Gene description for SLIT2
Gene name slit homolog 2 (Drosophila)
Gene symbol SLIT2
Other names/aliases SLIL3
Slit-2
Species Homo sapiens
 Database cross references - SLIT2
ExoCarta ExoCarta_9353
Vesiclepedia VP_9353
Entrez Gene 9353
HGNC 11086
MIM 603746
UniProt O94813  
 SLIT2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for SLIT2
Molecular Function
    GTPase inhibitor activity GO:0005095 IDA
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IBA
    heparin binding GO:0008201 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    laminin-1 binding GO:0043237 IDA
    proteoglycan binding GO:0043394 IPI
    Roundabout binding GO:0048495 IBA
    Roundabout binding GO:0048495 IPI
Biological Process
    ureteric bud development GO:0001657 IMP
    negative regulation of protein phosphorylation GO:0001933 IDA
    cell migration involved in sprouting angiogenesis GO:0002042 IMP
    negative regulation of leukocyte chemotaxis GO:0002689 IDA
    aortic valve morphogenesis GO:0003180 ISS
    pulmonary valve morphogenesis GO:0003184 ISS
    axon guidance GO:0007411 IDA
    motor neuron axon guidance GO:0008045 IDA
    negative regulation of lamellipodium assembly GO:0010593 IDA
    negative regulation of endothelial cell migration GO:0010596 IDA
    negative regulation of smooth muscle cell migration GO:0014912 IDA
    chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration GO:0021834 IDA
    chemorepulsion involved in postnatal olfactory bulb interneuron migration GO:0021836 IDA
    corticospinal neuron axon guidance through spinal cord GO:0021972 IMP
    negative regulation of cell growth GO:0030308 IBA
    negative regulation of cell growth GO:0030308 IMP
    negative regulation of cell migration GO:0030336 IDA
    negative regulation of cell migration GO:0030336 IMP
    negative regulation of actin filament polymerization GO:0030837 IDA
    retinal ganglion cell axon guidance GO:0031290 IBA
    retinal ganglion cell axon guidance GO:0031290 IDA
    cellular response to hormone stimulus GO:0032870 IEP
    Roundabout signaling pathway GO:0035385 IC
    Roundabout signaling pathway GO:0035385 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of vascular permeability GO:0043116 IDA
    branching morphogenesis of an epithelial tube GO:0048754 IDA
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    axon extension involved in axon guidance GO:0048846 IDA
    positive regulation of axonogenesis GO:0050772 TAS
    negative chemotaxis GO:0050919 IBA
    negative chemotaxis GO:0050919 IDA
    negative chemotaxis GO:0050919 IMP
    induction of negative chemotaxis GO:0050929 IDA
    negative regulation of small GTPase mediated signal transduction GO:0051058 IDA
    response to cortisol GO:0051414 IEP
    ventricular septum morphogenesis GO:0060412 ISS
    apoptotic process involved in luteolysis GO:0061364 IEP
    negative regulation of chemokine-mediated signaling pathway GO:0070100 IMP
    cellular response to heparin GO:0071504 IDA
    negative regulation of smooth muscle cell chemotaxis GO:0071672 IDA
    negative regulation of mononuclear cell migration GO:0071676 IDA
    negative regulation of neutrophil chemotaxis GO:0090024 IDA
    negative regulation of monocyte chemotaxis GO:0090027 ISS
    negative regulation of retinal ganglion cell axon guidance GO:0090260 IDA
    negative regulation of cellular response to growth factor stimulus GO:0090288 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IDA
    cell surface GO:0009986 IDA
    membrane GO:0016020 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SLIT2 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLIT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 FBXO2 26232
Affinity Capture-MS Homo sapiens
3 EXOSC7 23016
Affinity Capture-MS Homo sapiens
4 PRICKLE3  
Affinity Capture-MS Homo sapiens
5 PRG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GLI4  
Affinity Capture-MS Homo sapiens
8 KPNA1 3836
Affinity Capture-MS Homo sapiens
9 TP53BP1 7158
Affinity Capture-MS Homo sapiens
10 Robo1  
Affinity Capture-Western Rattus norvegicus
11 ANKRD12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RPS21 6227
Affinity Capture-MS Homo sapiens
13 NOTCH2 4853
Affinity Capture-MS Homo sapiens
14 LUM 4060
Affinity Capture-MS Homo sapiens
15 USP36  
Affinity Capture-MS Homo sapiens
16 GPC1 2817
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 PDGFRA 5156
Affinity Capture-MS Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 BRD2  
Affinity Capture-MS Homo sapiens
20 UBC 7316
Reconstituted Complex Homo sapiens
21 EXOSC9 5393
Affinity Capture-MS Homo sapiens
22 LRRC47 57470
Affinity Capture-MS Homo sapiens
23 C16orf72 29035
Affinity Capture-MS Homo sapiens
24 RNF208  
Affinity Capture-MS Homo sapiens
25 APEX1 328
Affinity Capture-RNA Homo sapiens
26 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
27 MSMB  
Affinity Capture-MS Homo sapiens
28 MKRN2 23609
Affinity Capture-RNA Homo sapiens
29 C1QTNF7  
Affinity Capture-MS Homo sapiens
30 MYC  
Affinity Capture-MS Homo sapiens
31 ROBO4  
Affinity Capture-Western Homo sapiens
32 OGT 8473
Reconstituted Complex Homo sapiens
33 SCGB2A2  
Affinity Capture-MS Homo sapiens
34 ZCCHC10  
Affinity Capture-MS Homo sapiens
35 HUWE1 10075
Affinity Capture-MS Homo sapiens
36 ARRDC3 57561
Affinity Capture-MS Homo sapiens
37 NPM1 4869
Affinity Capture-MS Homo sapiens
38 EXOSC2 23404
Affinity Capture-MS Homo sapiens
39 CSNK1E 1454
Affinity Capture-MS Homo sapiens
40 ST14 6768
Affinity Capture-MS Homo sapiens
41 PTCH1  
Affinity Capture-MS Homo sapiens
42 CANX 821
Affinity Capture-MS Homo sapiens
43 RSRC1  
Affinity Capture-MS Homo sapiens
44 SEC61B 10952
Proximity Label-MS Homo sapiens
45 EXOSC4 54512
Affinity Capture-MS Homo sapiens
46 CCDC8  
Affinity Capture-MS Homo sapiens
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