Experiment description of studies that identified ZNF622 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for ZNF622

Protein Interactor ExoCarta ID Identification method PubMed Species 1 NPEPPS 9520 Co-fractionation Homo sapiens 2 PAXIP1   Affinity Capture-MS Homo sapiens 3 TMED5 50999 Affinity Capture-MS Homo sapiens 4 RPL10 6134 Affinity Capture-MS Homo sapiens 5 PTPN1 5770 Proximity Label-MS Homo sapiens 6 CCDC102B   Affinity Capture-MS Homo sapiens 7 APP 351 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 8 RDX 5962 Affinity Capture-MS Homo sapiens 9 NPM1 4869 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 LSG1   Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 11 SRP14 6727 Affinity Capture-MS Homo sapiens 12 SRP68 6730 Affinity Capture-MS Homo sapiens 13 Eif3a 13669 Affinity Capture-MS Mus musculus 14 RBM42   Affinity Capture-MS Homo sapiens 15 ERBB2 2064 Affinity Capture-MS Homo sapiens 16 YAF2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 FAM20C 56975 Affinity Capture-MS Homo sapiens 18 RBM8A 9939 Affinity Capture-MS Homo sapiens 19 MELK   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 20 UNC45A 55898 Affinity Capture-MS Homo sapiens 21 GARS 2617 Affinity Capture-MS Homo sapiens 22 POP1 10940 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 SRP9 6726 Affinity Capture-MS Homo sapiens 24 PARK2   Affinity Capture-MS Homo sapiens 25 RBM28 55131 Co-fractionation Homo sapiens 26 STAU1 6780 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 RPL5 6125 Affinity Capture-MS Homo sapiens 28 UBFD1 56061 Co-fractionation Homo sapiens 29 Srp72   Affinity Capture-MS Mus musculus 30 OGT 8473 Reconstituted Complex Homo sapiens 31 FAM24B   Affinity Capture-MS Homo sapiens 32 BEND7 222389 Two-hybrid Homo sapiens 33 DCTN1 1639 Proximity Label-MS Homo sapiens 34 MYBL2 4605 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 35 HECTD1 25831 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 SRPK3   Affinity Capture-MS Homo sapiens 37 H2AFY 9555 Two-hybrid Homo sapiens 38 SEC61B 10952 Proximity Label-MS Homo sapiens 39 EIF2B4 8890 Co-fractionation Homo sapiens 40 PIPSL 266971 Affinity Capture-MS Homo sapiens 41 LOC388882   Affinity Capture-MS Homo sapiens 42 RPL11 6135 Affinity Capture-MS Homo sapiens 43 DNAJC21   Affinity Capture-MS Homo sapiens 44 NOC3L 64318 Affinity Capture-MS Homo sapiens 45 MRTO4 51154 Co-fractionation Homo sapiens 46 LRRC59 55379 Proximity Label-MS Homo sapiens 47 EIF6 3692 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 DNAJC2 27000 Proximity Label-MS Homo sapiens 49 ZNF622 90441 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 50 WDR33 55339 Co-fractionation Homo sapiens 51 BRIX1 55299 Affinity Capture-Western Homo sapiens 52 ATP5F1 515 Affinity Capture-MS Homo sapiens 53 STIM1 6786 Proximity Label-MS Homo sapiens 54 TUBB4B 10383 Affinity Capture-MS Homo sapiens 55 EBNA1BP2 10969 Affinity Capture-Western Homo sapiens 56 G6PD 2539 Co-fractionation Homo sapiens 57 EFTUD2 9343 Co-fractionation Homo sapiens 58 PCBP1 5093 Affinity Capture-MS Homo sapiens 59 PSPC1 55269 Affinity Capture-MS Homo sapiens 60 RPA3 6119 Proximity Label-MS Homo sapiens 61 AHR 196 Affinity Capture-MS Homo sapiens 62 NMD3 51068 Affinity Capture-MS Homo sapiens 63 DDRGK1 65992 Affinity Capture-MS Homo sapiens 64 GLA 2717 Co-fractionation Homo sapiens 65 Rpl35 66489 Affinity Capture-MS Mus musculus 66 SCLY   Co-fractionation Homo sapiens 67 Eif3e 16341 Affinity Capture-MS Mus musculus 68 BMP4 652 Affinity Capture-MS Homo sapiens 69 FTSJ3 117246 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 OAS3 4940 Affinity Capture-MS Homo sapiens 71 RPS16 6217 Affinity Capture-MS Homo sapiens 72 FTL 2512 Affinity Capture-MS Homo sapiens 73 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 74 FASN 2194 Positive Genetic Homo sapiens 75 Melk   Two-hybrid Mus musculus Affinity Capture-Western Mus musculus Biochemical Activity Mus musculus 76 FBXL6   Proximity Label-MS Homo sapiens 77 EZR 7430 Affinity Capture-MS Homo sapiens 78 C1orf35   Affinity Capture-MS Homo sapiens 79 SRP72 6731 Affinity Capture-MS Homo sapiens 80 DDX58 23586 Affinity Capture-RNA Homo sapiens 81 PRPF31 26121 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 82 PCDHA9   Affinity Capture-MS Homo sapiens 83 UFL1 23376 Affinity Capture-MS Homo sapiens 84 KRR1 11103 Affinity Capture-Western Homo sapiens 85 PPP1R2 5504 Co-fractionation Homo sapiens 86 RPL23AP32 56969 Affinity Capture-MS Homo sapiens 87 Rrbp1   Affinity Capture-MS Mus musculus 88 DNAJC1 64215 Proximity Label-MS Homo sapiens 89 HNRNPU 3192 Affinity Capture-MS Homo sapiens 90 GNB2L1 10399 Affinity Capture-MS Homo sapiens 91 CKAP4 10970 Proximity Label-MS Homo sapiens 92 PTRF 284119 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 93 NEMF 9147 Affinity Capture-MS Homo sapiens View the network

Pathways in which ZNF622 is involved

No pathways found