Gene description for NCALD
Gene name neurocalcin delta
Gene symbol NCALD
Other names/aliases -
Species Homo sapiens
 Database cross references - NCALD
ExoCarta ExoCarta_83988
Vesiclepedia VP_83988
Entrez Gene 83988
HGNC 7655
MIM 606722
UniProt P61601  
 NCALD identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 19837982    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NCALD
Molecular Function
    actin binding GO:0003779 IBA
    actin binding GO:0003779 IDA
    calcium ion binding GO:0005509 IBA
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 IBA
    tubulin binding GO:0015631 IDA
    clathrin binding GO:0030276 IDA
    alpha-tubulin binding GO:0043014 IEA
Biological Process
    regulation of systemic arterial blood pressure GO:0003073 IEA
    regulation of signal transduction GO:0009966 IBA
    vesicle-mediated transport GO:0016192 NAS
    calcium-mediated signaling GO:0019722 IBA
Subcellular Localization
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    clathrin coat of trans-Golgi network vesicle GO:0030130 NAS
 Experiment description of studies that identified NCALD in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCALD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MINOS1-NBL1  
Two-hybrid Homo sapiens
2 GPM6A 2823
Affinity Capture-MS Homo sapiens
3 C1QTNF2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 TMEM14B  
Two-hybrid Homo sapiens
5 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 VSNL1 7447
Affinity Capture-MS Homo sapiens
7 HRASLS2  
Two-hybrid Homo sapiens
8 PARP1 142
Affinity Capture-MS Homo sapiens
9 NBL1 4681
Two-hybrid Homo sapiens
10 SRGN 5552
Two-hybrid Homo sapiens
11 KRT6A 3853
Two-hybrid Homo sapiens
12 HPCAL1 3241
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
13 TPSG1  
Two-hybrid Homo sapiens
14 VTCN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C19orf44  
Two-hybrid Homo sapiens
17 LITAF 9516
Two-hybrid Homo sapiens
18 FGF6  
Two-hybrid Homo sapiens
19 TUBB4B 10383
Affinity Capture-MS Homo sapiens
20 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SBSN 374897
Two-hybrid Homo sapiens
22 SSX1  
Affinity Capture-MS Homo sapiens
23 GOLGA7 51125
Two-hybrid Homo sapiens
24 LY6G6D  
Two-hybrid Homo sapiens
25 IGFBP6 3489
Two-hybrid Homo sapiens
26 ARMC1 55156
Affinity Capture-MS Homo sapiens
27 APOL3  
Two-hybrid Homo sapiens
28 MIEF2  
Two-hybrid Homo sapiens
29 FZD7 8324
Two-hybrid Homo sapiens
30 METTL21A  
Affinity Capture-MS Homo sapiens
31 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DTX2 113878
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 FAM174A 345757
Affinity Capture-MS Homo sapiens
34 MEOX2  
Two-hybrid Homo sapiens
35 LIF 3976
Two-hybrid Homo sapiens
36 ACTB 60
Affinity Capture-Western Homo sapiens
37 HSPBAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 LCE2D  
Two-hybrid Homo sapiens
39 TPP2 7174
Affinity Capture-MS Homo sapiens
40 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 GPX7 2882
Two-hybrid Homo sapiens
42 SPRED1 161742
Two-hybrid Homo sapiens
43 ANKRD33B  
Affinity Capture-MS Homo sapiens
44 HPCA 3208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
45 SH3GL2  
Affinity Capture-MS Homo sapiens
46 KIAA1804  
Affinity Capture-MS Homo sapiens
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