Experiment description of studies that identified DDX27 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 254 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name MNT-1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 255 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name G1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 256 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 501mel Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 257 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for DDX27

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RPL11 6135 Affinity Capture-MS Homo sapiens 2 CDC14B 8555 Proximity Label-MS Homo sapiens 3 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 4 ZNF668   Affinity Capture-MS Homo sapiens 5 RPSAP58 388524 Affinity Capture-MS Homo sapiens 6 RPSA 3921 Affinity Capture-MS Homo sapiens 7 SENP3 26168 Affinity Capture-MS Homo sapiens 8 KLF8   Proximity Label-MS Homo sapiens 9 NOP58 51602 Co-fractionation Homo sapiens 10 RPS3A 6189 Affinity Capture-MS Homo sapiens 11 KIAA0020 9933 Co-fractionation Homo sapiens 12 SRPK2 6733 Affinity Capture-MS Homo sapiens 13 LHX2   Proximity Label-MS Homo sapiens 14 RPL13A 23521 Affinity Capture-MS Homo sapiens 15 RPL37A 6168 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 NOP56 10528 Co-fractionation Homo sapiens Proximity Label-MS Homo sapiens 17 RPS19 6223 Affinity Capture-MS Homo sapiens 18 RPL10 6134 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 KIF20A 10112 Affinity Capture-MS Homo sapiens 20 RBBP6 5930 Co-fractionation Homo sapiens 21 ZNF71   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 SSB 6741 Co-fractionation Homo sapiens 23 EIF4A1 1973 Cross-Linking-MS (XL-MS) Homo sapiens 24 PNMAL1   Affinity Capture-MS Homo sapiens 25 SRP72 6731 Co-fractionation Homo sapiens 26 Ktn1   Affinity Capture-MS Mus musculus 27 SOX2   Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 DNAJB6 10049 Proximity Label-MS Homo sapiens 29 APEX1 328 Proximity Label-MS Homo sapiens 30 DDX23 9416 Proximity Label-MS Homo sapiens 31 GPATCH4 54865 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 RPL10A 4736 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 ETV3   Proximity Label-MS Homo sapiens 34 PRR11   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 36 RPL28 6158 Affinity Capture-MS Homo sapiens 37 KIF23 9493 Affinity Capture-MS Homo sapiens 38 DDX24 57062 Co-fractionation Homo sapiens 39 ZNF524   Affinity Capture-MS Homo sapiens 40 CCDC137   Affinity Capture-MS Homo sapiens 41 PRC1 9055 Affinity Capture-MS Homo sapiens 42 NPM1 4869 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 ANOS1 3730 Affinity Capture-MS Homo sapiens 44 RPS8 6202 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 FBL 2091 Co-fractionation Homo sapiens Proximity Label-MS Homo sapiens 46 YWHAG 7532 Two-hybrid Homo sapiens 47 KLF15   Proximity Label-MS Homo sapiens 48 OBSL1 23363 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 RPS9 6203 Affinity Capture-MS Homo sapiens 50 RUVBL1 8607 Co-fractionation Homo sapiens 51 ADARB1 104 Affinity Capture-MS Homo sapiens 52 PRKRA 8575 Affinity Capture-MS Homo sapiens 53 EED   Affinity Capture-MS Homo sapiens 54 SRP68 6730 Co-fractionation Homo sapiens 55 NASP 4678 Cross-Linking-MS (XL-MS) Homo sapiens 56 RRP8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 E4F1   Affinity Capture-MS Homo sapiens 58 WDR12 55759 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 59 KLHL20   Affinity Capture-MS Homo sapiens 60 UBE2H 7328 Affinity Capture-MS Homo sapiens 61 MYCN   Affinity Capture-MS Homo sapiens 62 OASL 8638 Affinity Capture-MS Homo sapiens 63 ASF1A 25842 Proximity Label-MS Homo sapiens 64 MAK16   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 65 RPL19 6143 Affinity Capture-MS Homo sapiens 66 DYNLT1 6993 Proximity Label-MS Homo sapiens 67 RBM19 9904 