA
    DNA repair GO:0006281 IEA
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 IMP
    snoRNA splicing GO:0034247 IBA
    negative regulation of chemokine-mediated signaling pathway GO:0070100 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    U2-type spliceosomal complex GO:0005684 IBA
    nuclear speck GO:0016607 IEA
    U2-type precatalytic spliceosome GO:0071005 IDA
 Experiment description of studies that identified RNF113A in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RNF113A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 CCAR1 55749
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 PSMA6 5687
Co-fractionation Homo sapiens
6 RBM39 9584
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 TERF2  
Two-hybrid Homo sapiens
8 FEM1A  
Affinity Capture-MS Homo sapiens
9 CXCR4 7852
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 ILF3 3609
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 RBM8A 9939
Affinity Capture-MS Homo sapiens
12 TRIM8  
Two-hybrid Homo sapiens
13 EGLN3  
Affinity Capture-MS Homo sapiens
14 KPNB1 3837
Affinity Capture-MS Homo sapiens
15 ACTC1 70
Proximity Label-MS Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
17 RNF113B  
Affinity Capture-MS Homo sapiens
18 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 Rcc1  
Affinity Capture-MS Mus musculus
20 SF3B2 10992
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
21 APEX1 328
Proximity Label-MS Homo sapiens
22 PCDH7 5099
Affinity Capture-MS Homo sapiens
23 SNRNP200 23020
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 NUP50 10762
Proximity Label-MS Homo sapiens
25 IK 3550
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
26 RDX 5962
Affinity Capture-MS Homo sapiens
27 EIF3J 8669
Affinity Capture-MS Homo sapiens
28 C9orf41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 EIF4B 1975
Affinity Capture-MS Homo sapiens
30 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 AASDH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SRRM2 23524
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 UBE2D3 7323
Reconstituted Complex Homo sapiens
34 RNF113A 7737
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
35 POLR1E  
Proximity Label-MS Homo sapiens
36 RIF1  
Affinity Capture-MS Homo sapiens
37 LMNA 4000
Proximity Label-MS Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 CEBPA  
Proximity Label-MS Homo sapiens
40 COIL  
Proximity Label-MS Homo sapiens
41 UBE2U  
Two-hybrid Homo sapiens
42 ID2  
Affinity Capture-MS Homo sapiens
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