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 68 PES1 23481 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-Western Homo sapiens 69 KLF9 687 Proximity Label-MS Homo sapiens 70 KLF16   Proximity Label-MS Homo sapiens 71 CHMP4C 92421 Affinity Capture-MS Homo sapiens 72 KLF5   Proximity Label-MS Homo sapiens 73 RPL30 6156 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 74 NOP2 4839 Affinity Capture-MS Homo sapiens 75 UTP14A 10813 Co-fractionation Homo sapiens 76 RNF4 6047 Affinity Capture-MS Homo sapiens 77 DDX21 9188 Affinity Capture-MS Homo sapiens 78 LIN28B   Affinity Capture-MS Homo sapiens 79 RPL9 6133 Co-fractionation Homo sapiens 80 RPL4 6124 Affinity Capture-MS Homo sapiens 81 TARBP2   Affinity Capture-MS Homo sapiens 82 RPS10 6204 Affinity Capture-MS Homo sapiens 83 RBM4B   Affinity Capture-MS Homo sapiens 84 YBX2 51087 Affinity Capture-MS Homo sapiens 85 PPP1R16A 84988 Two-hybrid Homo sapiens 86 STAU1 6780 Affinity Capture-MS Homo sapiens 87 APOBEC3C 27350 Affinity Capture-MS Homo sapiens 88 SRSF6 6431 Affinity Capture-MS Homo sapiens 89 PURG   Affinity Capture-MS Homo sapiens 90 ZBTB48   Affinity Capture-MS Homo sapiens 91 RPL5 6125 Co-fractionation Homo sapiens 92 HIST1H2AM 8336 Affinity Capture-MS Homo sapiens 93 RPL18A 6142 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 94 LHX1   Proximity Label-MS Homo sapiens 95 Srp72   Affinity Capture-MS Mus musculus 96 RPA2 6118 Proximity Label-MS Homo sapiens 97 NTRK1 4914 Affinity Capture-MS Homo sapiens 98 RPL31 6160 Co-fractionation Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 99 IFI16 3428 Affinity Capture-MS Homo sapiens 100 HIST1H1E 3008 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 101 REXO4   Affinity Capture-MS Homo sapiens 102 SYNCRIP 10492 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 103 KLF6   Proximity Label-MS Homo sapiens 104 CBX6   Affinity Capture-MS Homo sapiens 105 HIST1H1C 3006 Affinity Capture-MS Homo sapiens 106 LYAR 55646 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 107 RPL36 25873 Affinity Capture-MS Homo sapiens 108 RPS6 6194 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 109 HECTD1 25831 Affinity Capture-MS Homo sapiens 110 RPLP0 6175 Affinity Capture-MS Homo sapiens 111 SRPK3   Affinity Capture-MS Homo sapiens 112 MAGEB2 4113 Affinity Capture-MS Homo sapiens 113 MYBBP1A 10514 Affinity Capture-MS Homo sapiens 114 KLF12   Proximity Label-MS Homo sapiens 115 GLTSCR2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 116 CD3EAP   Proximity Label-MS Homo sapiens 117 WDR46   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 118 CNBP 7555 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 119 ANLN 54443 Affinity Capture-MS Homo sapiens 120 FGFBP1 9982 Affinity Capture-MS Homo sapiens 121 TLX2   Proximity Label-MS Homo sapiens 122 RPL23A 6147 Affinity Capture-MS Homo sapiens 123 FANCD2   Affinity Capture-MS Homo sapiens 124 ABT1 29777 Affinity Capture-MS Homo sapiens 125 RPS24 6229 Proximity Label-MS Homo sapiens 126 RPL7A 6130 Affinity Capture-MS Homo sapiens 127 RPS2 6187 Affinity Capture-MS Homo sapiens 128 EIF6 3692 Co-fractionation Homo sapiens 129 SIRT7   Affinity Capture-MS Homo sapiens 130 SRSF4 6429 Affinity Capture-MS Homo sapiens 131 SRSF1 6426 Affinity Capture-MS Homo sapiens 132 LHX3   Proximity Label-MS Homo sapiens 133 NPM3 10360 Affinity Capture-MS Homo sapiens 134 RPL14 9045 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 135 NR3C1 2908 Proximity Label-MS Homo sapiens 136 DCAF13 25879 Co-fractionation Homo sapiens 137 SRSF5 6430 Affinity Capture-MS Homo sapiens 138 RPL3 6122 Affinity Capture-MS Homo sapiens 139 RPL7L1 285855 Affinity Capture-MS Homo sapiens 140 PDCD11 22984 Affinity Capture-MS Homo sapiens 141 RRP15   Co-fractionation Homo sapiens 142 ZC3H10   Affinity Capture-MS Homo sapiens 143 SOX15   Proximity Label-MS Homo sapiens 144 ZNF512   Affinity Capture-MS Homo sapiens 145 HIST1H2AB 8335 Affinity Capture-MS Homo sapiens 146 HIST1H1B 3009 Affinity Capture-MS Homo sapiens 147 DGKZ   Affinity Capture-MS Homo sapiens 148 DKC1 1736 Co-fractionation Homo sapiens 149 RPA3 6119 Proximity Label-MS Homo sapiens 150 ATP5E 514 Cross-Linking-MS (XL-MS) Homo sapiens 151 ZNF692   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 152 DNAJC9 23234 Proximity Label-MS Homo sapiens 153 NOL12 79159 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 154 RPS14 6208 Affinity Capture-MS Homo sapiens 155 RPL22 6146 Affinity Capture-MS Homo sapiens 156 HIST1H1A 3024 Affinity Capture-MS Homo sapiens 157 CUL7 9820 Affinity Capture-MS Homo sapiens 158 RPL18 6141 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 159 DDRGK1 65992 Affinity Capture-MS Homo sapiens 160 RNF2   Affinity Capture-MS Homo sapiens 161 ZC3HAV1 56829 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 162 CCDC140   Affinity Capture-MS Homo sapiens 163 HIST1H1T 3010 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 164 RPL26 6154 Affinity Capture-MS Homo sapiens 165 ZC3H3   Affinity Capture-MS Homo sapiens 166 FTSJ3 117246 Co-fractionation Homo sapiens 167 RPS16 6217 Affinity Capture-MS Homo sapiens 168 RPL13 6137 Affinity Capture-MS Homo sapiens 169 SURF6   Affinity Capture-MS Homo sapiens 170 ARRB2 409 Affinity Capture-MS Homo sapiens 171 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 172 ZNFX1 57169 Affinity Capture-MS Homo sapiens 173 SIRT6   Affinity Capture-MS Homo sapiens 174 PARP1 142 Proximity Label-MS Homo sapiens 175 HMGN4   Affinity Capture-MS Homo sapiens 176 ZBTB47   Affinity Capture-MS Homo sapiens 177 TP53 7157 Affinity Capture-MS Homo sapiens 178 ZNF346   Affinity Capture-MS Homo sapiens 179 MNDA 4332 Affinity Capture-MS Homo sapiens 180 MCM5 4174 Affinity Capture-MS Homo sapiens 181 RNF151   Affinity Capture-MS Homo sapiens 182 USP36   Affinity Capture-MS Homo sapiens 183 MEN1 4221 Affinity Capture-MS Homo sapiens 184 BOP1 23246 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 185 DGCR8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 186 SMURF1 57154 Affinity Capture-MS Homo sapiens 187 ZNF48   Affinity Capture-MS Homo sapiens 188 UTP23   Affinity Capture-MS Homo sapiens 189 PTBP3 9991 Affinity Capture-MS Homo sapiens 190 H2AFB3   Affinity Capture-MS Homo sapiens 191 MKI67   Affinity Capture-MS Homo sapiens 192 NIFK 84365 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Co-fractionation Homo sapiens 193 RPL35 11224 Affinity Capture-MS Homo sapiens 194 ESR1   Affinity Capture-MS Homo sapiens 195 DHX40   Proximity Label-MS Homo sapiens 196 KRR1 11103 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 197 NEIL1   Affinity Capture-MS Homo sapiens 198 ZNF746   Affinity Capture-MS Homo sapiens 199 MED12   Affinity Capture-MS Homo sapiens 200 API5 8539 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 201 IL17B 27190 Affinity Capture-MS Homo sapiens 202 TECTB   Affinity Capture-MS Homo sapiens 203 PARK2   Affinity Capture-MS Homo sapiens 204 ARRB1 408 Affinity Capture-MS Homo sapiens 205 HSF1   Affinity Capture-MS Homo sapiens 206 RPL23 9349 Co-fractionation Homo sapiens 207 RPL23AP32 56969 Affinity Capture-MS Homo sapiens 208 Rrbp1   Affinity Capture-MS Mus musculus 209 RPL8 6132 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 210 GRSF1 2926 Proximity Label-MS Homo sapiens 211 PPAN 56342 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 212 KRAS 3845 Synthetic Lethality Homo sapiens Negative Genetic Homo sapiens 213 HNRNPU 3192 Affinity Capture-MS Homo sapiens 214 RBM34   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 215 ZNF467   Affinity Capture-MS Homo sapiens 216 RPL17 6139 Affinity Capture-MS Homo sapiens 217 EP300 2033 Affinity Capture-MS Homo sapiens 218 NHLH1   Proximity Label-MS Homo sapiens View the network

Pathways in which DDX27 is involved

No pathways found